Anticholinesterase and Antioxidant Activities of Spilanthes filicaulis Whole Plant Extracts for the Management of Alzheimer’s Disease
- Elufioye, Taiwo O., Unachukwu, Cynthia C., Oyedeji, Adebola O.
- Authors: Elufioye, Taiwo O. , Unachukwu, Cynthia C. , Oyedeji, Adebola O.
- Date: 2019
- Subjects: Alzheimer’s disease , Cholinesterase inhibitors , Antioxidants , Spilanthes , Flavonoids , Phenolic
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/6534 , vital:46450 , xlink:href="https://10.2174/1573408015666190730113405"
- Description: Background: Spilanthes filicaulis is a tropical herb implicated as a memory enhancer in ethnomedicine. Objective: The study investigated acetyl/butyryl cholinesterase inhibitory and antioxidant activities of different extracts of S. filicaulis whole plant and correlated them to its phytochemical constituents. Methods: The powdered whole plant was successively extracted with n-hexane, ethyl acetate and methanol. Acetyl cholinesterase (AChE) and Butyryl cholinesterase (BuChE) inhibitory activity were evaluated by Ellman colorimetry assay. Antioxidant activity was tested using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, ferric reducing power and nitric oxide scavenging assays. Total phenolic, flavonoid and tannin were estimated using standard methods. Correlation was determined using Quest Graph™ Regression Calculator. Results: Various extracts exhibited concentration-dependent AChE and BuChE inhibitory activity with ethyl acetate extract being the highest with IC50 of 0.77 μg/mL and 0.92 μg/mL for AChE and BuChE respectively. The ethyl acetate extract also showed the highest reducing power when compared with the other extracts. The methanol extract had slightly higher phenolic and flavonoid content and showed the highest DPPH radical scavenging effect. DPPH scavenging, AChE and BuChE inhibition had high correlation with the total flavonoid content with R2 values of 1.00, 0.800 and 0.992 respectively while nitric oxide scavenging had high correlation with phenolics and tannins with R2 = 0.942 and 0.806 respectively. Conclusion: These results show that the extracts of the whole plant of S. filicaulis possess significant AChE/BuChE inhibitory and antioxidant properties, mostly due to its flavonoid content, suggesting the possible use of the plant in neurodegenerative diseases such as AD.
- Full Text:
- Authors: Elufioye, Taiwo O. , Unachukwu, Cynthia C. , Oyedeji, Adebola O.
- Date: 2019
- Subjects: Alzheimer’s disease , Cholinesterase inhibitors , Antioxidants , Spilanthes , Flavonoids , Phenolic
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/6534 , vital:46450 , xlink:href="https://10.2174/1573408015666190730113405"
- Description: Background: Spilanthes filicaulis is a tropical herb implicated as a memory enhancer in ethnomedicine. Objective: The study investigated acetyl/butyryl cholinesterase inhibitory and antioxidant activities of different extracts of S. filicaulis whole plant and correlated them to its phytochemical constituents. Methods: The powdered whole plant was successively extracted with n-hexane, ethyl acetate and methanol. Acetyl cholinesterase (AChE) and Butyryl cholinesterase (BuChE) inhibitory activity were evaluated by Ellman colorimetry assay. Antioxidant activity was tested using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, ferric reducing power and nitric oxide scavenging assays. Total phenolic, flavonoid and tannin were estimated using standard methods. Correlation was determined using Quest Graph™ Regression Calculator. Results: Various extracts exhibited concentration-dependent AChE and BuChE inhibitory activity with ethyl acetate extract being the highest with IC50 of 0.77 μg/mL and 0.92 μg/mL for AChE and BuChE respectively. The ethyl acetate extract also showed the highest reducing power when compared with the other extracts. The methanol extract had slightly higher phenolic and flavonoid content and showed the highest DPPH radical scavenging effect. DPPH scavenging, AChE and BuChE inhibition had high correlation with the total flavonoid content with R2 values of 1.00, 0.800 and 0.992 respectively while nitric oxide scavenging had high correlation with phenolics and tannins with R2 = 0.942 and 0.806 respectively. Conclusion: These results show that the extracts of the whole plant of S. filicaulis possess significant AChE/BuChE inhibitory and antioxidant properties, mostly due to its flavonoid content, suggesting the possible use of the plant in neurodegenerative diseases such as AD.
- Full Text:
Antihypertensive effects of the hydroethanol extract of Senecio serratuloides DC in rats
- Tata, Charlotte Mungho, Sewani-Rusike, Constance Rufaro, Oyedeji, Opeoluwa Oyehan, Gwebu, Ephraim Tobela, Mahlakata, Fikile, Nkeh-Chungag, Benedicta Ngwenchi
- Authors: Tata, Charlotte Mungho , Sewani-Rusike, Constance Rufaro , Oyedeji, Opeoluwa Oyehan , Gwebu, Ephraim Tobela , Mahlakata, Fikile , Nkeh-Chungag, Benedicta Ngwenchi
- Date: 2019
- Subjects: South Africa Hypertension Computer File
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/5847 , vital:44656 , https://doi.org/10.1186/s12906-019-2463-2
- Description: Senecio serratuloides DC is used in folk medicine for treating hypertension, skin disorders, internal and external sores, rashes, burns and wounds. This study aimed at investigating the antihypertensive effects of the hydroethanol extract of S. serratuloides (HESS) in N-Nitro-L-arginine methyl ester (L-NAME) induced hypertension in rats. Methods: Acute toxicity of HESS was first determined to provide guidance on doses to be used in this study. Lorke’s method was used to determine safety of the extract in mice. Female Wistar rats were treated orally once daily with L-NAME (40 mg/kg) for 4 weeks and then concomitantly with L-NAME (20 mg/kg) and plant extract (150 and 300 mg/kg), captopril (20 mg/kg) or saline as per assigned group for 2 weeks followed by a 2-week period of assigned treatments only. Blood pressure was monitored weekly. Lipid profile, nitric oxide, renin and angiotensin II concentrations were determined in serum while mineralocorticoid receptor concentration was quantified in the kidney homogenate. Nitric oxide (NO) concentration was determined in serum and cardiac histology performed. Results HESS was found to be non-toxic, having a LD50 greater than 5000 mg/kg. Blood pressure increased progressively in all animals from the second week of L-NAME treatment. HESS treatment significantly and dose-dependently lowered systolic blood pressure (p less 0.001), diastolic blood pressure (p less 0.01), low density lipoprotein cholesterol (p less 0.01) and triglycerides (p less 0.01). It significantly prevented L-NAME induced decrease in serum angiotensin II (p less 0.01), high density lipoprotein cholesterol (p less 0.001) and serum nitric oxide concentrations (p less 0.001). HESS also significantly (p less 0.01) prevented collagen deposition in cardiac tissue. Conclusion The hydro-ethanol extract of Senecio serratuloides showed antihypertensive, antihyperlipidemic and cardioprotective effects in rats thus confirming its usefulness in traditional antihypertensive therapy and potential for antihypertensive drug development.
- Full Text:
- Authors: Tata, Charlotte Mungho , Sewani-Rusike, Constance Rufaro , Oyedeji, Opeoluwa Oyehan , Gwebu, Ephraim Tobela , Mahlakata, Fikile , Nkeh-Chungag, Benedicta Ngwenchi
- Date: 2019
- Subjects: South Africa Hypertension Computer File
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/5847 , vital:44656 , https://doi.org/10.1186/s12906-019-2463-2
- Description: Senecio serratuloides DC is used in folk medicine for treating hypertension, skin disorders, internal and external sores, rashes, burns and wounds. This study aimed at investigating the antihypertensive effects of the hydroethanol extract of S. serratuloides (HESS) in N-Nitro-L-arginine methyl ester (L-NAME) induced hypertension in rats. Methods: Acute toxicity of HESS was first determined to provide guidance on doses to be used in this study. Lorke’s method was used to determine safety of the extract in mice. Female Wistar rats were treated orally once daily with L-NAME (40 mg/kg) for 4 weeks and then concomitantly with L-NAME (20 mg/kg) and plant extract (150 and 300 mg/kg), captopril (20 mg/kg) or saline as per assigned group for 2 weeks followed by a 2-week period of assigned treatments only. Blood pressure was monitored weekly. Lipid profile, nitric oxide, renin and angiotensin II concentrations were determined in serum while mineralocorticoid receptor concentration was quantified in the kidney homogenate. Nitric oxide (NO) concentration was determined in serum and cardiac histology performed. Results HESS was found to be non-toxic, having a LD50 greater than 5000 mg/kg. Blood pressure increased progressively in all animals from the second week of L-NAME treatment. HESS treatment significantly and dose-dependently lowered systolic blood pressure (p less 0.001), diastolic blood pressure (p less 0.01), low density lipoprotein cholesterol (p less 0.01) and triglycerides (p less 0.01). It significantly prevented L-NAME induced decrease in serum angiotensin II (p less 0.01), high density lipoprotein cholesterol (p less 0.001) and serum nitric oxide concentrations (p less 0.001). HESS also significantly (p less 0.01) prevented collagen deposition in cardiac tissue. Conclusion The hydro-ethanol extract of Senecio serratuloides showed antihypertensive, antihyperlipidemic and cardioprotective effects in rats thus confirming its usefulness in traditional antihypertensive therapy and potential for antihypertensive drug development.
- Full Text:
Antihypertensive effects of the hydroethanol extract of Senecio serratuloides DC in rats
- Tata, Charlotte Mungho, Sewani-Rusike, Constance Rufaro, Oyedeji, Opeoluwa Oyehan, Gwebu, Ephraim Tobela, Mahlakata, Fikile, Nkeh-Chungag, Benedicta Ngwenchi
- Authors: Tata, Charlotte Mungho , Sewani-Rusike, Constance Rufaro , Oyedeji, Opeoluwa Oyehan , Gwebu, Ephraim Tobela , Mahlakata, Fikile , Nkeh-Chungag, Benedicta Ngwenchi
- Date: 2019
- Subjects: South Africa Hypertension Computer File
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/5842 , vital:44654 , https://doi.org/10.1186/s12906-019-2463-2
- Description: Senecio serratuloides DC is used in folk medicine for treating hypertension, skin disorders, internal and external sores, rashes, burns and wounds. This study aimed at investigating the antihypertensive effects of the hydroethanol extract of S. serratuloides (HESS) in N-Nitro-L-arginine methyl ester (L-NAME) induced hypertension in rats. Methods: Acute toxicity of HESS was first determined to provide guidance on doses to be used in this study. Lorke’s method was used to determine safety of the extract in mice. Female Wistar rats were treated orally once daily with L-NAME (40 mg/kg) for 4 weeks and then concomitantly with L-NAME (20 mg/kg) and plant extract (150 and 300 mg/kg), captopril (20 mg/kg) or saline as per assigned group for 2 weeks followed by a 2-week period of assigned treatments only. Blood pressure was monitored weekly. Lipid profile, nitric oxide, renin and angiotensin II concentrations were determined in serum while mineralocorticoid receptor concentration was quantified in the kidney homogenate. Nitric oxide (NO) concentration was determined in serum and cardiac histology performed. Results HESS was found to be non-toxic, having a LD50 greater than 5000 mg/kg. Blood pressure increased progressively in all animals from the second week of L-NAME treatment. HESS treatment significantly and dose-dependently lowered systolic blood pressure (p less 0.001), diastolic blood pressure (p less 0.01), low density lipoprotein cholesterol (p less 0.01) and triglycerides (p less 0.01). It significantly prevented L-NAME induced decrease in serum angiotensin II (p less 0.01), high density lipoprotein cholesterol (p less 0.001) and serum nitric oxide concentrations (p less 0.001). HESS also significantly (p less 0.01) prevented collagen deposition in cardiac tissue. Conclusion The hydro-ethanol extract of Senecio serratuloides showed antihypertensive, antihyperlipidemic and cardioprotective effects in rats thus confirming its usefulness in traditional antihypertensive therapy and potential for antihypertensive drug development.
- Full Text:
- Authors: Tata, Charlotte Mungho , Sewani-Rusike, Constance Rufaro , Oyedeji, Opeoluwa Oyehan , Gwebu, Ephraim Tobela , Mahlakata, Fikile , Nkeh-Chungag, Benedicta Ngwenchi
- Date: 2019
- Subjects: South Africa Hypertension Computer File
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/5842 , vital:44654 , https://doi.org/10.1186/s12906-019-2463-2
- Description: Senecio serratuloides DC is used in folk medicine for treating hypertension, skin disorders, internal and external sores, rashes, burns and wounds. This study aimed at investigating the antihypertensive effects of the hydroethanol extract of S. serratuloides (HESS) in N-Nitro-L-arginine methyl ester (L-NAME) induced hypertension in rats. Methods: Acute toxicity of HESS was first determined to provide guidance on doses to be used in this study. Lorke’s method was used to determine safety of the extract in mice. Female Wistar rats were treated orally once daily with L-NAME (40 mg/kg) for 4 weeks and then concomitantly with L-NAME (20 mg/kg) and plant extract (150 and 300 mg/kg), captopril (20 mg/kg) or saline as per assigned group for 2 weeks followed by a 2-week period of assigned treatments only. Blood pressure was monitored weekly. Lipid profile, nitric oxide, renin and angiotensin II concentrations were determined in serum while mineralocorticoid receptor concentration was quantified in the kidney homogenate. Nitric oxide (NO) concentration was determined in serum and cardiac histology performed. Results HESS was found to be non-toxic, having a LD50 greater than 5000 mg/kg. Blood pressure increased progressively in all animals from the second week of L-NAME treatment. HESS treatment significantly and dose-dependently lowered systolic blood pressure (p less 0.001), diastolic blood pressure (p less 0.01), low density lipoprotein cholesterol (p less 0.01) and triglycerides (p less 0.01). It significantly prevented L-NAME induced decrease in serum angiotensin II (p less 0.01), high density lipoprotein cholesterol (p less 0.001) and serum nitric oxide concentrations (p less 0.001). HESS also significantly (p less 0.01) prevented collagen deposition in cardiac tissue. Conclusion The hydro-ethanol extract of Senecio serratuloides showed antihypertensive, antihyperlipidemic and cardioprotective effects in rats thus confirming its usefulness in traditional antihypertensive therapy and potential for antihypertensive drug development.
- Full Text:
Biogenic synthesis of Silver nanoparticles (AgNPs) and its effects on Zebrafish (Danio rerio)
- Authors: Siguba, Busiswa
- Date: 2019
- Subjects: Biogenic synthesis -- Zebrafish , Nanoparticles – Silver , Zebrafish
- Language: English
- Type: Thesis , Masters , M Sc
- Identifier: http://hdl.handle.net/11260/1339 , vital:32260
- Description: The increase in use and production of engineered nanoparticles in recent years points towards their inevitable release into freshwater ecosystems. Subsequently, exposure to organisms in freshwater ecosystems is to be expected. The aim of this dissertation was to synthesize silver nanoparticles (AgNPs) using silver nitrate, gelatine and maltose as precursor, capping and reducing agent respectively. Cytotoxic effects of the as-synthesized AgNPs was evaluated at concentrations of 0.031 μg/L, 0.250 μg/L and 5.000 μg/L on selected target organs in zebrafish (Danio rerio ) after 96 hr of exposure, under a 24 hr static renewal system. Formation of AgNPs was investigated by UV-Vis spectrophotometry, which displayed absorption maxima peak characteristic of plasmon resonance band for silver at 419 nm. From transmission electron microscopy, AgNPs was found to be small, well dispersed and spherical in shape with a narrow size distribution, and an average particle size of3.76 ± 1.00 nm. Histopathological alterations following exposure to AgNPs was noticed in the gills, epidermis, muscle tissue and kidney. Secondary lamellae hypertrophy, oedema and epithelial lifting was observed in all experimental groups. Curling and clubbed tips of secondary lamellae were observed at the lowest concentration (0.031 μg/L). Hyperplasia and lamellae fusion was most common at the median concentration (0.250 μg/L) of AgNP exposure. Hypertrophy of endothelial cells along with blood vessel congestion was also observed. Reduced secondary lamellar length, lamellar erosion and thinning were observed at the highest concentration (5.000 μg/L).AgNP exposure also altered the shape of alarm cells. In addition, hyperplasia and hypertrophy of goblet cells were observed at the highest concentration (5.000 μg/L) at 96 hr. At 5.000 μg/L of AgNP, PAS reaction was intense (deep purple), while the number and size of these cells decreased. Staining with AB pH 2.5 demonstrated an intense deep purple staining at the lowest and at the median concentration (24 hr). Moderate staining was seen at the higher concentrations. Muscle bundles with severe oedema, their thickening and separation were more pronounced in all treated groups. Histopathological tubular deformation and mononuclear cell infiltration were observed in kidney tissues. In addition; an increase in melano‐macrophage aggregation intensity was identified on the 48 hr in the treated groups. An increase in tubular epithelial cell regeneration/ proliferation, resulting in production of new epithelial cells which was indicative of recovering of kidney tissues from AgNP induced injury at 96 hr of exposure. Results presented here shows that fish respond to the presence of AgNPs.
- Full Text:
- Authors: Siguba, Busiswa
- Date: 2019
- Subjects: Biogenic synthesis -- Zebrafish , Nanoparticles – Silver , Zebrafish
- Language: English
- Type: Thesis , Masters , M Sc
- Identifier: http://hdl.handle.net/11260/1339 , vital:32260
- Description: The increase in use and production of engineered nanoparticles in recent years points towards their inevitable release into freshwater ecosystems. Subsequently, exposure to organisms in freshwater ecosystems is to be expected. The aim of this dissertation was to synthesize silver nanoparticles (AgNPs) using silver nitrate, gelatine and maltose as precursor, capping and reducing agent respectively. Cytotoxic effects of the as-synthesized AgNPs was evaluated at concentrations of 0.031 μg/L, 0.250 μg/L and 5.000 μg/L on selected target organs in zebrafish (Danio rerio ) after 96 hr of exposure, under a 24 hr static renewal system. Formation of AgNPs was investigated by UV-Vis spectrophotometry, which displayed absorption maxima peak characteristic of plasmon resonance band for silver at 419 nm. From transmission electron microscopy, AgNPs was found to be small, well dispersed and spherical in shape with a narrow size distribution, and an average particle size of3.76 ± 1.00 nm. Histopathological alterations following exposure to AgNPs was noticed in the gills, epidermis, muscle tissue and kidney. Secondary lamellae hypertrophy, oedema and epithelial lifting was observed in all experimental groups. Curling and clubbed tips of secondary lamellae were observed at the lowest concentration (0.031 μg/L). Hyperplasia and lamellae fusion was most common at the median concentration (0.250 μg/L) of AgNP exposure. Hypertrophy of endothelial cells along with blood vessel congestion was also observed. Reduced secondary lamellar length, lamellar erosion and thinning were observed at the highest concentration (5.000 μg/L).AgNP exposure also altered the shape of alarm cells. In addition, hyperplasia and hypertrophy of goblet cells were observed at the highest concentration (5.000 μg/L) at 96 hr. At 5.000 μg/L of AgNP, PAS reaction was intense (deep purple), while the number and size of these cells decreased. Staining with AB pH 2.5 demonstrated an intense deep purple staining at the lowest and at the median concentration (24 hr). Moderate staining was seen at the higher concentrations. Muscle bundles with severe oedema, their thickening and separation were more pronounced in all treated groups. Histopathological tubular deformation and mononuclear cell infiltration were observed in kidney tissues. In addition; an increase in melano‐macrophage aggregation intensity was identified on the 48 hr in the treated groups. An increase in tubular epithelial cell regeneration/ proliferation, resulting in production of new epithelial cells which was indicative of recovering of kidney tissues from AgNP induced injury at 96 hr of exposure. Results presented here shows that fish respond to the presence of AgNPs.
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Comparative study of phytochemical composition and insecticidal properties of Lemon species from Eastern Cape and KwaZulu-Natal
- Authors: Nodola, Phumelele
- Date: 2019
- Subjects: Phytochemical -- Insecticidal properties Lemon - - Species – Eastern Cape - - South Africa Citrus limon
- Language: English
- Type: Thesis , Masters , M Sc
- Identifier: http://hdl.handle.net/11260/1348 , vital:32261
- Description: Citrus limon commonly known as lemon is the third most cultivated citrus genus in South Africa mainly cultivated for its juice. This study aims at analysing the chemical composition of Citrus limon waste (leaf and peel) and evaluation of their insecticidal properties. Leaves and peels of Eureka lemons were collected from three South African citrus farms (Addo, Riverside and Mystic), while Lisbon lemons were only collected from two citrus farms (Addo and Riverside) in 2017 and 2018. A total of 36 essential oils were extracted using the Clevenger like apparatus. Analyses of these oils were done using GCMS. An infusion extract of C. limon was prepared according to the standard method and subjected for phytochemical screening to detect and quantify secondary metabolites present. D-Limonene was the major compound identified in all the 36 oils from Eureka and Lisbon peels and leaves (27.9-74.8%). Other dominant compounds identified in Eureka leaf and peel oils were -pinene (5.8-13.2%), -terpinene (4.8-6.7%), geranial (4.0-15.4%), z-citral (6.7-11.7%), sabinene (4.5-6.3%), nerol (4.0-5.4%) and neryl acetate (6.3-9.5%). Some compounds were however present only in some oil such caryophyllene (6.5%) in Mystic dry leaf oil, geraniol (8.0%) in Addo dry leaf oil, as significant compounds. Lisbon leaf and peel essential oils -pinene (4.4- 11.8%), p-cymene (4.2-6.3%), -terpinene (4.4-6.9%), geranial (8.6-17.3%), z-citral (7.1-13.6%), neryl acetate (4.7-9.1%) (5.4-5.9%) were the other prominent peaks identified in the oils. These C. limon essential oils were further tested for insecticidal activities against maize weevils by conducting repellent assay, fumigant assay and contact toxicity; also plant viii powder was used against maize weevils. In general, for all the bioassay, mortality of the insect increased as the contact days with the oil increased. Contact toxicity assays of C. limon peel essential oils were most effective against S. zeamais in which all weevils were dead by day 3 (100% mortality) at 300 μL, while at other concentrations (50, 100, 200 μL) over 50% mortality was observed. The fumigant potential was high with the peel oil with over 50% mortality at 32 and 40 μL in day 4. The oil had moderate repellent effect on the maize weevil. Riverside Eureka dried powder peel was the most effect in all. These results unveil the medicinal potential of South African citrus waste in post-harvest crop protection and good sources of some chemical compounds which are of medicinal value. Furthermore, this study also reveals the economic potential of these wastes in job creation.
- Full Text:
- Authors: Nodola, Phumelele
- Date: 2019
- Subjects: Phytochemical -- Insecticidal properties Lemon - - Species – Eastern Cape - - South Africa Citrus limon
- Language: English
- Type: Thesis , Masters , M Sc
- Identifier: http://hdl.handle.net/11260/1348 , vital:32261
- Description: Citrus limon commonly known as lemon is the third most cultivated citrus genus in South Africa mainly cultivated for its juice. This study aims at analysing the chemical composition of Citrus limon waste (leaf and peel) and evaluation of their insecticidal properties. Leaves and peels of Eureka lemons were collected from three South African citrus farms (Addo, Riverside and Mystic), while Lisbon lemons were only collected from two citrus farms (Addo and Riverside) in 2017 and 2018. A total of 36 essential oils were extracted using the Clevenger like apparatus. Analyses of these oils were done using GCMS. An infusion extract of C. limon was prepared according to the standard method and subjected for phytochemical screening to detect and quantify secondary metabolites present. D-Limonene was the major compound identified in all the 36 oils from Eureka and Lisbon peels and leaves (27.9-74.8%). Other dominant compounds identified in Eureka leaf and peel oils were -pinene (5.8-13.2%), -terpinene (4.8-6.7%), geranial (4.0-15.4%), z-citral (6.7-11.7%), sabinene (4.5-6.3%), nerol (4.0-5.4%) and neryl acetate (6.3-9.5%). Some compounds were however present only in some oil such caryophyllene (6.5%) in Mystic dry leaf oil, geraniol (8.0%) in Addo dry leaf oil, as significant compounds. Lisbon leaf and peel essential oils -pinene (4.4- 11.8%), p-cymene (4.2-6.3%), -terpinene (4.4-6.9%), geranial (8.6-17.3%), z-citral (7.1-13.6%), neryl acetate (4.7-9.1%) (5.4-5.9%) were the other prominent peaks identified in the oils. These C. limon essential oils were further tested for insecticidal activities against maize weevils by conducting repellent assay, fumigant assay and contact toxicity; also plant viii powder was used against maize weevils. In general, for all the bioassay, mortality of the insect increased as the contact days with the oil increased. Contact toxicity assays of C. limon peel essential oils were most effective against S. zeamais in which all weevils were dead by day 3 (100% mortality) at 300 μL, while at other concentrations (50, 100, 200 μL) over 50% mortality was observed. The fumigant potential was high with the peel oil with over 50% mortality at 32 and 40 μL in day 4. The oil had moderate repellent effect on the maize weevil. Riverside Eureka dried powder peel was the most effect in all. These results unveil the medicinal potential of South African citrus waste in post-harvest crop protection and good sources of some chemical compounds which are of medicinal value. Furthermore, this study also reveals the economic potential of these wastes in job creation.
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Correlation of Total Phenolic, Flavonoid and Tannin Content of Bryophyllum pinnatum (Lam.) (Crassulaceae) Extract with the Antioxidant and Anticholinesterase Activities
- Elufioye, Taiwo O, Olusola, Damilare M, Oyedeji, Adebola Omowunmi
- Authors: Elufioye, Taiwo O , Olusola, Damilare M , Oyedeji, Adebola Omowunmi
- Date: 2019
- Subjects: Cholinesterase inhibitors , Antioxidants , Bryophyllum pinnatum
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/1536 , vital:37776 , https://doi.org/10.5530/pj.2019.11.158
- Description: Bryophyllum pinnatum is a perennial herb used in traditional medicine against varieties of ailments such as memory disorder. This study quantitatively estimated the total phenolic (TPC), total flavonoid (TFC) and total proautocyanidin (TPA) contents of extract and fractions of B. pinnatum and correlated them with its antioxidant and anticholinesterase activities. Methanolic extract of the dried leaves was partitioned into n-hexane, ethyl acetate and aqueous fractions. Total phenolic, flavonoids and proanthocyanidins content were estimated as gallic acid or quercetin equivalents. DPPH and nitric oxide scavenging activity as well as ferric reducing power assays were used to evaluate antioxidant activity, using 2,6-di-tert-butyl4-methylphenol (DDM) and ascorbic acid as standards. In vitro anticholinesterase activity was evaluated by Ellmann’s colorimetry assay with phsiostigmine (serine) and donepezil as positive control. The crude methanol extract had the highest phenolic, flavonoid and tannin content. The ethyl acetate fraction had the highest DPPH radical scavenging effect (IC50 0.004 mg/ml) while the aqueous fraction had the highest NO scavenging and ferric reducing effects with values of IC50 0.012 mg/ml and 0.007 mg/ml respectively. The ethyl acetate fraction had the best cholinesterase inhibitory effect (IC50 0.951 µg/ml AChE; 3.546 µg/ml BuChE). DPPH radical scavenging effect correlated strongly with total phenolic, flavonoids and proautocyanidins (r2 0.896, 0.651 and 0.619 respectively) while ferric reducing power showed weak correlation and NO scavenging as well as AChE inhibition had no correlation. The study shows DPPH radical scavenging could be due to the phenolic content while other class of compounds are responsible for the cholinesterase inhibition.
- Full Text:
- Authors: Elufioye, Taiwo O , Olusola, Damilare M , Oyedeji, Adebola Omowunmi
- Date: 2019
- Subjects: Cholinesterase inhibitors , Antioxidants , Bryophyllum pinnatum
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/1536 , vital:37776 , https://doi.org/10.5530/pj.2019.11.158
- Description: Bryophyllum pinnatum is a perennial herb used in traditional medicine against varieties of ailments such as memory disorder. This study quantitatively estimated the total phenolic (TPC), total flavonoid (TFC) and total proautocyanidin (TPA) contents of extract and fractions of B. pinnatum and correlated them with its antioxidant and anticholinesterase activities. Methanolic extract of the dried leaves was partitioned into n-hexane, ethyl acetate and aqueous fractions. Total phenolic, flavonoids and proanthocyanidins content were estimated as gallic acid or quercetin equivalents. DPPH and nitric oxide scavenging activity as well as ferric reducing power assays were used to evaluate antioxidant activity, using 2,6-di-tert-butyl4-methylphenol (DDM) and ascorbic acid as standards. In vitro anticholinesterase activity was evaluated by Ellmann’s colorimetry assay with phsiostigmine (serine) and donepezil as positive control. The crude methanol extract had the highest phenolic, flavonoid and tannin content. The ethyl acetate fraction had the highest DPPH radical scavenging effect (IC50 0.004 mg/ml) while the aqueous fraction had the highest NO scavenging and ferric reducing effects with values of IC50 0.012 mg/ml and 0.007 mg/ml respectively. The ethyl acetate fraction had the best cholinesterase inhibitory effect (IC50 0.951 µg/ml AChE; 3.546 µg/ml BuChE). DPPH radical scavenging effect correlated strongly with total phenolic, flavonoids and proautocyanidins (r2 0.896, 0.651 and 0.619 respectively) while ferric reducing power showed weak correlation and NO scavenging as well as AChE inhibition had no correlation. The study shows DPPH radical scavenging could be due to the phenolic content while other class of compounds are responsible for the cholinesterase inhibition.
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Evaluating the Prevention of Mother to Child Transmission (PMTCT) of HIV Services in the Eastern Cape: subtitle if needed. If no subtitle follow instructions in manual
- Authors: Sigovana, Khuthala
- Date: 2019
- Language: English
- Type: Masters theses , text
- Identifier: http://hdl.handle.net/11260/3176 , vital:43182
- Description: Abstract text To effectively reduce HIV transmission to newborns requires a reduction of HIV prevalence among pregnant women and a full understanding of its epidemiology. There is however, a literature gap: few recent HIV studies focus on women attending antenatal care in rural areas in South Africa. A Cross-sectional study of women attending antenatal care in four Primary Care facilities was conducted. An interviewer-administered questionnaire to collect information on socio-demographic characteristics and medical history was used. Binomial logistic regression analyses were used to determine factors associated with HIV and to estimate the prevalence ratio (PR). The 95% confidence interval (CI) is used for precision of estimates; p≤0.05 for statistical significance. A total of 343 participants were recruited. Most participants (n = 341 or 99.42%; CI: 97.91 – 99.93) knew their HIV status. The antenatal HIV prevalence was 38.19% (CI: 33.17 – 43.48). Participants older than 40 years were 3.99 and 3.81 times more likely to be HIV positive compared to teenagers (PR = 3.99; CI: 1.11 – 14.30; pstxvalue = 0.034) and those in the 20-29 age group (PR = 3.81; CI: 1.07 – 13.60; pstxvaluevalue = 0.039) respectively; and this was statistically significant. Unemployed participants were 34% (PR = 1.34; CI: 1.16 – 1.55; p-value less than 0.0001) and 41 percent (PR = 1.41; CI: 1.23 – 1.61; p-value less than 0.0001) more likely to be HIV positive when compared to tertiary students and self-employed individuals respectively. The antenatal HIV prevalence remains high and is increasing in this population. However, findings suggest a changing and maturing epidemic. , Thesis (MMED) -- Faculty of Health Sciences, 2019
- Full Text:
- Authors: Sigovana, Khuthala
- Date: 2019
- Language: English
- Type: Masters theses , text
- Identifier: http://hdl.handle.net/11260/3176 , vital:43182
- Description: Abstract text To effectively reduce HIV transmission to newborns requires a reduction of HIV prevalence among pregnant women and a full understanding of its epidemiology. There is however, a literature gap: few recent HIV studies focus on women attending antenatal care in rural areas in South Africa. A Cross-sectional study of women attending antenatal care in four Primary Care facilities was conducted. An interviewer-administered questionnaire to collect information on socio-demographic characteristics and medical history was used. Binomial logistic regression analyses were used to determine factors associated with HIV and to estimate the prevalence ratio (PR). The 95% confidence interval (CI) is used for precision of estimates; p≤0.05 for statistical significance. A total of 343 participants were recruited. Most participants (n = 341 or 99.42%; CI: 97.91 – 99.93) knew their HIV status. The antenatal HIV prevalence was 38.19% (CI: 33.17 – 43.48). Participants older than 40 years were 3.99 and 3.81 times more likely to be HIV positive compared to teenagers (PR = 3.99; CI: 1.11 – 14.30; pstxvalue = 0.034) and those in the 20-29 age group (PR = 3.81; CI: 1.07 – 13.60; pstxvaluevalue = 0.039) respectively; and this was statistically significant. Unemployed participants were 34% (PR = 1.34; CI: 1.16 – 1.55; p-value less than 0.0001) and 41 percent (PR = 1.41; CI: 1.23 – 1.61; p-value less than 0.0001) more likely to be HIV positive when compared to tertiary students and self-employed individuals respectively. The antenatal HIV prevalence remains high and is increasing in this population. However, findings suggest a changing and maturing epidemic. , Thesis (MMED) -- Faculty of Health Sciences, 2019
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Exploring Teacher Assessment Practices in The General Education and Training Level in Junior Secondary Schools in Mthatha Education District
- Authors: Novukela, Cawe Sandys
- Date: 2019
- Language: English
- Type: Masters theses , text
- Identifier: http://hdl.handle.net/11260/3460 , vital:43409
- Description: Diabetes mellitus is one of the largest and most important public health emergencies of our time that has resulted in human suffering and huge financial implications for organisations, governments and individuals (IDF Atlas, 2015). Furthermore there is growing evidence that a number of employees are overstretched in their work environments and this has resulted in stress and burnout (McCormack and Cotter, 2013). This study investigated the effects of diabetes mellitus on burnout among WSU employees as well as the prevalence of diabetes mellitus and burnout. A mixed method research design using quantitative and qualitative approaches was employed in order to explore the effects of diabetes on burnout as well as the prevalence of diabetes and burnout at WSU. A cross-sectional survey was used to gather quantitative data and semi-structured interviews were used for the qualitative data collection. A total number of 169 participants were involved in this study. This included 154 participants and 15 participants who were selected for the quantitative and qualitative respectively using random sampling technique and purposive sampling method. The quantitative data was analysed using Stata version 13. The study findings indicated that the prevalence of diabetes at WSU was 16% and 57% participants were burned out. It was further found that the majority of participants (58%) who were diabetic also suffered from burnout. The relationship between diabetes and burnout was found to be significant. Qualitative data analysis involved coding data and analysing the emerging themes to form the basis of the findings. The results of the study found an emerging problem of high level of burnout and also a significant number of employees who were diabetic. A combination of these two conditions poses a challenge to the continued uninterrupted functioning of WSU. It is recommended that the university should educate its employees about diabetes and burnout as well as create an environment that promotes healthy lifestyle. A model has been developed that could help deal with diabetes and burnout. , Thesis - Faculty of Health Sciences, 2019
- Full Text:
- Authors: Novukela, Cawe Sandys
- Date: 2019
- Language: English
- Type: Masters theses , text
- Identifier: http://hdl.handle.net/11260/3460 , vital:43409
- Description: Diabetes mellitus is one of the largest and most important public health emergencies of our time that has resulted in human suffering and huge financial implications for organisations, governments and individuals (IDF Atlas, 2015). Furthermore there is growing evidence that a number of employees are overstretched in their work environments and this has resulted in stress and burnout (McCormack and Cotter, 2013). This study investigated the effects of diabetes mellitus on burnout among WSU employees as well as the prevalence of diabetes mellitus and burnout. A mixed method research design using quantitative and qualitative approaches was employed in order to explore the effects of diabetes on burnout as well as the prevalence of diabetes and burnout at WSU. A cross-sectional survey was used to gather quantitative data and semi-structured interviews were used for the qualitative data collection. A total number of 169 participants were involved in this study. This included 154 participants and 15 participants who were selected for the quantitative and qualitative respectively using random sampling technique and purposive sampling method. The quantitative data was analysed using Stata version 13. The study findings indicated that the prevalence of diabetes at WSU was 16% and 57% participants were burned out. It was further found that the majority of participants (58%) who were diabetic also suffered from burnout. The relationship between diabetes and burnout was found to be significant. Qualitative data analysis involved coding data and analysing the emerging themes to form the basis of the findings. The results of the study found an emerging problem of high level of burnout and also a significant number of employees who were diabetic. A combination of these two conditions poses a challenge to the continued uninterrupted functioning of WSU. It is recommended that the university should educate its employees about diabetes and burnout as well as create an environment that promotes healthy lifestyle. A model has been developed that could help deal with diabetes and burnout. , Thesis - Faculty of Health Sciences, 2019
- Full Text:
Factors associated with consistent condom use: a cross-sectional survey of two Nigerian universities
- Ajayi, Anthony Idowu, Ismail , Kafayat Olanike, Akpan, Wilson
- Authors: Ajayi, Anthony Idowu , Ismail , Kafayat Olanike , Akpan, Wilson
- Date: 2019
- Subjects: Nigeria Condom Use Computer File
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/5834 , vital:44650 , https://DOI:10.1186/s12889-019-7543-1
- Description: Background: Consistent condom use is central to the prevention of transmission of human immunodeficiency virus (HIV) and other sexually transmitted diseases (STDs), especially among young adults. This study drew from a cross-sectional survey of two Nigerian universities to determine the level of consistent condom use, explored the determinants of condom use consistency and reasons for inconsistent condom use. Methods: We adopted a descriptive cross-sectional design, which involves the recruitment of 800 male and female students using stratified random sampling. Adjusted and unadjusted logistic regression models were used to examine the factors associated with consistent condom use among a final sample of 498 students who engaged in sex in the last year preceding the study. Results: Only 38.6% of sexually active participants (n = 498) used condoms consistently in the previous year. High condom self-efficacy score (AOR: 2.40; 95% CI: 1.58–3.64), discussion of HIV/STIs with sexual partner (AOR: 1.91; 95%CI: 1.29–2.83), knowing partner’s HIV status (AOR: 1.48; 95% CI: 1.02–2.16), being students of university located in a high HIV prevalence area (AOR: 2.86; 95% CI: 1.92–4.28) and engaging in sex with only steady partner (AOR: 1.74; 95% CI: 1.17–2.60) were associated with a higher odds of consistent condom use. Trust, unavailability of condoms, dislike of condoms and a perception that condoms reduced sexual pleasure were the main reasons for inconsistent use of condoms. Conclusion: The study found a low level of consistent condom use among study participants. Counselling young adults in Nigeria on condom self-efficacy, providing condoms on campuses and encouraging the discussion of sexually transmitted infections with sexual partners are central to improving the level of consistent condom use among Nigerian university students. Keywords: Condoms, Self-efficacy, Consistent condom use, HIV, Drug use, Alcohol use, Nigeria, University students
- Full Text:
Factors associated with consistent condom use: a cross-sectional survey of two Nigerian universities
- Authors: Ajayi, Anthony Idowu , Ismail , Kafayat Olanike , Akpan, Wilson
- Date: 2019
- Subjects: Nigeria Condom Use Computer File
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/5834 , vital:44650 , https://DOI:10.1186/s12889-019-7543-1
- Description: Background: Consistent condom use is central to the prevention of transmission of human immunodeficiency virus (HIV) and other sexually transmitted diseases (STDs), especially among young adults. This study drew from a cross-sectional survey of two Nigerian universities to determine the level of consistent condom use, explored the determinants of condom use consistency and reasons for inconsistent condom use. Methods: We adopted a descriptive cross-sectional design, which involves the recruitment of 800 male and female students using stratified random sampling. Adjusted and unadjusted logistic regression models were used to examine the factors associated with consistent condom use among a final sample of 498 students who engaged in sex in the last year preceding the study. Results: Only 38.6% of sexually active participants (n = 498) used condoms consistently in the previous year. High condom self-efficacy score (AOR: 2.40; 95% CI: 1.58–3.64), discussion of HIV/STIs with sexual partner (AOR: 1.91; 95%CI: 1.29–2.83), knowing partner’s HIV status (AOR: 1.48; 95% CI: 1.02–2.16), being students of university located in a high HIV prevalence area (AOR: 2.86; 95% CI: 1.92–4.28) and engaging in sex with only steady partner (AOR: 1.74; 95% CI: 1.17–2.60) were associated with a higher odds of consistent condom use. Trust, unavailability of condoms, dislike of condoms and a perception that condoms reduced sexual pleasure were the main reasons for inconsistent use of condoms. Conclusion: The study found a low level of consistent condom use among study participants. Counselling young adults in Nigeria on condom self-efficacy, providing condoms on campuses and encouraging the discussion of sexually transmitted infections with sexual partners are central to improving the level of consistent condom use among Nigerian university students. Keywords: Condoms, Self-efficacy, Consistent condom use, HIV, Drug use, Alcohol use, Nigeria, University students
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Factors influencing English reading competencies of Grade 4 learners in the Libode-Mega District of the Eastern Cape Province of South Africa
- Authors: Nomkopo, Cornas Ngephi
- Date: 2019
- Subjects: English reading competency -- School learners Reading(Primary) -- Eastern Cape
- Language: English
- Type: Thesis , Masters/Doctoral , M Ed
- Identifier: http://hdl.handle.net/11260/1400 , vital:34371
- Description: Reading has become the national and international centre of debate as learners persistently read below the required levels and matriculation results are due to learners‟ poor reading competencies. The concerns about poor reading competencies has mounted into inflammatory statements in articles and popular media nationally, continentally and internationally. Without the ability to read learners are deprived access to pertinent information and knowledge that can assist individuals in lifelong learning. Being a language teacher, the researcher in this study strongly believe that every learner can read appropriately and teachers can assist learners to improve their reading competencies. The purpose of the study was to identify the factors contributing to poor reading competencies of grade 4 learners and to provide recommendations to assist in dealing with poor reading competencies. Qualitative research approach was undertaken to deal with all aspects related to research methodology. The data were collected using research instruments such as observation of six learners from three selected primary schools. The observation was conducted during reading of text by learners in the presence of English teachers. The questionnaires were distributed to eleven teachers offering English in grade 4 as another means of data collection instrument. Major findings of the study revealed that factors affecting reading competencies are hugely emanated from schools, teachers, family background, departmental policy and learners themselves. Despite reading problems exist in schools, there are always positive solutions to bring hope and success to the learners.
- Full Text:
- Authors: Nomkopo, Cornas Ngephi
- Date: 2019
- Subjects: English reading competency -- School learners Reading(Primary) -- Eastern Cape
- Language: English
- Type: Thesis , Masters/Doctoral , M Ed
- Identifier: http://hdl.handle.net/11260/1400 , vital:34371
- Description: Reading has become the national and international centre of debate as learners persistently read below the required levels and matriculation results are due to learners‟ poor reading competencies. The concerns about poor reading competencies has mounted into inflammatory statements in articles and popular media nationally, continentally and internationally. Without the ability to read learners are deprived access to pertinent information and knowledge that can assist individuals in lifelong learning. Being a language teacher, the researcher in this study strongly believe that every learner can read appropriately and teachers can assist learners to improve their reading competencies. The purpose of the study was to identify the factors contributing to poor reading competencies of grade 4 learners and to provide recommendations to assist in dealing with poor reading competencies. Qualitative research approach was undertaken to deal with all aspects related to research methodology. The data were collected using research instruments such as observation of six learners from three selected primary schools. The observation was conducted during reading of text by learners in the presence of English teachers. The questionnaires were distributed to eleven teachers offering English in grade 4 as another means of data collection instrument. Major findings of the study revealed that factors affecting reading competencies are hugely emanated from schools, teachers, family background, departmental policy and learners themselves. Despite reading problems exist in schools, there are always positive solutions to bring hope and success to the learners.
- Full Text:
Gender stereotyping in traditional healing practices
- Authors: Mayaba, Nothemba
- Date: 2019
- Subjects: Gender identity -- Traditional healing -- Education Traditional medicines -- Primary health care Female traditional healers - - South Africa – Eastern
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/11260/1354 , vital:32262
- Description: People residing in rural areas use traditional medicines as their primary health care. They consult traditional healers. Traditional healing is a gender-based practice, although both men and women engaged in this practice. In some communities there are more female traditional healers than male traditional healers, including Mhlontlo Local Municipality. The main aim of the study was to investigate gender stereotyping in traditional healing in Mhlontlo Local Municipality. This study also investigated the relationship between male and female traditional healers, challenges faced by female traditional healers, transfer of traditional knowledge by female traditional healers and traditional healers’ unions. Snowball sampling was used to identify and select 79 traditional healers from Mhlontlo Local Municipality. Data was gathered using a questionnaire. The majority of traditional healers in the study area were females. Most of them have primary education, some with secondary education, and others with no formal education, and few with tertiary school. Most traditional healers were between 46-60 years. Female traditional healers constitute 70% of the study. The majority of them see between 11 and 20 patients per month. Most of the traditional healers had an experience of 21-30 years in traditional healing. The study also revealed that female traditional healers experience challenges in traditional healing. Diviners and herbalists work together, this denotes a relationship between them. Female traditional healers also have a problem in transferring their knowledge and registering under traditional healers’ unions. In conclusion, the study found out that traditional healing is gender stereotyped. There are more diviners than herbalists, where most diviners are females and herbalists are males. There is no transfer of knowledge among diviners, as being a diviner is an ancestral calling.
- Full Text:
- Authors: Mayaba, Nothemba
- Date: 2019
- Subjects: Gender identity -- Traditional healing -- Education Traditional medicines -- Primary health care Female traditional healers - - South Africa – Eastern
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/11260/1354 , vital:32262
- Description: People residing in rural areas use traditional medicines as their primary health care. They consult traditional healers. Traditional healing is a gender-based practice, although both men and women engaged in this practice. In some communities there are more female traditional healers than male traditional healers, including Mhlontlo Local Municipality. The main aim of the study was to investigate gender stereotyping in traditional healing in Mhlontlo Local Municipality. This study also investigated the relationship between male and female traditional healers, challenges faced by female traditional healers, transfer of traditional knowledge by female traditional healers and traditional healers’ unions. Snowball sampling was used to identify and select 79 traditional healers from Mhlontlo Local Municipality. Data was gathered using a questionnaire. The majority of traditional healers in the study area were females. Most of them have primary education, some with secondary education, and others with no formal education, and few with tertiary school. Most traditional healers were between 46-60 years. Female traditional healers constitute 70% of the study. The majority of them see between 11 and 20 patients per month. Most of the traditional healers had an experience of 21-30 years in traditional healing. The study also revealed that female traditional healers experience challenges in traditional healing. Diviners and herbalists work together, this denotes a relationship between them. Female traditional healers also have a problem in transferring their knowledge and registering under traditional healers’ unions. In conclusion, the study found out that traditional healing is gender stereotyped. There are more diviners than herbalists, where most diviners are females and herbalists are males. There is no transfer of knowledge among diviners, as being a diviner is an ancestral calling.
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Improving intravenous fluid prescribing in the Eastern Cape in South Africa
- Luce, Cate, Soffair,Rosie, Parrish, Andy
- Authors: Luce, Cate , Soffair,Rosie , Parrish, Andy
- Date: 2019
- Subjects: South Africa Intravenous fluids Computer File
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/5120 , vital:44351 , https://doi:10.1136/bmjoq-2018-000406
- Description: Intravenous fluids are an essential component of patient care, but a 2013 National Institute for Health and Care Excellence (NICE) guideline noted that inappropriate prescribing or administration may lead to actual or potential patient harm in up to 20% of patients.1 This project aimed to improve prescribing documentation and communication between nurses and doctors regarding intravenous fluid management. This was done through the introduction of a fluid chart, in combination with teaching on appropriate fluid management. It was initiated within the medical department of Cecelia Makiwane Hospital in East London, South Africa. 309 patients were included and data were analysed over a 6-month period. The outcome measures were the standards of intravenous fluid prescribing set by the NICE guidelines. The process measure was the use of the new chart. Baseline data highlighted that there was no standardised location for fluid prescriptions within the bedside notes. Following the intervention, 81% of fluid prescriptions were on a fluid chart. The percentage of fluid scripts with a 24-hour fluid prescription, a recorded indication and recorded input increased after the intervention. Seventy six per cent of patients received more than 50% of the fluids prescribed following the intervention compared with 22% at baseline. These results indicated an increase in the doctor’s awareness of appropriate fluid prescribing and an improvement in the communication between doctors and nurses regarding the patient’s fluid management. The engagement of local stakeholders and staff was fundamental to the success of the project and allowed for this change in practice. Fluid management is a vital part of care in many specialties; therefore, a toolkit has been created to allow similar quality improvement projects to be implemented across other hospitals around the Eastern Cape.
- Full Text:
- Authors: Luce, Cate , Soffair,Rosie , Parrish, Andy
- Date: 2019
- Subjects: South Africa Intravenous fluids Computer File
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/5120 , vital:44351 , https://doi:10.1136/bmjoq-2018-000406
- Description: Intravenous fluids are an essential component of patient care, but a 2013 National Institute for Health and Care Excellence (NICE) guideline noted that inappropriate prescribing or administration may lead to actual or potential patient harm in up to 20% of patients.1 This project aimed to improve prescribing documentation and communication between nurses and doctors regarding intravenous fluid management. This was done through the introduction of a fluid chart, in combination with teaching on appropriate fluid management. It was initiated within the medical department of Cecelia Makiwane Hospital in East London, South Africa. 309 patients were included and data were analysed over a 6-month period. The outcome measures were the standards of intravenous fluid prescribing set by the NICE guidelines. The process measure was the use of the new chart. Baseline data highlighted that there was no standardised location for fluid prescriptions within the bedside notes. Following the intervention, 81% of fluid prescriptions were on a fluid chart. The percentage of fluid scripts with a 24-hour fluid prescription, a recorded indication and recorded input increased after the intervention. Seventy six per cent of patients received more than 50% of the fluids prescribed following the intervention compared with 22% at baseline. These results indicated an increase in the doctor’s awareness of appropriate fluid prescribing and an improvement in the communication between doctors and nurses regarding the patient’s fluid management. The engagement of local stakeholders and staff was fundamental to the success of the project and allowed for this change in practice. Fluid management is a vital part of care in many specialties; therefore, a toolkit has been created to allow similar quality improvement projects to be implemented across other hospitals around the Eastern Cape.
- Full Text:
Improving TB infection control in a regional hospital in the Eastern Cape, South Africa
- Haeusler, Ilsa Louisa, Knights, Felicity, George, Vishaal, Parrish, Andy
- Authors: Haeusler, Ilsa Louisa , Knights, Felicity , George, Vishaal , Parrish, Andy
- Date: 2019
- Subjects: Quality circles Tuberculosis--Prevention Computer File
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/6399 , vital:45465 , https://doi.org/10.4102/hts.v77i2.6828
- Description: This quality improvement (QI) work was carried out in Cecilia Makiwane Hospital (CMH), a regional public hospital in the Eastern Cape, South Africa (SA). SA has among the highest incidence of tuberculosis (TB) in the world and this is a leading cause of death in SA. Nosocomial infection is an important source of TB transmission. Adherence to TB infection prevention control (IPC) measures in the medical inpatient department was suboptimal at CMH. The overall aim of this QI project was to make sustainable improvements in TB IPC. A multidisciplinary team was formed to undertake a root cause analysis and develop a strategy for change. The main barriers to adherence to IPC measures were limited knowledge of IPC methods and stigma associated with TB. Specifically, the project aimed to increase the number of: ‘airborne precaution’ signs placed above patients’ beds, patients correctly isolated and patients wearing surgical face masks. Four Plan Do-Study-Act cycles were used. The strategy for change involved education and awareness-raising in different formats, including formal in-service training delivered to nurses and doctors, a hospital-wide TB awareness week with engaging activities and competitions, and a World TB Day provincial solidarity march. Data on adherence to the three IPC measures were collected over an 8-month period. Pre-intervention (October 2016), a mean of 2 percent of patients wore face masks, 22 percent were correctly isolated and 12 percent had an airborne precaution sign. Postintervention (May 2017), the compliance improved to 17 percent, 50 percent and 25 percent, respectively. There was a large variation in compliance to each measure. Improvement was greatest in the number of patients correctly isolated. We learnt it is important to work with, not in parallel to, existing teams or structures during QI work. On-the-ground training of nurses and clinicians should be undertaken alongside engagement of senior staff members and managers. This improves the chance of change being adopted into hospital policy.
- Full Text:
- Authors: Haeusler, Ilsa Louisa , Knights, Felicity , George, Vishaal , Parrish, Andy
- Date: 2019
- Subjects: Quality circles Tuberculosis--Prevention Computer File
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/6399 , vital:45465 , https://doi.org/10.4102/hts.v77i2.6828
- Description: This quality improvement (QI) work was carried out in Cecilia Makiwane Hospital (CMH), a regional public hospital in the Eastern Cape, South Africa (SA). SA has among the highest incidence of tuberculosis (TB) in the world and this is a leading cause of death in SA. Nosocomial infection is an important source of TB transmission. Adherence to TB infection prevention control (IPC) measures in the medical inpatient department was suboptimal at CMH. The overall aim of this QI project was to make sustainable improvements in TB IPC. A multidisciplinary team was formed to undertake a root cause analysis and develop a strategy for change. The main barriers to adherence to IPC measures were limited knowledge of IPC methods and stigma associated with TB. Specifically, the project aimed to increase the number of: ‘airborne precaution’ signs placed above patients’ beds, patients correctly isolated and patients wearing surgical face masks. Four Plan Do-Study-Act cycles were used. The strategy for change involved education and awareness-raising in different formats, including formal in-service training delivered to nurses and doctors, a hospital-wide TB awareness week with engaging activities and competitions, and a World TB Day provincial solidarity march. Data on adherence to the three IPC measures were collected over an 8-month period. Pre-intervention (October 2016), a mean of 2 percent of patients wore face masks, 22 percent were correctly isolated and 12 percent had an airborne precaution sign. Postintervention (May 2017), the compliance improved to 17 percent, 50 percent and 25 percent, respectively. There was a large variation in compliance to each measure. Improvement was greatest in the number of patients correctly isolated. We learnt it is important to work with, not in parallel to, existing teams or structures during QI work. On-the-ground training of nurses and clinicians should be undertaken alongside engagement of senior staff members and managers. This improves the chance of change being adopted into hospital policy.
- Full Text:
Management Capacity within Small to Medium Tourism Enterprises (SMTEs) in the Eastern Cape Province
- Mxunyelwa, Siyabonga, Lloyd, Hendrik
- Authors: Mxunyelwa, Siyabonga , Lloyd, Hendrik
- Date: 2019
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/1962 , vital:39807 , https://www.ajhtl.com/uploads/7/1/6/3/7163688/article_49_vol_8_4__2019_wsu.pdf
- Description: The small to medium tourism enterprises (SMTEs) are recognised as significant contributors to the development of the local and regional economies and create much needed entrepreneurial ventures. Universally, SMTEs play a significant role in the tourism sector. Management of these enterprises by owners and managers seems to face challenges. This paper examines the factors impacting on management capacity with specific focus on the SMTEs in the Eastern Cape Province, South Africa. A systematic random sampling technique was utilised to choose a sample of 320 business respondents from the 2012 database of the Eastern Cape Parks and Tourism Agency, comprising of formally registered small tourism businesses. A total of 310 usable questionnaires was finally obtained. In this study descriptive and inferential statistics were applied. Descriptive statistics were captured to highlight the owners’ characteristics and to consolidate their measurement scores. Utilising inferential statistics, the study undertook an investigation into relationships between demographic variables, business characteristics and factors impacting on management capacity within SMTEs were undertaken. The paper elucidates that a significant relationship was found between business characteristics and demographic variables. Furthermore, skills development, strategic and management knowledge and business leadership factors were compared with the qualifications group. This study adds to the body of knowledge in a predominantly less researched phenomenon of management capacity within SMTEs.
- Full Text:
- Authors: Mxunyelwa, Siyabonga , Lloyd, Hendrik
- Date: 2019
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/1962 , vital:39807 , https://www.ajhtl.com/uploads/7/1/6/3/7163688/article_49_vol_8_4__2019_wsu.pdf
- Description: The small to medium tourism enterprises (SMTEs) are recognised as significant contributors to the development of the local and regional economies and create much needed entrepreneurial ventures. Universally, SMTEs play a significant role in the tourism sector. Management of these enterprises by owners and managers seems to face challenges. This paper examines the factors impacting on management capacity with specific focus on the SMTEs in the Eastern Cape Province, South Africa. A systematic random sampling technique was utilised to choose a sample of 320 business respondents from the 2012 database of the Eastern Cape Parks and Tourism Agency, comprising of formally registered small tourism businesses. A total of 310 usable questionnaires was finally obtained. In this study descriptive and inferential statistics were applied. Descriptive statistics were captured to highlight the owners’ characteristics and to consolidate their measurement scores. Utilising inferential statistics, the study undertook an investigation into relationships between demographic variables, business characteristics and factors impacting on management capacity within SMTEs were undertaken. The paper elucidates that a significant relationship was found between business characteristics and demographic variables. Furthermore, skills development, strategic and management knowledge and business leadership factors were compared with the qualifications group. This study adds to the body of knowledge in a predominantly less researched phenomenon of management capacity within SMTEs.
- Full Text:
On-site evaluation of smoking, alcohol consumption and physical inactivity among commercial taxi drivers in Buffalo City Metropolitan Municipality, South Africa
- Adedokun, Aanuoluwa Odunayo, Ter Goon, Daniel, Owolabi, Eyitayo Omolara, Adeniyi, Oladele Vincent, Ajayi, Anthony Idowu
- Authors: Adedokun, Aanuoluwa Odunayo , Ter Goon, Daniel , Owolabi, Eyitayo Omolara , Adeniyi, Oladele Vincent , Ajayi, Anthony Idowu
- Date: 2019
- Subjects: Alcohol use , Substance use , Commercial drivers
- Language: English
- Type: article , text
- Identifier: http://hdl.handle.net/11260/5086 , vital:44345 , https://doi.org/10.5539/gjhs.v11n2p110
- Description: Commercial drivers have been identified as eliciting behaviours that promote non- communicable diseases and road traffic accidents. The aim of the study is to determine the prevalence and pattern of alcohol use, smoking and physical inactivity among commercial taxi drivers in Buffalo City Metropolitan Municipality, South Africa. A cross-sectional study was conducted among 403 commercial drivers using the face-to-face interviews method. The WHO STEPwise questionnaire was used to obtain the demographic data, self-reported rate of alcohol consumption, tobacco use and physical inactivity. The participants’ mean age was 43.3 ± 12.5 years. About 30% of the participants were daily smokers, 37% consumed alcohol regularly and only 18% were physically active, whilst 82% were physically inactive. The prevalence of alcohol use, smoking and physical inactivity is high among commercial drivers in East London. Workplace health education on the health effects of these lifestyles’ risky behaviours on individuals and the general public should be given to the drivers.
- Full Text:
- Authors: Adedokun, Aanuoluwa Odunayo , Ter Goon, Daniel , Owolabi, Eyitayo Omolara , Adeniyi, Oladele Vincent , Ajayi, Anthony Idowu
- Date: 2019
- Subjects: Alcohol use , Substance use , Commercial drivers
- Language: English
- Type: article , text
- Identifier: http://hdl.handle.net/11260/5086 , vital:44345 , https://doi.org/10.5539/gjhs.v11n2p110
- Description: Commercial drivers have been identified as eliciting behaviours that promote non- communicable diseases and road traffic accidents. The aim of the study is to determine the prevalence and pattern of alcohol use, smoking and physical inactivity among commercial taxi drivers in Buffalo City Metropolitan Municipality, South Africa. A cross-sectional study was conducted among 403 commercial drivers using the face-to-face interviews method. The WHO STEPwise questionnaire was used to obtain the demographic data, self-reported rate of alcohol consumption, tobacco use and physical inactivity. The participants’ mean age was 43.3 ± 12.5 years. About 30% of the participants were daily smokers, 37% consumed alcohol regularly and only 18% were physically active, whilst 82% were physically inactive. The prevalence of alcohol use, smoking and physical inactivity is high among commercial drivers in East London. Workplace health education on the health effects of these lifestyles’ risky behaviours on individuals and the general public should be given to the drivers.
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Phytochemical Analysis and Biological potential of leaves and peels of three Eastern Cape Citrus Species
- Authors: Matuka, Thandiswa
- Date: 2019
- Subjects: Phytochemical -- Analysis Biology -- Leaves and peels Citrus species -- Eastern Cape -- South Africa
- Language: English
- Type: Thesis , Masters , M Sc
- Identifier: http://hdl.handle.net/11260/1362 , vital:32263
- Description: Citrus sinensis, Citrus reticulata and Citrus clementine are citrus species that belong to a large family Rutaceae. Citrus leaves and peels are often regarded as waste products. In view of the environment pollution impact, the search for end use of citrus waste products for economic value and medicinal purposes was sort after. The aim of this study was to investigate chemical composition of citrus waste products (leaves and peels) essential oils and determine biological active volatile compounds from Citrus sinensis, Citrus reticulata and Citrus clementine Leaves and peels of Citrus sinensis, Citrus reticulata and Citrus clementine collected twice from citrus farm were extracted for essential oils using hydrodistillation method and analysed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) for plant samples collected in 2016 and, gas chromatography (GC) and gas chromatography-triple quadrupole-mass spectrometry (GC-MSMS) for plant samples collected in 2017. The oil yield for Citrus sinensis was 0.19 - 0.85 %v/w for fresh and dried leaf and peels for samples collected in 2016 and 0.26 – 0.87% v/w for those collected in 2017. Analysis of the essential oils analyses identified sabinene (20.4% GCMS, 22.6% GCMSMS), terpinen-4-ol (13.2% GCMS, 6.8% GCMSMS) and linalool (7.6% GCMS, 19.3% GCMSMS) as major compounds in fresh leaves essential oils while β-elemene (16.3% GCMS, 21.4% GCMSMS) and sabinene (10.7% GCMS, 20.8% GCMSMS) were major compounds characterized in dried leaves essential oils. Fresh and dried peels essential oils had limonene as the major constituent (49.6% GCMS, 87.1% GCMSMS) and (73.6% GCMS, 88.3% GCMSMS) respectively. Citrus reticulata essential oils percentage yield range between 0.44-0.80% v/w for fresh and dried leaf and peel oils. Analyses of the GCMS and GCMSMS chromatogram of the volatile oils analyses revealed linalool (33.4% GCMS, 19.6% GCMSMS), (+)-spathulenol (15.2% GCMS, 10.1% GCMSMS) and terpinene-4-ol (4.5% GCMS, 11.9% GCMSMS) as major compounds in fresh leaf essential oils while, sabinene (20.8% GCMS, 32.5% GCMSMS) and linalool (17.2% GCMS, 20.4% GCMSMS) were the major compounds found in dried leaf essential oils. Limonene (55.2%, 75.4% GCMS; 39.5%, 80.8% GCMSMS) was the major prominent compound identified in the C. reticulata peel essential oils in fresh and dried peels respectively, followed by linalool (12.8%, 2.9% GCMS; 48.4%, 7.4% GCMSMS). Percentage yields for the essential oils from C. clementine fresh and dried leaves and peels ranges from 0.40-0.62% v/w for. Fresh and dried leaves volatile oils had sabinene (22.7-27.1%) for GCMS and GCMSMS respectively), linalool (18.5- 20.9% GCMS and GCMSMS) and terpinene-4-ol (6.5- 11.8% GCMS and GCMSMS) as main compounds while fresh and dried peels oils were dominated by limonene (38.9- 86.5% GCMS and GCMSMS). Phytochemical screening experiment of the three citrus species leaves and peels aqueous extract reveal the prominent presence of flavonoids and phenolic compounds in high concentrations. C. reticulata and C. clementine peels and leaves volatile oils exhibited higher antioxidant activity as DPPH free radical scavengers and Ferric reducing power (FRAP) ability, when compared to C. sinensis essential oils which exhibited minimum antioxidant activity. Antioxidant activity of leaves and peels essential oils maybe due to the presence of oxygenated monoterpenes and high limonene content. C. sinensis peels oils showed non-toxic to extremely very low toxicity effect at highest dose 2000 mg/kg. C. reticulata and C. clementine peels oils showed toxicity effect in mice at high dose levels. It is suggested that citrus volatile oils could be safe to use at lower dose levels. Citrus species peels and leaves volatile compounds limonene linalool and sabinene exhibited antiinflammatory activity by significantly reducing rats paw oedema size induced by fresh egg albumin. The study suggests the use of Citrus waste products (leaves and peels) as natural antioxidants with antiinflammatory properties for medicinal value and volatile oils compounds for aroma in beverages and cosmetic industries.
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- Authors: Matuka, Thandiswa
- Date: 2019
- Subjects: Phytochemical -- Analysis Biology -- Leaves and peels Citrus species -- Eastern Cape -- South Africa
- Language: English
- Type: Thesis , Masters , M Sc
- Identifier: http://hdl.handle.net/11260/1362 , vital:32263
- Description: Citrus sinensis, Citrus reticulata and Citrus clementine are citrus species that belong to a large family Rutaceae. Citrus leaves and peels are often regarded as waste products. In view of the environment pollution impact, the search for end use of citrus waste products for economic value and medicinal purposes was sort after. The aim of this study was to investigate chemical composition of citrus waste products (leaves and peels) essential oils and determine biological active volatile compounds from Citrus sinensis, Citrus reticulata and Citrus clementine Leaves and peels of Citrus sinensis, Citrus reticulata and Citrus clementine collected twice from citrus farm were extracted for essential oils using hydrodistillation method and analysed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) for plant samples collected in 2016 and, gas chromatography (GC) and gas chromatography-triple quadrupole-mass spectrometry (GC-MSMS) for plant samples collected in 2017. The oil yield for Citrus sinensis was 0.19 - 0.85 %v/w for fresh and dried leaf and peels for samples collected in 2016 and 0.26 – 0.87% v/w for those collected in 2017. Analysis of the essential oils analyses identified sabinene (20.4% GCMS, 22.6% GCMSMS), terpinen-4-ol (13.2% GCMS, 6.8% GCMSMS) and linalool (7.6% GCMS, 19.3% GCMSMS) as major compounds in fresh leaves essential oils while β-elemene (16.3% GCMS, 21.4% GCMSMS) and sabinene (10.7% GCMS, 20.8% GCMSMS) were major compounds characterized in dried leaves essential oils. Fresh and dried peels essential oils had limonene as the major constituent (49.6% GCMS, 87.1% GCMSMS) and (73.6% GCMS, 88.3% GCMSMS) respectively. Citrus reticulata essential oils percentage yield range between 0.44-0.80% v/w for fresh and dried leaf and peel oils. Analyses of the GCMS and GCMSMS chromatogram of the volatile oils analyses revealed linalool (33.4% GCMS, 19.6% GCMSMS), (+)-spathulenol (15.2% GCMS, 10.1% GCMSMS) and terpinene-4-ol (4.5% GCMS, 11.9% GCMSMS) as major compounds in fresh leaf essential oils while, sabinene (20.8% GCMS, 32.5% GCMSMS) and linalool (17.2% GCMS, 20.4% GCMSMS) were the major compounds found in dried leaf essential oils. Limonene (55.2%, 75.4% GCMS; 39.5%, 80.8% GCMSMS) was the major prominent compound identified in the C. reticulata peel essential oils in fresh and dried peels respectively, followed by linalool (12.8%, 2.9% GCMS; 48.4%, 7.4% GCMSMS). Percentage yields for the essential oils from C. clementine fresh and dried leaves and peels ranges from 0.40-0.62% v/w for. Fresh and dried leaves volatile oils had sabinene (22.7-27.1%) for GCMS and GCMSMS respectively), linalool (18.5- 20.9% GCMS and GCMSMS) and terpinene-4-ol (6.5- 11.8% GCMS and GCMSMS) as main compounds while fresh and dried peels oils were dominated by limonene (38.9- 86.5% GCMS and GCMSMS). Phytochemical screening experiment of the three citrus species leaves and peels aqueous extract reveal the prominent presence of flavonoids and phenolic compounds in high concentrations. C. reticulata and C. clementine peels and leaves volatile oils exhibited higher antioxidant activity as DPPH free radical scavengers and Ferric reducing power (FRAP) ability, when compared to C. sinensis essential oils which exhibited minimum antioxidant activity. Antioxidant activity of leaves and peels essential oils maybe due to the presence of oxygenated monoterpenes and high limonene content. C. sinensis peels oils showed non-toxic to extremely very low toxicity effect at highest dose 2000 mg/kg. C. reticulata and C. clementine peels oils showed toxicity effect in mice at high dose levels. It is suggested that citrus volatile oils could be safe to use at lower dose levels. Citrus species peels and leaves volatile compounds limonene linalool and sabinene exhibited antiinflammatory activity by significantly reducing rats paw oedema size induced by fresh egg albumin. The study suggests the use of Citrus waste products (leaves and peels) as natural antioxidants with antiinflammatory properties for medicinal value and volatile oils compounds for aroma in beverages and cosmetic industries.
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Prevalence and molecular analysis of multidrug-resistant Acinetobacter baumannii in the extra-hospital environment in Mthatha, South Africa
- Adjei, Anane Yaw, Apalata, Teke, Vasaikar, Sandeep, Okuthe, Grace Emily, Songca, Sandile
- Authors: Adjei, Anane Yaw , Apalata, Teke , Vasaikar, Sandeep , Okuthe, Grace Emily , Songca, Sandile
- Date: 2019
- Subjects: Acinetobacter baumannii , Carbapenemase-encoding genes , Multidrug-resistance , ISAba1 , intI1
- Language: English
- Type: article , text
- Identifier: http://hdl.handle.net/11260/2409 , vital:41457 , https://doi.org/10.1016/j.bjid.2019.09.004
- Description: The presence of Acinetobacter baumannii outside hospitals remains unclear. This study aimed to determine the prevalence of multidrug-resistance (MDR) A. baumannii in the extra-hospital environment in Mthatha, South Africa and to investigate the frequency of carbapenemase-encoding genes. Material and Methods: From August 2016 to July 2017 a total of 598 abattoir samples and 689 aquatic samples were collected and analyzed presumptively by cultural methods for the presence of A. baumannii using CHROMagarTM Acinetobacter medium. Species identification was performed by autoSCAN-4 (Dade Behring Inc., IL) and confirmed by the detection of their intrinsic blaOXA-51 gene. Confirmed MDR A. baumannii isolates were screened for the presence of carbapenemase-encoding genes, ISAba1 insertion sequence and integrase intI1. Results: In total, 248 (19.3%) Acinetobacter species were isolated. Acinetobacter. baumannii was detected in 183 (73.8%) of which 85 (46.4%) and 98 (53.6%) were recovered from abattoir and aquatic respectively. MDR A. baumannii was detected in 56.5% (48/85) abattoir isolates and 53.1% (52/98) aquatic isolates. Isolates showed high resistance to antimicrobials most frequently used to treat Acinetobacter infections such as piperacillin/tazobactam; abattoir (98% of isolates resistant), aquatic (94% of isolates resistant), ceftazidime (84%, 83%), ciprofloxacin (71%, 70%), amikacin (41%, 42%), imipenem (75%, 73%), and meropenem (74%, 71%). All the isolates were susceptible to tigecycline and colistin. All the isolates carried blaOXA-51-like. The blaOXA-23 was detected in 32 (66.7%) abattoir isolates and 11 (21.2%) aquatic isolates. The blaOXA-58-like was positive in 7 (14.6%) and 4 (7.7%) abattoir and aquatic isolates, respectively. Both groups of isolates lacked blaOXA-24-like, blaIMP-type, blaVIM-type, blaNDM-1, blaSIM, blaAmpC, ISAba1 and inI1. Isolates showed high level of Multiple Antibiotic Resistance Index (MARI) ranging from 0.20-0.52. Conclusion: Extra-hospital sources such as abattoir and aquatic environments may be a vehicle of spread of MDR A. baumannii strains in the community and hospital settings.
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- Authors: Adjei, Anane Yaw , Apalata, Teke , Vasaikar, Sandeep , Okuthe, Grace Emily , Songca, Sandile
- Date: 2019
- Subjects: Acinetobacter baumannii , Carbapenemase-encoding genes , Multidrug-resistance , ISAba1 , intI1
- Language: English
- Type: article , text
- Identifier: http://hdl.handle.net/11260/2409 , vital:41457 , https://doi.org/10.1016/j.bjid.2019.09.004
- Description: The presence of Acinetobacter baumannii outside hospitals remains unclear. This study aimed to determine the prevalence of multidrug-resistance (MDR) A. baumannii in the extra-hospital environment in Mthatha, South Africa and to investigate the frequency of carbapenemase-encoding genes. Material and Methods: From August 2016 to July 2017 a total of 598 abattoir samples and 689 aquatic samples were collected and analyzed presumptively by cultural methods for the presence of A. baumannii using CHROMagarTM Acinetobacter medium. Species identification was performed by autoSCAN-4 (Dade Behring Inc., IL) and confirmed by the detection of their intrinsic blaOXA-51 gene. Confirmed MDR A. baumannii isolates were screened for the presence of carbapenemase-encoding genes, ISAba1 insertion sequence and integrase intI1. Results: In total, 248 (19.3%) Acinetobacter species were isolated. Acinetobacter. baumannii was detected in 183 (73.8%) of which 85 (46.4%) and 98 (53.6%) were recovered from abattoir and aquatic respectively. MDR A. baumannii was detected in 56.5% (48/85) abattoir isolates and 53.1% (52/98) aquatic isolates. Isolates showed high resistance to antimicrobials most frequently used to treat Acinetobacter infections such as piperacillin/tazobactam; abattoir (98% of isolates resistant), aquatic (94% of isolates resistant), ceftazidime (84%, 83%), ciprofloxacin (71%, 70%), amikacin (41%, 42%), imipenem (75%, 73%), and meropenem (74%, 71%). All the isolates were susceptible to tigecycline and colistin. All the isolates carried blaOXA-51-like. The blaOXA-23 was detected in 32 (66.7%) abattoir isolates and 11 (21.2%) aquatic isolates. The blaOXA-58-like was positive in 7 (14.6%) and 4 (7.7%) abattoir and aquatic isolates, respectively. Both groups of isolates lacked blaOXA-24-like, blaIMP-type, blaVIM-type, blaNDM-1, blaSIM, blaAmpC, ISAba1 and inI1. Isolates showed high level of Multiple Antibiotic Resistance Index (MARI) ranging from 0.20-0.52. Conclusion: Extra-hospital sources such as abattoir and aquatic environments may be a vehicle of spread of MDR A. baumannii strains in the community and hospital settings.
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The prevalence of insufficient iodine intake in pregnancy in Africa: protocol for a systematic review and meta-analysis
- Businge, Charles Bitamazire, Longo-Mbenza , Benjamin, Kengne, Andre Pascal
- Authors: Businge, Charles Bitamazire , Longo-Mbenza , Benjamin , Kengne, Andre Pascal
- Date: 2019
- Subjects: Africa , Iodine , Pregnancy
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/5882 , vital:44662 , https://DOI:10.1186/s13643-019-1092-7
- Description: Background: Insufficient iodine intake in pregnancy is associated with many adverse pregnancy outcomes. About 90% of African countries are at risk of iodine deficiency due to poor soils and dietary goitrogens. Pregnancy predisposes to insufficient iodine nutrition secondary to increased physiological demand and increased renal loss. Iodine deficiency is re-emerging in countries thought to be replete with pregnant women being the most affected. This review seeks to identify the degree of iodine nutrition in pregnancy on the entire African continent before and after the implementation of national iodization programmes. Methods: A systematic search of published literature will be conducted for observational studies that directly determined the prevalence of insufficient iodine intake among pregnant women in Africa. Electronic databases and grey literature will be searched for baseline data before the implementation of population-based iodine supplementation and for follow-up data up to December 2018. Screening of identified articles and data extraction will be conducted independently by two investigators. Risk of bias and methodological quality of the included studies will be assessed using a risk of bias tool. Appropriate meta-analytic techniques will be used to pool prevalence estimates from studies with similar features, overall and by major characteristics including the region of the study, time period (before and after implementation of iodization programmes), sample size and age. Heterogeneity of the estimates across studies will be quantified and publication bias investigated. This protocol is reported according to Preferred Reporting Items for Systematic reviews and Meta-Analysis protocols (PRISMA-P) 2015 guidelines. Discussion: This review will help ascertain the impact of national iodization programmes on the iodine nutrition status in pregnancy in Africa and advise policy on the necessity for monitoring and mitigating iodine deficiency in pregnancy in Africa. This review is part of a thesis that will be submitted to the Faculty of Health Sciences, University of Cape Town, for the award of a PhD in Medicine whose protocol has been granted ethics approval (UCT HREC 135/2018). In addition, the results will be published in a peer-reviewed journal. Systematic review registration: PROSPERO CRD42018099434 Keywords: Iodine, Insufficiency, Pregnancy, Africa
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- Authors: Businge, Charles Bitamazire , Longo-Mbenza , Benjamin , Kengne, Andre Pascal
- Date: 2019
- Subjects: Africa , Iodine , Pregnancy
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/5882 , vital:44662 , https://DOI:10.1186/s13643-019-1092-7
- Description: Background: Insufficient iodine intake in pregnancy is associated with many adverse pregnancy outcomes. About 90% of African countries are at risk of iodine deficiency due to poor soils and dietary goitrogens. Pregnancy predisposes to insufficient iodine nutrition secondary to increased physiological demand and increased renal loss. Iodine deficiency is re-emerging in countries thought to be replete with pregnant women being the most affected. This review seeks to identify the degree of iodine nutrition in pregnancy on the entire African continent before and after the implementation of national iodization programmes. Methods: A systematic search of published literature will be conducted for observational studies that directly determined the prevalence of insufficient iodine intake among pregnant women in Africa. Electronic databases and grey literature will be searched for baseline data before the implementation of population-based iodine supplementation and for follow-up data up to December 2018. Screening of identified articles and data extraction will be conducted independently by two investigators. Risk of bias and methodological quality of the included studies will be assessed using a risk of bias tool. Appropriate meta-analytic techniques will be used to pool prevalence estimates from studies with similar features, overall and by major characteristics including the region of the study, time period (before and after implementation of iodization programmes), sample size and age. Heterogeneity of the estimates across studies will be quantified and publication bias investigated. This protocol is reported according to Preferred Reporting Items for Systematic reviews and Meta-Analysis protocols (PRISMA-P) 2015 guidelines. Discussion: This review will help ascertain the impact of national iodization programmes on the iodine nutrition status in pregnancy in Africa and advise policy on the necessity for monitoring and mitigating iodine deficiency in pregnancy in Africa. This review is part of a thesis that will be submitted to the Faculty of Health Sciences, University of Cape Town, for the award of a PhD in Medicine whose protocol has been granted ethics approval (UCT HREC 135/2018). In addition, the results will be published in a peer-reviewed journal. Systematic review registration: PROSPERO CRD42018099434 Keywords: Iodine, Insufficiency, Pregnancy, Africa
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Ursolic Acid and its derivatives as bioactive agents
- Mlala, Sithenkosi, Oyedeji, Adebola Omowunmi, Gondwe, Mavuto, Oyedeji, Opeoluwa Oyehan
- Authors: Mlala, Sithenkosi , Oyedeji, Adebola Omowunmi , Gondwe, Mavuto , Oyedeji, Opeoluwa Oyehan
- Date: 2019
- Subjects: Noncommunicable diseases , Pentacyclic triterpenoids , Ursolic acid , Clinical trials
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/1576 , vital:37793 , https://doi.org/10.3390/molecules24152751
- Description: Non-communicable diseases (NCDs) such as cancer, diabetes, and chronic respiratory and cardiovascular diseases continue to be threatening and deadly to human kind. Resistance to and side effects of known drugs for treatment further increase the threat, while at the same time leaving scientists to search for alternative sources from nature, especially from plants. Pentacyclic triterpenoids (PT) from medicinal plants have been identified as one class of secondary metabolites that could play a critical role in the treatment and management of several NCDs. One of such PT is ursolic acid (UA, 3 β-hydroxy-urs-12-en-28-oic acid), which possesses important biological effects, including anti-inflammatory, anticancer, antidiabetic, antioxidant and antibacterial effects, but its bioavailability and solubility limits its clinical application. Mimusops caffra, Ilex paraguarieni, and Glechoma hederacea, have been reported as major sources of UA. The chemistry of UA has been studied extensively based on the literature, with modifications mostly having been made at positions C-3 (hydroxyl), C12-C13 (double bonds) and C-28 (carboxylic acid), leading to several UA derivatives (esters, amides, oxadiazole quinolone, etc.) with enhanced potency, bioavailability and water solubility. This article comprehensively reviews the information that has become available over the last decade with respect to the sources, chemistry, biological potency and clinical trials of UA and its derivatives as potential therapeutic agents, with a focus on addressing NCD.
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- Authors: Mlala, Sithenkosi , Oyedeji, Adebola Omowunmi , Gondwe, Mavuto , Oyedeji, Opeoluwa Oyehan
- Date: 2019
- Subjects: Noncommunicable diseases , Pentacyclic triterpenoids , Ursolic acid , Clinical trials
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/1576 , vital:37793 , https://doi.org/10.3390/molecules24152751
- Description: Non-communicable diseases (NCDs) such as cancer, diabetes, and chronic respiratory and cardiovascular diseases continue to be threatening and deadly to human kind. Resistance to and side effects of known drugs for treatment further increase the threat, while at the same time leaving scientists to search for alternative sources from nature, especially from plants. Pentacyclic triterpenoids (PT) from medicinal plants have been identified as one class of secondary metabolites that could play a critical role in the treatment and management of several NCDs. One of such PT is ursolic acid (UA, 3 β-hydroxy-urs-12-en-28-oic acid), which possesses important biological effects, including anti-inflammatory, anticancer, antidiabetic, antioxidant and antibacterial effects, but its bioavailability and solubility limits its clinical application. Mimusops caffra, Ilex paraguarieni, and Glechoma hederacea, have been reported as major sources of UA. The chemistry of UA has been studied extensively based on the literature, with modifications mostly having been made at positions C-3 (hydroxyl), C12-C13 (double bonds) and C-28 (carboxylic acid), leading to several UA derivatives (esters, amides, oxadiazole quinolone, etc.) with enhanced potency, bioavailability and water solubility. This article comprehensively reviews the information that has become available over the last decade with respect to the sources, chemistry, biological potency and clinical trials of UA and its derivatives as potential therapeutic agents, with a focus on addressing NCD.
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