A multidisciplinary study to assess the ecology of the Cape sea urchin, Parechinus angulosus, and its emerging use as a bioindicator to monitor coastal resiliency
- Authors: Redelinghuys, Suzanne
- Date: 2024-04-05
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/435691 , vital:73179 , DOI 10.21504/10962/435692
- Description: The resilience of marine species in the face of unpredictable climate change stands as a paramount concern for the maintenance of ecological stability. Under such fluctuating conditions, it is critical to understand how organisms mitigate these effects in physiological, genetic, and morphological terms. To that end, this thesis focused on the Cape sea urchin, Parechinus angulosus, employing a multidisciplinary approach encompassing morphology, genomics, and gut microbial diversity to assess its potential as a bioindicator species and elucidate its adaptive strategies in response to varying environmental conditions along the South African coastline. This was achieved through studying their anatomy in order to link observed variation to prevailing local environmental conditions, aided by the species’ wide distribution range which allows insight into adaptations across broad geographic regions and ecological settings. The first empirical chapter, Chapter 3, focusing on eight key morphometric traits of test, Aristotle’s lantern and spines, revealed distinct variation in the Cape sea urchin's morphology between the east and west coasts of South Africa, suggesting the presence of some level of local adaptation to the prevailing environmental factors found on the east and west coasts of South Africa. This points to potential bioindicator capabilities of the species, reflecting adaptive divergence amidst contrasting environmental conditions. Further analysis is however necessary in order to isolate specific physiological trends that may be associated with these morphometric differences, thereby enhancing and tightening their ecological implications. The second experimental chapter, Chapter 4, delved into the genetic structure of the Cape urchin by investigating genome-wide diversity, the presence of cryptic population structure, and spatial patterns of genomic diversity. Moderate genomic differentiation was detected among populations along the eastern and western coasts of South Africa by outlier loci that may undergo natural selection, which could indicate local adaptation to environmental conditions. This pattern hints at adaptive differentiation and cryptic genetic structures within the Cape sea urchin populations and emphasises the species' potential adaptive responses to localised (in this case regional) environmental pressures. Assigning functional significance to these genetic variations will require a comprehensive annotated reference genome, a limitation acknowledged in the current study. Chapter 5 explored the gut microbial diversity and revealed significant compositional variations between the east and west coast populations of South Africa, confirming regional and inter-regional variation. This chapter also highlighted the essential biochemical pathways critical to the survival of the host which is crucial for assessing the health of the urchin host. Together, the functional content of the gut bacteria and microbial diversity showcases its potential as a bioindicator for coastal ecosystem health. Logistical challenges and confounding factors like host physiology will need to be fully considered for its effective application. Overall, the findings of this doctoral research suggest that the Cape sea urchin displays promising characteristics as a bioindicator species due to its morphological, genetic, and gut microbial variations in response to environmental differences, providing a diverse array of means in which urchins could be used as bioindicators, from their uses to assess water quality and detect pollution, to ecosystem health monitoring and biodiversity studies in which sea urchin abundance, distribution, and presence are monitored. Further research, integrating these multidisciplinary approaches is recommended to validate and refine its bioindicator potential. Additionally, the development of a comprehensive annotated reference genome is imperative to harness the species' genetic information effectively. This study underscores the significance of integrating multiple disciplines in understanding how species respond to environmental change and their potentials contributions to monitor ecological resilience. The original multidisciplinary approach, combined with high computational outputs presents a promising framework for a comprehensive ecological monitoring in marine ecosystems. , Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-05
- Authors: Redelinghuys, Suzanne
- Date: 2024-04-05
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/435691 , vital:73179 , DOI 10.21504/10962/435692
- Description: The resilience of marine species in the face of unpredictable climate change stands as a paramount concern for the maintenance of ecological stability. Under such fluctuating conditions, it is critical to understand how organisms mitigate these effects in physiological, genetic, and morphological terms. To that end, this thesis focused on the Cape sea urchin, Parechinus angulosus, employing a multidisciplinary approach encompassing morphology, genomics, and gut microbial diversity to assess its potential as a bioindicator species and elucidate its adaptive strategies in response to varying environmental conditions along the South African coastline. This was achieved through studying their anatomy in order to link observed variation to prevailing local environmental conditions, aided by the species’ wide distribution range which allows insight into adaptations across broad geographic regions and ecological settings. The first empirical chapter, Chapter 3, focusing on eight key morphometric traits of test, Aristotle’s lantern and spines, revealed distinct variation in the Cape sea urchin's morphology between the east and west coasts of South Africa, suggesting the presence of some level of local adaptation to the prevailing environmental factors found on the east and west coasts of South Africa. This points to potential bioindicator capabilities of the species, reflecting adaptive divergence amidst contrasting environmental conditions. Further analysis is however necessary in order to isolate specific physiological trends that may be associated with these morphometric differences, thereby enhancing and tightening their ecological implications. The second experimental chapter, Chapter 4, delved into the genetic structure of the Cape urchin by investigating genome-wide diversity, the presence of cryptic population structure, and spatial patterns of genomic diversity. Moderate genomic differentiation was detected among populations along the eastern and western coasts of South Africa by outlier loci that may undergo natural selection, which could indicate local adaptation to environmental conditions. This pattern hints at adaptive differentiation and cryptic genetic structures within the Cape sea urchin populations and emphasises the species' potential adaptive responses to localised (in this case regional) environmental pressures. Assigning functional significance to these genetic variations will require a comprehensive annotated reference genome, a limitation acknowledged in the current study. Chapter 5 explored the gut microbial diversity and revealed significant compositional variations between the east and west coast populations of South Africa, confirming regional and inter-regional variation. This chapter also highlighted the essential biochemical pathways critical to the survival of the host which is crucial for assessing the health of the urchin host. Together, the functional content of the gut bacteria and microbial diversity showcases its potential as a bioindicator for coastal ecosystem health. Logistical challenges and confounding factors like host physiology will need to be fully considered for its effective application. Overall, the findings of this doctoral research suggest that the Cape sea urchin displays promising characteristics as a bioindicator species due to its morphological, genetic, and gut microbial variations in response to environmental differences, providing a diverse array of means in which urchins could be used as bioindicators, from their uses to assess water quality and detect pollution, to ecosystem health monitoring and biodiversity studies in which sea urchin abundance, distribution, and presence are monitored. Further research, integrating these multidisciplinary approaches is recommended to validate and refine its bioindicator potential. Additionally, the development of a comprehensive annotated reference genome is imperative to harness the species' genetic information effectively. This study underscores the significance of integrating multiple disciplines in understanding how species respond to environmental change and their potentials contributions to monitor ecological resilience. The original multidisciplinary approach, combined with high computational outputs presents a promising framework for a comprehensive ecological monitoring in marine ecosystems. , Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-05
Temporal patterns and seasonal variation in microplastic loads in the water column and in the tissues of consumers along the southern and south-eastern coasts of South Africa
- Authors: Redelinghuys, Suzanne
- Date: 2020
- Subjects: Microplastics -- South Africa -- Eastern Cape , Plastic marine debris -- South Africa -- Eastern Cape , Marine pollution -- South Africa -- Eastern Cape , Ocean circulation -- South Africa -- Eastern Cape , Marine invertebrates -- Effect of pollution on -- South Africa -- Eastern Cape , Marine animals -- Effect of pollution on -- South Africa -- Eastern Cape , Mexilhao mussel -- Effect of pollution on -- South Africa -- Eastern Cape , Mytilus galloprovincialis -- Effect of pollution on -- South Africa -- Eastern Cape , Barnacles -- Effect of pollution on -- South Africa -- Eastern Cape , Tetraclita -- Effect of pollution on -- South Africa -- Eastern Cape , Octomeris angulosa -- Effect of pollution on -- South Africa -- Eastern Cape
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/115623 , vital:34201
- Description: Plastic pollution in the marine environment has become an environmental concern and a subject of ecological research. The field of microplastic pollution in particular has expanded dramatically in the last few years. Though much data exists on the spatial variability of microplastics in the marine environment globally, little is known about temporal variability, especially on short-term time scales in the southern hemisphere. Similarly, virtually nothing is known about the temporal patterns in microplastic ingestion by marine invertebrates, despite the fact that numerous studies have demonstrated that vertebrates and invertebrates routinely ingest microplastics with varied physiological effects. This study aimed to, 1) provide base-line data for microplastic loads in the nearshore environment along the Eastern Cape Province of South Africa over four short-term time scales: daily, weekly, monthly, and yearly; and, 2) assess whether there are any seasonal patterns in microplastics ingested by selected filter-feeding consumers at two sites along the southern and south-eastern Cape coastlines of South Africa. Results for part one of this study demonstrate no temporal patterns over the different time scales considered (ANOVA, p > 0.05 in all cases). Microplastic counts ranged on average from 55 ± 289 to 930 ± 462 microplastic particles.m-3. With the exception of two instances, microfibres constituted > 50 % (range: 47 to 97 %) of the total microplastic counts. Part two of this study assessed the size range of, and seasonal and spatial patterns in ingested microplastic. No significant differences were found in the number of microplastics ingested within seasons between the mussels Perna perna (Linnaeus, 1758) and Mytilus galloprovincialis (Lamarck, 1819), and the barnacles, Octomeris angulosa (Sowerby, 1825) and Tetraclita serrata (Darwin 1954) (Student’s t-test; d.f = 18; p > 0.05 in all cases), or between the two sites sampled, Kenton-on-Sea, Eastern Cape, and Wilderness, Western Cape (ANOVA; d.f. = 18; p > 0.05 in all cases). The nitric acid digestion technique was used to determine the presence of ingested microplastics. Microplastic loads ranged from 2 ± 1 to 33 ± 19 microplastics.g-1 wwt across all consumers, and the size of ingested microplastics ranged from 1 to 16 μm. Though highly variable, the absence of statistically significant differences in ingestion rates points to a ubiquity in the availability of microplastics within the water column over time and space.
- Full Text:
- Date Issued: 2020
- Authors: Redelinghuys, Suzanne
- Date: 2020
- Subjects: Microplastics -- South Africa -- Eastern Cape , Plastic marine debris -- South Africa -- Eastern Cape , Marine pollution -- South Africa -- Eastern Cape , Ocean circulation -- South Africa -- Eastern Cape , Marine invertebrates -- Effect of pollution on -- South Africa -- Eastern Cape , Marine animals -- Effect of pollution on -- South Africa -- Eastern Cape , Mexilhao mussel -- Effect of pollution on -- South Africa -- Eastern Cape , Mytilus galloprovincialis -- Effect of pollution on -- South Africa -- Eastern Cape , Barnacles -- Effect of pollution on -- South Africa -- Eastern Cape , Tetraclita -- Effect of pollution on -- South Africa -- Eastern Cape , Octomeris angulosa -- Effect of pollution on -- South Africa -- Eastern Cape
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/115623 , vital:34201
- Description: Plastic pollution in the marine environment has become an environmental concern and a subject of ecological research. The field of microplastic pollution in particular has expanded dramatically in the last few years. Though much data exists on the spatial variability of microplastics in the marine environment globally, little is known about temporal variability, especially on short-term time scales in the southern hemisphere. Similarly, virtually nothing is known about the temporal patterns in microplastic ingestion by marine invertebrates, despite the fact that numerous studies have demonstrated that vertebrates and invertebrates routinely ingest microplastics with varied physiological effects. This study aimed to, 1) provide base-line data for microplastic loads in the nearshore environment along the Eastern Cape Province of South Africa over four short-term time scales: daily, weekly, monthly, and yearly; and, 2) assess whether there are any seasonal patterns in microplastics ingested by selected filter-feeding consumers at two sites along the southern and south-eastern Cape coastlines of South Africa. Results for part one of this study demonstrate no temporal patterns over the different time scales considered (ANOVA, p > 0.05 in all cases). Microplastic counts ranged on average from 55 ± 289 to 930 ± 462 microplastic particles.m-3. With the exception of two instances, microfibres constituted > 50 % (range: 47 to 97 %) of the total microplastic counts. Part two of this study assessed the size range of, and seasonal and spatial patterns in ingested microplastic. No significant differences were found in the number of microplastics ingested within seasons between the mussels Perna perna (Linnaeus, 1758) and Mytilus galloprovincialis (Lamarck, 1819), and the barnacles, Octomeris angulosa (Sowerby, 1825) and Tetraclita serrata (Darwin 1954) (Student’s t-test; d.f = 18; p > 0.05 in all cases), or between the two sites sampled, Kenton-on-Sea, Eastern Cape, and Wilderness, Western Cape (ANOVA; d.f. = 18; p > 0.05 in all cases). The nitric acid digestion technique was used to determine the presence of ingested microplastics. Microplastic loads ranged from 2 ± 1 to 33 ± 19 microplastics.g-1 wwt across all consumers, and the size of ingested microplastics ranged from 1 to 16 μm. Though highly variable, the absence of statistically significant differences in ingestion rates points to a ubiquity in the availability of microplastics within the water column over time and space.
- Full Text:
- Date Issued: 2020
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