Molecular Detection of Antibiotic-Resistant Genes in Pseudomonas aeruginosa from Nonclinical Environment: Public Health Implications in Mthatha, Eastern Cape Province, South Africa
- Mojisola Clara Hosu, Sandeep Vasaikar, Grace Emily Okuthe, Teke Apalata
- Authors: Mojisola Clara Hosu , Sandeep Vasaikar , Grace Emily Okuthe , Teke Apalata
- Date: 5 January 2021
- Subjects: Microbiology
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/2417 , vital:41877
- Description: Evaluation of resistant profiles and detection of antimicrobial-resistant genes of bacterial pathogens in the nonclinical milieu is imperative to assess the probable risk of dissemination of resistant genes in the environment. .is paper sought to identify antibiotic-resistant genes in Pseudomonas aeruginosa from nonclinical sources in Mthatha, Eastern Cape, and evaluate its public health implications. Samples collected from abattoir wastewater and aquatic environment were processed by membrane filtration and cultured on CHROMagarTM Pseudomonas medium. Species identification was performed by autoSCAN-4 (Dade Behring Inc., IL). Molecular characterization of the isolates was confirmed using real-time polymerase chain reaction (rPCR) and selected isolates were further screened for the possibility of harboring antimicrobial resistance genes. Fifty-one Pseudomonas species were recovered from abattoir wastewater and surface water samples, out of which thirty-six strains were Pseudomonas aeruginosa (70.6%). .e P. aeruginosa isolates demonstrated resistance to aztreonam (86.1%), ceftazidime (63.9%), piperacillin (58.3%), cefepime (55.6%), imipenem (50%), piperacillin/tazobactam (47.2%), meropenem (41.7%), and levofloxacin (30.6%). Twenty out of thirty-six P. aeruginosa displayed multidrug resistance profiles and were classified as multidrug-resistant (MDR) (55.6%). Most of the bacterial isolates exhibited a high Multiple Antibiotic Resistance (MAR) Index ranging from 0.08 to 0.69 with a mean MAR index of 0.38. In the rPCR analysis of fifteen P. aeruginosa isolates, 14 isolates (93.3%) were detected harboring blaSHV, six isolates (40%) harbored blaTEM, and three isolates (20%) harbored blaCTX-M, being the least occurring ESBL. Results of the current study revealed that P. aeruginosa isolates recovered from nonclinical milieu are resistant to frontline clinically relevant antipseudomonal drugs. .is is concerning as it poses a risk to the environment and constitutes a public health threat. Given the public health relevance, the paper recommends monitoring of multidrug-resistant pathogens in effluent environments.
- Full Text:
- Authors: Mojisola Clara Hosu , Sandeep Vasaikar , Grace Emily Okuthe , Teke Apalata
- Date: 5 January 2021
- Subjects: Microbiology
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/2417 , vital:41877
- Description: Evaluation of resistant profiles and detection of antimicrobial-resistant genes of bacterial pathogens in the nonclinical milieu is imperative to assess the probable risk of dissemination of resistant genes in the environment. .is paper sought to identify antibiotic-resistant genes in Pseudomonas aeruginosa from nonclinical sources in Mthatha, Eastern Cape, and evaluate its public health implications. Samples collected from abattoir wastewater and aquatic environment were processed by membrane filtration and cultured on CHROMagarTM Pseudomonas medium. Species identification was performed by autoSCAN-4 (Dade Behring Inc., IL). Molecular characterization of the isolates was confirmed using real-time polymerase chain reaction (rPCR) and selected isolates were further screened for the possibility of harboring antimicrobial resistance genes. Fifty-one Pseudomonas species were recovered from abattoir wastewater and surface water samples, out of which thirty-six strains were Pseudomonas aeruginosa (70.6%). .e P. aeruginosa isolates demonstrated resistance to aztreonam (86.1%), ceftazidime (63.9%), piperacillin (58.3%), cefepime (55.6%), imipenem (50%), piperacillin/tazobactam (47.2%), meropenem (41.7%), and levofloxacin (30.6%). Twenty out of thirty-six P. aeruginosa displayed multidrug resistance profiles and were classified as multidrug-resistant (MDR) (55.6%). Most of the bacterial isolates exhibited a high Multiple Antibiotic Resistance (MAR) Index ranging from 0.08 to 0.69 with a mean MAR index of 0.38. In the rPCR analysis of fifteen P. aeruginosa isolates, 14 isolates (93.3%) were detected harboring blaSHV, six isolates (40%) harbored blaTEM, and three isolates (20%) harbored blaCTX-M, being the least occurring ESBL. Results of the current study revealed that P. aeruginosa isolates recovered from nonclinical milieu are resistant to frontline clinically relevant antipseudomonal drugs. .is is concerning as it poses a risk to the environment and constitutes a public health threat. Given the public health relevance, the paper recommends monitoring of multidrug-resistant pathogens in effluent environments.
- Full Text:
Morphology, histology and histochemistry of the digestive tract of the Banded tilapia, Tilapia sparrmanii (Perciformes: Cichlidae)
- Grace E. Okuthe, Bongile Bhomela
- Authors: Grace E. Okuthe , Bongile Bhomela
- Date: December 23, 2020
- Subjects: Fish, gastrointestinal tract, histo-architecture
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/2425 , vital:41881
- Description: ABSTRACT. This study described anatomical, histological and histochemical features of the mucosal layer of the digestive tract of Tilapia sparrmanii Smith, 1840, an omnivorous freshwater fish endemic to Southern Africa. This species exhibited a short thick oesophagus with long deep longitudinal folds (466.68 ± 16.91 µm), and a thick (173.50 ± 10.92 µm) muscular layer that allow the passage of large food items. The mucosa was lined with stratified secretory epithelium rich in goblet cells that secreted neutral and acid mucins. The stomach was a sac-like structure with simple tubular glands surrounded by connective tissue. The mucosa was lined with simple columnar epithelium and the lamina propria exhibited a well-developed layer of gastric glands that occupied the entire length of the cardio-fundic region. The stomach mucosa consisted of epithelial cells with intense neutral mucin secretion which protects against gastric juice. Neck cells of gastric glands synthesized neutral and acid mucins. The intestine was highly coiled and presented a complex pattern of transversal folds internally (villi). Villi length decreased progressively from the anterior to the posterior intestine (p < 0.0001). Tunica muscularis of the mid-intestine had the thinnest thickness among all parts of the intestine (p < 0.0001). Goblet cells whose numbers increased towards the rectum secreted both acid and neutral mucins. The results indicate structural similarities of T. sparrmanii GIT with other tilapia species and will be useful for understanding the physiology of the digestive systems as well as functional components of the GIT.
- Full Text:
- Authors: Grace E. Okuthe , Bongile Bhomela
- Date: December 23, 2020
- Subjects: Fish, gastrointestinal tract, histo-architecture
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/2425 , vital:41881
- Description: ABSTRACT. This study described anatomical, histological and histochemical features of the mucosal layer of the digestive tract of Tilapia sparrmanii Smith, 1840, an omnivorous freshwater fish endemic to Southern Africa. This species exhibited a short thick oesophagus with long deep longitudinal folds (466.68 ± 16.91 µm), and a thick (173.50 ± 10.92 µm) muscular layer that allow the passage of large food items. The mucosa was lined with stratified secretory epithelium rich in goblet cells that secreted neutral and acid mucins. The stomach was a sac-like structure with simple tubular glands surrounded by connective tissue. The mucosa was lined with simple columnar epithelium and the lamina propria exhibited a well-developed layer of gastric glands that occupied the entire length of the cardio-fundic region. The stomach mucosa consisted of epithelial cells with intense neutral mucin secretion which protects against gastric juice. Neck cells of gastric glands synthesized neutral and acid mucins. The intestine was highly coiled and presented a complex pattern of transversal folds internally (villi). Villi length decreased progressively from the anterior to the posterior intestine (p < 0.0001). Tunica muscularis of the mid-intestine had the thinnest thickness among all parts of the intestine (p < 0.0001). Goblet cells whose numbers increased towards the rectum secreted both acid and neutral mucins. The results indicate structural similarities of T. sparrmanii GIT with other tilapia species and will be useful for understanding the physiology of the digestive systems as well as functional components of the GIT.
- Full Text:
The prevalence of insufficient iodine intake in pregnancy in Africa: Protocol for a systematic review and meta-analysis
- Charles Bitamazire Businge, Benjamin Longo-Mbenza, Andre Pascal Kengne
- Authors: Charles Bitamazire Businge , Benjamin Longo-Mbenza , Andre Pascal Kengne
- Date: 22-8-2019
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/3387 , vital:43338 , https://systematicreviewsjournal.biomedcentral.com/articles/10.1186/s13643-019-1092-7#citeas
- Description: Background: Insufficient iodine intake in pregnancy is associated with many adverse pregnancy outcomes. About 90% of African countries are at risk of iodine deficiency due to poor soils and dietary goitrogens. Pregnancy predisposes to insufficient iodine nutrition secondary to increased physiological demand and increased renal loss. Iodine deficiency is re-emerging in countries thought to be replete with pregnant women being the most affected. This review seeks to identify the degree of iodine nutrition in pregnancy on the entire African continent before and after the implementation of national iodization programmes. Methods: A systematic search of published literature will be conducted for observational studies that directly determined the prevalence of insufficient iodine intake among pregnant women in Africa. Electronic databases and grey literature will be searched for baseline data before the implementation of population-based iodine supplementation and for follow-up data up to December 2018. Screening of identified articles and data extraction will be conducted independently by two investigators. Risk of bias and methodological quality of the included studies will be assessed using a risk of bias tool. Appropriate meta-analytic techniques will be used to pool prevalence estimates from studies with similar features, overall and by major characteristics including the region of the study, time period (before and after implementation of iodization programmes), sample size and age. Heterogeneity of the estimates across studies will be quantified and publication bias investigated. This protocol is reported according to Preferred Reporting Items for Systematic reviews and Meta-Analysis protocols (PRISMA-P) 2015 guidelines. Discussion This review will help ascertain the impact of national iodization programmes on the iodine nutrition status in pregnancy in Africa and advise policy on the necessity for monitoring and mitigating iodine deficiency in pregnancy in Africa. This review is part of a thesis that will be submitted to the Faculty of Health Sciences, University of Cape Town, for the award of a PhD in Medicine whose protocol has been granted ethics approval (UCT HREC 135/2018). In addition, the results will be published in a peer-reviewed journal.
- Full Text:
- Authors: Charles Bitamazire Businge , Benjamin Longo-Mbenza , Andre Pascal Kengne
- Date: 22-8-2019
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/3387 , vital:43338 , https://systematicreviewsjournal.biomedcentral.com/articles/10.1186/s13643-019-1092-7#citeas
- Description: Background: Insufficient iodine intake in pregnancy is associated with many adverse pregnancy outcomes. About 90% of African countries are at risk of iodine deficiency due to poor soils and dietary goitrogens. Pregnancy predisposes to insufficient iodine nutrition secondary to increased physiological demand and increased renal loss. Iodine deficiency is re-emerging in countries thought to be replete with pregnant women being the most affected. This review seeks to identify the degree of iodine nutrition in pregnancy on the entire African continent before and after the implementation of national iodization programmes. Methods: A systematic search of published literature will be conducted for observational studies that directly determined the prevalence of insufficient iodine intake among pregnant women in Africa. Electronic databases and grey literature will be searched for baseline data before the implementation of population-based iodine supplementation and for follow-up data up to December 2018. Screening of identified articles and data extraction will be conducted independently by two investigators. Risk of bias and methodological quality of the included studies will be assessed using a risk of bias tool. Appropriate meta-analytic techniques will be used to pool prevalence estimates from studies with similar features, overall and by major characteristics including the region of the study, time period (before and after implementation of iodization programmes), sample size and age. Heterogeneity of the estimates across studies will be quantified and publication bias investigated. This protocol is reported according to Preferred Reporting Items for Systematic reviews and Meta-Analysis protocols (PRISMA-P) 2015 guidelines. Discussion This review will help ascertain the impact of national iodization programmes on the iodine nutrition status in pregnancy in Africa and advise policy on the necessity for monitoring and mitigating iodine deficiency in pregnancy in Africa. This review is part of a thesis that will be submitted to the Faculty of Health Sciences, University of Cape Town, for the award of a PhD in Medicine whose protocol has been granted ethics approval (UCT HREC 135/2018). In addition, the results will be published in a peer-reviewed journal.
- Full Text:
Determination of Carbapenemases and Colistin Resistance in the Clinical Isolates from Healthcare Facilities in Mthatha and Surrounding Areas
- Authors: Ndlela, Silindokuhle
- Date: 2025
- Subjects: Antimicrobial resistance , Healthcare facilities , Clinical Isolates , Mthatha
- Language: English
- Type: Masters (Thesis)
- Identifier: http://hdl.handle.net/11260/13473 , vital:78935
- Description: Antimicrobial resistance is associated with the emergence of 3 carbapenemase enzymes that hydrolyze carbapenem antibiotics. These enzymes 4 are carried by certain carbapenemase-producing genes. The emergence of 5 carbapenem resistant Enterobacterales (CRE) has become a serious public health 6 threat in South Africa and globally, and has led to the use of colistin for treating 7 severe infections. The aim of the study is to determine the prevalence of 8 carbapenemase-producing genes, the minimum inhibitory concentration (MIC) for 9 the antibiotic colistin, and the prevalence of the mobile colistin resistant gene mcr 10 1 in clinical isolates drawn from healthcare facilities in Mthatha and surrounding 11 areas… , Degree: M. Science (Medical Microbiology)
- Full Text:
- Authors: Ndlela, Silindokuhle
- Date: 2025
- Subjects: Antimicrobial resistance , Healthcare facilities , Clinical Isolates , Mthatha
- Language: English
- Type: Masters (Thesis)
- Identifier: http://hdl.handle.net/11260/13473 , vital:78935
- Description: Antimicrobial resistance is associated with the emergence of 3 carbapenemase enzymes that hydrolyze carbapenem antibiotics. These enzymes 4 are carried by certain carbapenemase-producing genes. The emergence of 5 carbapenem resistant Enterobacterales (CRE) has become a serious public health 6 threat in South Africa and globally, and has led to the use of colistin for treating 7 severe infections. The aim of the study is to determine the prevalence of 8 carbapenemase-producing genes, the minimum inhibitory concentration (MIC) for 9 the antibiotic colistin, and the prevalence of the mobile colistin resistant gene mcr 10 1 in clinical isolates drawn from healthcare facilities in Mthatha and surrounding 11 areas… , Degree: M. Science (Medical Microbiology)
- Full Text:
Reimagining library services: Transforming libraries into dynamic learning spaces in South African universities
- Authors: Nongalo, Noloyiso
- Date: 2025
- Subjects: Dynamic learning spaces , Collaborative enviroments , Inclusive education , Digital Literacy , Lifelong learning
- Language: English
- Type: article
- Identifier: http://hdl.handle.net/11260/13140 , vital:77950 , DOI: https://doi.org/10.38159/ehass.2025641
- Description: This conceptual paper aimed to explore the transformation of university libraries in South Africa into dynamic learning spaces that cater to evolving academic and social needs of students. In the context of South Africa's rapidly changing higher education landscape, where inclusivity and technological advancements are paramount, the role of university libraries is shifting from traditional information repositories to hubs of collaborative learning and student engagement. The paper employed a qualitative review of literature and an analysis of the existing infrastructures in libraries of Historically Disadvantaged Institutions (HDIs) to identify strategies for this transformation. The findings highlighted the pivotal role libraries play in promoting lifelong learning, enhancing digital literacy, and supporting academic success. Key recommendations include the integration of advanced technology, the creation of flexible, student-centred spaces, and the incorporation of inclusive practices to accommodate diverse student populations. This paper contributes to ongoing research by offering a model for reimagining library spaces in line with global trends, providing insights for other educational institutions seeking to foster dynamic, student-focused learning environments.
- Full Text:
- Authors: Nongalo, Noloyiso
- Date: 2025
- Subjects: Dynamic learning spaces , Collaborative enviroments , Inclusive education , Digital Literacy , Lifelong learning
- Language: English
- Type: article
- Identifier: http://hdl.handle.net/11260/13140 , vital:77950 , DOI: https://doi.org/10.38159/ehass.2025641
- Description: This conceptual paper aimed to explore the transformation of university libraries in South Africa into dynamic learning spaces that cater to evolving academic and social needs of students. In the context of South Africa's rapidly changing higher education landscape, where inclusivity and technological advancements are paramount, the role of university libraries is shifting from traditional information repositories to hubs of collaborative learning and student engagement. The paper employed a qualitative review of literature and an analysis of the existing infrastructures in libraries of Historically Disadvantaged Institutions (HDIs) to identify strategies for this transformation. The findings highlighted the pivotal role libraries play in promoting lifelong learning, enhancing digital literacy, and supporting academic success. Key recommendations include the integration of advanced technology, the creation of flexible, student-centred spaces, and the incorporation of inclusive practices to accommodate diverse student populations. This paper contributes to ongoing research by offering a model for reimagining library spaces in line with global trends, providing insights for other educational institutions seeking to foster dynamic, student-focused learning environments.
- Full Text:
Integration of HIV/ AIDS, Tuberculosis and Patients’ Services into the General Health Care Systems: Implementation Challenges and Impact on TB Control Programme in O.R. Tambo District, Eastern Cape, South Africa.
- Authors: Dlatu, Ntandazo
- Date: 2024-08
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/11260/12987 , vital:76186
- Description: Tuberculosis (TB), a disease of poverty and inequality, is a leading cause of severe illness and death among people with human immunodeficiency virus (HIV). In South Africa, both TB and HIV epidemics have been closely related and persistent, posing a significant burden for healthcare provision. Reducing morbidity and mortality from HIV-associated TB requires strong collaboration between TB and HIV services at all levels with fully integrated, people-centered models of care. Studies have observed that TB and HIV integration reduces mortality. However, the operational implementation of integrated services is still challenging. Gaps exist between the challenges of combining TB and HIV control with evidence-based knowledge, affecting the implementation process of this highly recommended strategy. Despite the policy, frameworks for integration exist; integration of TB and HIV services is far from ideal in many resource-limited countries, including South Africa. Few studies have examined the advantages and disadvantages of integrated TB and HIV care in public health facilities, and even fewer have proposed conceptual models for proven integration. Objectives: The goals of this study consisted of: (i) investigating the associations between socio-economic deprivation and tuberculosis (TB) treatment outcomes, alongside well-known TB risk factors. The effects of healthcare expenditures and their growth on trends in TB incidence from 2009 to 2013 were also assessed. (ii) Exploring patients’ and healthcare workers’ perceptions of TB and HIV integration as well as a strategy for integrating these programs. (iii) Describing patients’ perceptions on barriers to scaling up of TB-HIV integration services at selected health facilities; and (iv) Developing a paradigm for integrating TB, HIV, and patient services in a single facility and highlighting the importance of TB-HIV services for greater accessibility under one roof. Methods: In Phase 1 of the study, secondary data analysis was performed on data obtained from various sources including governmental, non-governmental, and research institutions. Indicators for TB treatment outcomes included TB death rate, TB rate among the household contacts of the Index TB cases, TB treatment failure, HIV-associated TB death rate, TB defaulter rate, and new TB smear-positive cases. Analysis of variance (ANOVA) and Turkey’s tests for post-hoc analysis were used to P a g e xvii | compare means of variables of interest considering a type I error rate of 0.05. , Thesis (PhD) -- Faculty of Medicine and Health Sciences, 2024
- Full Text:
- Authors: Dlatu, Ntandazo
- Date: 2024-08
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/11260/12987 , vital:76186
- Description: Tuberculosis (TB), a disease of poverty and inequality, is a leading cause of severe illness and death among people with human immunodeficiency virus (HIV). In South Africa, both TB and HIV epidemics have been closely related and persistent, posing a significant burden for healthcare provision. Reducing morbidity and mortality from HIV-associated TB requires strong collaboration between TB and HIV services at all levels with fully integrated, people-centered models of care. Studies have observed that TB and HIV integration reduces mortality. However, the operational implementation of integrated services is still challenging. Gaps exist between the challenges of combining TB and HIV control with evidence-based knowledge, affecting the implementation process of this highly recommended strategy. Despite the policy, frameworks for integration exist; integration of TB and HIV services is far from ideal in many resource-limited countries, including South Africa. Few studies have examined the advantages and disadvantages of integrated TB and HIV care in public health facilities, and even fewer have proposed conceptual models for proven integration. Objectives: The goals of this study consisted of: (i) investigating the associations between socio-economic deprivation and tuberculosis (TB) treatment outcomes, alongside well-known TB risk factors. The effects of healthcare expenditures and their growth on trends in TB incidence from 2009 to 2013 were also assessed. (ii) Exploring patients’ and healthcare workers’ perceptions of TB and HIV integration as well as a strategy for integrating these programs. (iii) Describing patients’ perceptions on barriers to scaling up of TB-HIV integration services at selected health facilities; and (iv) Developing a paradigm for integrating TB, HIV, and patient services in a single facility and highlighting the importance of TB-HIV services for greater accessibility under one roof. Methods: In Phase 1 of the study, secondary data analysis was performed on data obtained from various sources including governmental, non-governmental, and research institutions. Indicators for TB treatment outcomes included TB death rate, TB rate among the household contacts of the Index TB cases, TB treatment failure, HIV-associated TB death rate, TB defaulter rate, and new TB smear-positive cases. Analysis of variance (ANOVA) and Turkey’s tests for post-hoc analysis were used to P a g e xvii | compare means of variables of interest considering a type I error rate of 0.05. , Thesis (PhD) -- Faculty of Medicine and Health Sciences, 2024
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Exploring the experience of clients on fixed-dose combination antiretroviral drugs at Ngangelizwe Community Health Centre, Eastern Cape
- Authors: Macozoma, Wandile
- Date: 2024-07
- Language: English
- Type: Masters , text
- Identifier: http://hdl.handle.net/11260/12963 , vital:76182
- Description: Fixed dose combination antiretroviral drugs are multiple retroviral drugs combined into a single pill, commenced in South Africa on 01 April 2013 after it was announced by the Minister of Health. The main reason for this change of treatment was to reduce pill burden and make the lives of people living with HIV and who are on ARVs simpler and easier. The purpose of this study was to describe experiences of clients on fixed dose combination antiretroviral drug at Ngangelizwe Community Health Centre, Eastern Cape. The study used a qualitative research approach and descriptive phenomenology as a research design. The target population for this study were clients initiated on a fixed dose combination antiretroviral drug. Purposive sampling was done and semi-structured individual interviews conducted to collect data, audiotape was used and field notes taken. Data saturation was reached after interviewing 12 participants. Data analysis was done guided by Tesch’s 8 steps of the coding process. Ethical principles were adhered to and informed consent obtained from participants. Findings revealed that there were physical, psychological and social experiences, from the period of first diagnosis, disclosure and medication compliance. It is recommended that health education campaigns be intensified since there is still stigmatization of HIV/AIDS that affects compliance. Also, there is need for continuing education and training for healthcare providers to encourage a supportive attitude towards clients. , Thesis (Masters) -- Faculty of Medicine and Health Sciences, 2024
- Full Text:
- Authors: Macozoma, Wandile
- Date: 2024-07
- Language: English
- Type: Masters , text
- Identifier: http://hdl.handle.net/11260/12963 , vital:76182
- Description: Fixed dose combination antiretroviral drugs are multiple retroviral drugs combined into a single pill, commenced in South Africa on 01 April 2013 after it was announced by the Minister of Health. The main reason for this change of treatment was to reduce pill burden and make the lives of people living with HIV and who are on ARVs simpler and easier. The purpose of this study was to describe experiences of clients on fixed dose combination antiretroviral drug at Ngangelizwe Community Health Centre, Eastern Cape. The study used a qualitative research approach and descriptive phenomenology as a research design. The target population for this study were clients initiated on a fixed dose combination antiretroviral drug. Purposive sampling was done and semi-structured individual interviews conducted to collect data, audiotape was used and field notes taken. Data saturation was reached after interviewing 12 participants. Data analysis was done guided by Tesch’s 8 steps of the coding process. Ethical principles were adhered to and informed consent obtained from participants. Findings revealed that there were physical, psychological and social experiences, from the period of first diagnosis, disclosure and medication compliance. It is recommended that health education campaigns be intensified since there is still stigmatization of HIV/AIDS that affects compliance. Also, there is need for continuing education and training for healthcare providers to encourage a supportive attitude towards clients. , Thesis (Masters) -- Faculty of Medicine and Health Sciences, 2024
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Translanguaging pedagogies and practice for social justice in a multilingual South African previously disadvantaged
- Authors: Mpofu, Tarisai
- Date: 2024-06
- Subjects: Multilingualism
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/11260/11131 , vital:75921
- Description: Over the last two decades, there has been a burgeoning interest in translanguaging as both a theory and a methodology, but a few studies have looked at it as a pedagogy. This has meant that while there is near-consensus on translanguaging’s contribution to knowing, there have been few studies on how it can be implemented systematically in classrooms. This study examines how translanguaging is being practiced at one South African university and makes recommendations on how translanguaging can be implemented systematically for better impact. A mixed method approach was used, meaning that it had both qualitative and quantitative elements. Data for this study were gathered using online questionnaires completed by students and lecturers, interviews with lecturers, and observation. The results show that limited translanguaging was taking place in the classroom, despite both students and staff acknowledging its value to epistemic access. This study calls for intentional and systematic implementation of translanguaging to contribute to socially-just classroom practices. The study proposes the translanguaging matrix model for practice to assist in institutionalising translanguaging through the provision of short learning courses for staff and students on translanguaging, language learning opportunities and clear pedagogical training on when translanguaging must be used in classroom contexts. Key Words: Translanguaging, Language Policy, Social Justice, Multilingalism, Pedagogy , Thesis (D.Ed) -- Faculty of Education, 2024
- Full Text:
- Authors: Mpofu, Tarisai
- Date: 2024-06
- Subjects: Multilingualism
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/11260/11131 , vital:75921
- Description: Over the last two decades, there has been a burgeoning interest in translanguaging as both a theory and a methodology, but a few studies have looked at it as a pedagogy. This has meant that while there is near-consensus on translanguaging’s contribution to knowing, there have been few studies on how it can be implemented systematically in classrooms. This study examines how translanguaging is being practiced at one South African university and makes recommendations on how translanguaging can be implemented systematically for better impact. A mixed method approach was used, meaning that it had both qualitative and quantitative elements. Data for this study were gathered using online questionnaires completed by students and lecturers, interviews with lecturers, and observation. The results show that limited translanguaging was taking place in the classroom, despite both students and staff acknowledging its value to epistemic access. This study calls for intentional and systematic implementation of translanguaging to contribute to socially-just classroom practices. The study proposes the translanguaging matrix model for practice to assist in institutionalising translanguaging through the provision of short learning courses for staff and students on translanguaging, language learning opportunities and clear pedagogical training on when translanguaging must be used in classroom contexts. Key Words: Translanguaging, Language Policy, Social Justice, Multilingalism, Pedagogy , Thesis (D.Ed) -- Faculty of Education, 2024
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Green market emergence as a predictor of green entrepreneurship and sustainable development of small and medium sized manufacturing entities in the North-West province, South Africa
- Authors: Nokemane, Lihle
- Date: 2024-04
- Subjects: Green Market Emergence , Education institutions , South Africa
- Language: English
- Type: Master's/Doctoral theses
- Identifier: http://hdl.handle.net/11260/13095 , vital:77656
- Description: Thesis (MA) -- Faculty of Economics and Financial Sciences, Economics and Business Sciences 2024
- Full Text:
- Authors: Nokemane, Lihle
- Date: 2024-04
- Subjects: Green Market Emergence , Education institutions , South Africa
- Language: English
- Type: Master's/Doctoral theses
- Identifier: http://hdl.handle.net/11260/13095 , vital:77656
- Description: Thesis (MA) -- Faculty of Economics and Financial Sciences, Economics and Business Sciences 2024
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The moderating role of green consumer values in the relationship between green marketing and consumer behaviour:
- Ntshangase, Siyasanga Vuyiswa
- Authors: Ntshangase, Siyasanga Vuyiswa
- Date: 2024-04
- Subjects: Green Consumer Values , Education institutions , South Africa
- Language: English
- Type: Masters
- Identifier: http://hdl.handle.net/11260/13105 , vital:77657
- Description: The study aimed to investigate the moderating role of green consumer values in the relationship between green marketing and consumer behaviour. The study focused on the important role of green consumer values in shaping environmentally conscious consumer actions, emphasizing the importance of considering these values in green marketing strategies.To achieve the aim, the study adopted a positivist paradigm, quantitative approach, and a causal research design. A survey technique was employed to gather primary data where a self-administered questionnaire was issued to 450 respondents who were purposefully sampled. The study’s sample constituted consumers that frequently visit hotels in the Eastern Cape. The key findings of the study suggest that there is a significant role that green marketing plays in consumer behaviour towards green products offered in hotels. Furthermore, the findings highlight how green consumer values influence consumers towards value-driven choices for sustainable practices in the hotel industry, high level of satisfaction and preference for green products. Based on the findings, government and the hospitality industry should implement policies that will provide incentives for hotels that practise sustainable measures. This could include free quarterly advertisement of green hotels, free hampers for consumers that make use of green hotels on a regularly basis, and a spa voucher for every new consumer. Government should also provide capital and resources to small businesses to upscale their establishments to meet green hotel standards, for instance provide funding for solar installation and for those who already have installed solar panels to be reimbursed a fraction of the amount they paid.This study makes a significant contribution by highlighting the important role of green consumer values as a moderator. It reveals how these values impact the relationship between green marketing strategies and consumer behaviour. The study provides valuable insights for marketers, emphasizing the need to align strategies with green consumer values to promote more effective and impactful responses from consumers towards environmentally friendly initiatives. , Thesis (MA) -- Faculty of Economic and Financial Sciences, Business Management and Economics, 2024
- Full Text:
- Authors: Ntshangase, Siyasanga Vuyiswa
- Date: 2024-04
- Subjects: Green Consumer Values , Education institutions , South Africa
- Language: English
- Type: Masters
- Identifier: http://hdl.handle.net/11260/13105 , vital:77657
- Description: The study aimed to investigate the moderating role of green consumer values in the relationship between green marketing and consumer behaviour. The study focused on the important role of green consumer values in shaping environmentally conscious consumer actions, emphasizing the importance of considering these values in green marketing strategies.To achieve the aim, the study adopted a positivist paradigm, quantitative approach, and a causal research design. A survey technique was employed to gather primary data where a self-administered questionnaire was issued to 450 respondents who were purposefully sampled. The study’s sample constituted consumers that frequently visit hotels in the Eastern Cape. The key findings of the study suggest that there is a significant role that green marketing plays in consumer behaviour towards green products offered in hotels. Furthermore, the findings highlight how green consumer values influence consumers towards value-driven choices for sustainable practices in the hotel industry, high level of satisfaction and preference for green products. Based on the findings, government and the hospitality industry should implement policies that will provide incentives for hotels that practise sustainable measures. This could include free quarterly advertisement of green hotels, free hampers for consumers that make use of green hotels on a regularly basis, and a spa voucher for every new consumer. Government should also provide capital and resources to small businesses to upscale their establishments to meet green hotel standards, for instance provide funding for solar installation and for those who already have installed solar panels to be reimbursed a fraction of the amount they paid.This study makes a significant contribution by highlighting the important role of green consumer values as a moderator. It reveals how these values impact the relationship between green marketing strategies and consumer behaviour. The study provides valuable insights for marketers, emphasizing the need to align strategies with green consumer values to promote more effective and impactful responses from consumers towards environmentally friendly initiatives. , Thesis (MA) -- Faculty of Economic and Financial Sciences, Business Management and Economics, 2024
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Exclusive breastfeeding up to six months in very and extremly low birth weight infants and determinants of breastfeeding practices at the Frere Hospital in the Eastern Cape, South Africa
- Authors: Haughton, Anna-Marie
- Date: 2024-03
- Subjects: Brestfeeding Practices
- Language: English
- Type: Masters , text
- Identifier: http://hdl.handle.net/11260/11065 , vital:75881
- Description: INTRODUCTION: Breastmilk provides adequate nutrition for the optimal growth and development of infants globally. Breastfeeding is an incomparable practice that cannot sufficiently be replaced by any other substance. Breastmilk and breastfeeding offer numerous medical and non-medical advantages that influence the welfare not only of infants, but also of women, their families and society. The last few decades have shown a worldwide increase in the birth and survival rate of preterm, very low birth weight (VLBW) and extremely low birth weight (ELBW) infants. These infants have an increased risk of adverse outcomes, thus necessitating optimal management including best feeding practices of this vulnerable population. AIM: This study assessed the exclusive breastfeeding (EBF) rate in VLBW and ELBW infants at their corrected six months of age and aimed to identify reasons for premature cessation of EBF as well as appraising the association between maternal socio-demographic variables and breastfeeding practices. This study also investigated the type, volume and preparation of replacement feeds given to infants that were not EBF. iii METHOD: This retrospective, cross sectional descriptive study was conducted between December 2017 to November 2018 at Frere Hospital in the Eastern Cape. A comprehensive database was compiled with all the infants’ demographics and relevant maternal socio-demographic variables at birth. Information on the changeover of primary caregiver, feeding practices, reasons stated for premature discontinuation of EBF, and the infant’s anthropometry were documented at each follow up visit and included in the database. RESULTS: The prevalence of EBF at the corrected six month follow up was 22%. Insufficient breastmilk supply (23%, 43% and 13% at one, three and six months respectively) and maternal commitments to school or work (35%, 33% and 50% at one, three and six months respectively) were the predominant reasons stated by mothers for discontinuation of EBF. The number of other children a mother had was significantly related to EBF practices at months one and six (p = .029 and .025, respectively), and EBF was also significantly related to the level of maternal education at month three (p = .006). The percentage of mothers who breastfed exclusively was proportional to the number of other children they had, and inversely proportional to the level of their education. The volume of replacement feeds was known to be incorrect in over a third of cases at months one and three (35% and 34%, respectively), and in almost half of cases at month six (48%). Replacement feeds were being prepared most incorrectly at the three month follow up with almost half (46%) of these feeds being prepared iv inaccurately. These replacement feeds comprised of exclusive formula feeds (EFF) for 95% of cases that were not EBF at month one, 88% at month three, and 97% at month six. CONCLUSIONS: The prevalence of EBF in VLBW and ELBW infants at six months in this study remains sub-optimal compared with other low- and middle-income countries (LMICs) and is much lower than the target set by the World Health Organisation (WHO). Factors that affected EBF practices in this study include mother’s perception of insufficient breastmilk supply, maternal commitments to work/school, the number of other children in the household as well as the level of maternal education. A combination of various interventions is urgently needed to effectively increase EBF rates in this population. , Thesis (Masters) -- Faculty of Medicine and Health Sciences, 2024
- Full Text:
- Authors: Haughton, Anna-Marie
- Date: 2024-03
- Subjects: Brestfeeding Practices
- Language: English
- Type: Masters , text
- Identifier: http://hdl.handle.net/11260/11065 , vital:75881
- Description: INTRODUCTION: Breastmilk provides adequate nutrition for the optimal growth and development of infants globally. Breastfeeding is an incomparable practice that cannot sufficiently be replaced by any other substance. Breastmilk and breastfeeding offer numerous medical and non-medical advantages that influence the welfare not only of infants, but also of women, their families and society. The last few decades have shown a worldwide increase in the birth and survival rate of preterm, very low birth weight (VLBW) and extremely low birth weight (ELBW) infants. These infants have an increased risk of adverse outcomes, thus necessitating optimal management including best feeding practices of this vulnerable population. AIM: This study assessed the exclusive breastfeeding (EBF) rate in VLBW and ELBW infants at their corrected six months of age and aimed to identify reasons for premature cessation of EBF as well as appraising the association between maternal socio-demographic variables and breastfeeding practices. This study also investigated the type, volume and preparation of replacement feeds given to infants that were not EBF. iii METHOD: This retrospective, cross sectional descriptive study was conducted between December 2017 to November 2018 at Frere Hospital in the Eastern Cape. A comprehensive database was compiled with all the infants’ demographics and relevant maternal socio-demographic variables at birth. Information on the changeover of primary caregiver, feeding practices, reasons stated for premature discontinuation of EBF, and the infant’s anthropometry were documented at each follow up visit and included in the database. RESULTS: The prevalence of EBF at the corrected six month follow up was 22%. Insufficient breastmilk supply (23%, 43% and 13% at one, three and six months respectively) and maternal commitments to school or work (35%, 33% and 50% at one, three and six months respectively) were the predominant reasons stated by mothers for discontinuation of EBF. The number of other children a mother had was significantly related to EBF practices at months one and six (p = .029 and .025, respectively), and EBF was also significantly related to the level of maternal education at month three (p = .006). The percentage of mothers who breastfed exclusively was proportional to the number of other children they had, and inversely proportional to the level of their education. The volume of replacement feeds was known to be incorrect in over a third of cases at months one and three (35% and 34%, respectively), and in almost half of cases at month six (48%). Replacement feeds were being prepared most incorrectly at the three month follow up with almost half (46%) of these feeds being prepared iv inaccurately. These replacement feeds comprised of exclusive formula feeds (EFF) for 95% of cases that were not EBF at month one, 88% at month three, and 97% at month six. CONCLUSIONS: The prevalence of EBF in VLBW and ELBW infants at six months in this study remains sub-optimal compared with other low- and middle-income countries (LMICs) and is much lower than the target set by the World Health Organisation (WHO). Factors that affected EBF practices in this study include mother’s perception of insufficient breastmilk supply, maternal commitments to work/school, the number of other children in the household as well as the level of maternal education. A combination of various interventions is urgently needed to effectively increase EBF rates in this population. , Thesis (Masters) -- Faculty of Medicine and Health Sciences, 2024
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Bioprospectin of medicinal cannabis from Eastern Cape, South Africa
- Authors: Odieka, Anwuli, Endurance
- Date: 2024-02
- Subjects: Organic chemistry
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/11260/9427 , vital:73532
- Description: Cannabis sativa L. (Family Cannabaceae) has continued to attract much interest among natural product scientists in recent time because of its known medicinal values and remarkable chemical diversity. This study reports the investigation of C. sativa cultivated in the Eastern Cape Province of South Africa, with a view to obtaining its chemical fingerprint and isolating compounds from its stem bark for possible biological applications. Fresh and dried leaves, flowers, stem barks and roots of two Cannabis cultivars were obtained under permit NO. PIA-HP-EC-2022-0023. The fresh and dried plant materials were extracted for their essential oils (EOs), using hydro-distillation method on a Clevenger apparatus set at optimum extraction condition. The EOs were characterized by a gas chromatography coupled to a mass spectrometer (GC-MS). The chemical components of the EOs were elucidated based on their retention indices, as well as with reference to literature report. Other portions of the dried leaf, inflorescence and stem bark of each cultivar were extracted with methanol. The methanol extract was profiled for its cannabinoid content using liquid chromatography mass spectrometry (LCMS) based metabolomics approach, with reference to standard cannabinoids (∆9-THC, ∆9-THCA, and CBD). Lastly, the dried stem bark of Cultivar II was investigated for its compounds. The stem bark powder was subjected to sequential extraction at room temperature with n-hexane, dichloromethane, ethyl acetate and methanol. Based on the TLC analysis, the afforded n-hexane and ethyl acetate extracts were further individually fractionated on a repeated column chromatography, using silica gel and Sephadex LH20 as stationary phases. The column fractionation process led to the isolation of seven compounds, E1-E4, A20, A21, and A23. The isolated compounds were characterized by spectroscopic and spectrometric methods on nuclear magnetic resonance (NMR) spectrometer and ultra high-performance liquid chromatography hyphenated to a mass spectrometer, respectively. The results from the study showed that the EOs of the C. sativa cultivars contain majorly sesquiterpenes in their oxygenated and non-oxygenated forms. Caryophyllene (0.6421.4%), caryophyllene oxide (1.27-20.34%), -pinene (0.35-13.21%), D-limonene (0.51-10.99%), humulene (0.37-10.56%), humulene-1,2-epoxide (0.45-5.98%), pinene (0.16-5.22%), nerolidol (0.32-4.99%), -myrcene (0.31-4.23%), -selinene (0.92-2.51%), and trans--bergamotene (0.50-2.70%), were the main terpene constituents of the EOs in both cultivars. Neutral cannabinoids were also detected in both cultivars with cannabidiol found in significant amount up to 85.03% in the fresh stem oil (LSO) of Cultivar I (Lifter). LC-MS fingerprints revealed that Cultivar I had the highest accumulation of cannabinoids with CBDA having the highest amount (88.65%) in the stembark and CBD at about 23% in the leaves. The inflorescence (flower) contained 51.55% of CBDA. Cultivar II had the least number of cannabinoids but contains the highest amount of ∆9-THCA (94.8%) in the leaf sample. Cultivar II showed ∆9-THCA and ∆9-THC dominant profiles with less than 2% of ∆8-THC. ∆9-THC, ∆8- THC were the only neutral cannabinoids detected in Cultivar II while CBD, ∆9-THC, and ∆8- THC were found in significant amounts in Cultivar I. The compounds isolated from the stem bark of Cultivar II were elucidated as five triterpenoids: Sativalenic acid ethyl ester (E1), Phyllanthone (E2), Friedelin (E3), Sativalonone (A20) and Sativaol (A23); an alkaloid, Sativacherine (E4); and a fatty acid ester, Ethyl stearate (A21). Four of these compounds are newly described, while all the compounds are reported for the first time from the Eastern Cape Cultivar (Cherrywine) of C. sativa stem. , Thesis (PHD) -- Faculty of Natural Sciences, 2024
- Full Text:
- Authors: Odieka, Anwuli, Endurance
- Date: 2024-02
- Subjects: Organic chemistry
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/11260/9427 , vital:73532
- Description: Cannabis sativa L. (Family Cannabaceae) has continued to attract much interest among natural product scientists in recent time because of its known medicinal values and remarkable chemical diversity. This study reports the investigation of C. sativa cultivated in the Eastern Cape Province of South Africa, with a view to obtaining its chemical fingerprint and isolating compounds from its stem bark for possible biological applications. Fresh and dried leaves, flowers, stem barks and roots of two Cannabis cultivars were obtained under permit NO. PIA-HP-EC-2022-0023. The fresh and dried plant materials were extracted for their essential oils (EOs), using hydro-distillation method on a Clevenger apparatus set at optimum extraction condition. The EOs were characterized by a gas chromatography coupled to a mass spectrometer (GC-MS). The chemical components of the EOs were elucidated based on their retention indices, as well as with reference to literature report. Other portions of the dried leaf, inflorescence and stem bark of each cultivar were extracted with methanol. The methanol extract was profiled for its cannabinoid content using liquid chromatography mass spectrometry (LCMS) based metabolomics approach, with reference to standard cannabinoids (∆9-THC, ∆9-THCA, and CBD). Lastly, the dried stem bark of Cultivar II was investigated for its compounds. The stem bark powder was subjected to sequential extraction at room temperature with n-hexane, dichloromethane, ethyl acetate and methanol. Based on the TLC analysis, the afforded n-hexane and ethyl acetate extracts were further individually fractionated on a repeated column chromatography, using silica gel and Sephadex LH20 as stationary phases. The column fractionation process led to the isolation of seven compounds, E1-E4, A20, A21, and A23. The isolated compounds were characterized by spectroscopic and spectrometric methods on nuclear magnetic resonance (NMR) spectrometer and ultra high-performance liquid chromatography hyphenated to a mass spectrometer, respectively. The results from the study showed that the EOs of the C. sativa cultivars contain majorly sesquiterpenes in their oxygenated and non-oxygenated forms. Caryophyllene (0.6421.4%), caryophyllene oxide (1.27-20.34%), -pinene (0.35-13.21%), D-limonene (0.51-10.99%), humulene (0.37-10.56%), humulene-1,2-epoxide (0.45-5.98%), pinene (0.16-5.22%), nerolidol (0.32-4.99%), -myrcene (0.31-4.23%), -selinene (0.92-2.51%), and trans--bergamotene (0.50-2.70%), were the main terpene constituents of the EOs in both cultivars. Neutral cannabinoids were also detected in both cultivars with cannabidiol found in significant amount up to 85.03% in the fresh stem oil (LSO) of Cultivar I (Lifter). LC-MS fingerprints revealed that Cultivar I had the highest accumulation of cannabinoids with CBDA having the highest amount (88.65%) in the stembark and CBD at about 23% in the leaves. The inflorescence (flower) contained 51.55% of CBDA. Cultivar II had the least number of cannabinoids but contains the highest amount of ∆9-THCA (94.8%) in the leaf sample. Cultivar II showed ∆9-THCA and ∆9-THC dominant profiles with less than 2% of ∆8-THC. ∆9-THC, ∆8- THC were the only neutral cannabinoids detected in Cultivar II while CBD, ∆9-THC, and ∆8- THC were found in significant amounts in Cultivar I. The compounds isolated from the stem bark of Cultivar II were elucidated as five triterpenoids: Sativalenic acid ethyl ester (E1), Phyllanthone (E2), Friedelin (E3), Sativalonone (A20) and Sativaol (A23); an alkaloid, Sativacherine (E4); and a fatty acid ester, Ethyl stearate (A21). Four of these compounds are newly described, while all the compounds are reported for the first time from the Eastern Cape Cultivar (Cherrywine) of C. sativa stem. , Thesis (PHD) -- Faculty of Natural Sciences, 2024
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Knowledge, attitudes and perceptions towards hand hygine practice amongst students at a nursing colledge, Lesotho
- Authors: Ntaote, Malehlohonolo
- Date: 2024-02
- Language: English
- Type: Masters theses , text
- Identifier: http://hdl.handle.net/11260/13000 , vital:76187
- Description: Hands are critical vectors for microorganisms that cause hospital acquired infections, hand hygiene, being done the right way, at the right time and being given the right priority at health care setting is an effective strategy to reduce hospital acquired infections and costs. Different strategies have been put in place to help improve hand hygiene compliance among health care workers, amongst them; continuous training is advised at all levels of care. This study aimed to describe the knowledge, attitudes and perceptions towards hand hygiene in nursing students in Maluti College Berea Lesotho Methods This study was a quantitative cross-sectional study; 114 questionnaires were distributed and 103 were filled and returned by available willing students in Maluti College, that was 90% response rate. Results Females were more than males at 88% (91). Age range of the respondents were 18-35 years, with a mean of 22 years, 57% (59) were between ages 20-24 years. The students were distributed in four different class levels, lowest was third years at 19% (19) and highest was the nurse midwives at 32% (33). 75% (77) had hand hygiene training in the past three years. 62% (64) had moderate knowledge, all had positive attitudes and 61% (63) had a positive perception towards hand hygiene. There was a significant (p value=0.012) association between knowledge and training. Conclusion and recommendations The study has demonstrated a significant association between knowledge and training, indicating the pivotal role of education in shaping practices related to hand hygiene among healthcare workers. These findings underscore the importance of integrating insights garnered from this study into the existing teaching curriculum for healthcare 3 professionals. Furthermore, the results provide valuable input for enhancing the World Health Organization's (WHO) hand hygiene multimodal strategy, emphasizing the need for targeted interventions that address knowledge and training gaps. Additionally, the study findings advocate for incorporation of evidence-based practices into initiatives such as the SAFE LIFE Clean YOUR HAND campaign, thereby reinforcing the importance of continuous education and training to promote effective hand hygiene practices and mitigate the spread of healthcare-associated infections. This calls for a concerted effort from policymakers, healthcare institutions, and educational stakeholders to implement strategies that prioritize knowledge dissemination and training programs to improve hand hygiene practices among healthcare workers worldwide. , Thesis (Masters) -- Faculty of Medicine and Health Sciences, 2024
- Full Text:
- Authors: Ntaote, Malehlohonolo
- Date: 2024-02
- Language: English
- Type: Masters theses , text
- Identifier: http://hdl.handle.net/11260/13000 , vital:76187
- Description: Hands are critical vectors for microorganisms that cause hospital acquired infections, hand hygiene, being done the right way, at the right time and being given the right priority at health care setting is an effective strategy to reduce hospital acquired infections and costs. Different strategies have been put in place to help improve hand hygiene compliance among health care workers, amongst them; continuous training is advised at all levels of care. This study aimed to describe the knowledge, attitudes and perceptions towards hand hygiene in nursing students in Maluti College Berea Lesotho Methods This study was a quantitative cross-sectional study; 114 questionnaires were distributed and 103 were filled and returned by available willing students in Maluti College, that was 90% response rate. Results Females were more than males at 88% (91). Age range of the respondents were 18-35 years, with a mean of 22 years, 57% (59) were between ages 20-24 years. The students were distributed in four different class levels, lowest was third years at 19% (19) and highest was the nurse midwives at 32% (33). 75% (77) had hand hygiene training in the past three years. 62% (64) had moderate knowledge, all had positive attitudes and 61% (63) had a positive perception towards hand hygiene. There was a significant (p value=0.012) association between knowledge and training. Conclusion and recommendations The study has demonstrated a significant association between knowledge and training, indicating the pivotal role of education in shaping practices related to hand hygiene among healthcare workers. These findings underscore the importance of integrating insights garnered from this study into the existing teaching curriculum for healthcare 3 professionals. Furthermore, the results provide valuable input for enhancing the World Health Organization's (WHO) hand hygiene multimodal strategy, emphasizing the need for targeted interventions that address knowledge and training gaps. Additionally, the study findings advocate for incorporation of evidence-based practices into initiatives such as the SAFE LIFE Clean YOUR HAND campaign, thereby reinforcing the importance of continuous education and training to promote effective hand hygiene practices and mitigate the spread of healthcare-associated infections. This calls for a concerted effort from policymakers, healthcare institutions, and educational stakeholders to implement strategies that prioritize knowledge dissemination and training programs to improve hand hygiene practices among healthcare workers worldwide. , Thesis (Masters) -- Faculty of Medicine and Health Sciences, 2024
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Advancing the scholarship of integration for impactful, sustainable and holistic student success
- Pule, Neo, Madiba, Matete, Mohasoa, Irene
- Authors: Pule, Neo , Madiba, Matete , Mohasoa, Irene
- Date: 2024
- Language: English
- Type: Article
- Identifier: http://hdl.handle.net/11260/13055 , vital:77162 , ISSN: 2307-6267 , Citation: Pule, N., Madiba, M., & Mohasoa, I. (2024). Advancing the scholarship of integration for impactful, sustainable and holistic student success. Journal of Student Affairs in Africa, 12(2), v–ix. DOI: 10.24085/jsaa.v12i2.5483. , DOI: 10.24085/jsaa.v12i2.5483
- Description: Abstract: University professional and teaching staff are like two sides of the same coin. However, to a great extent, they work in silos – which leads to fragmentation and undermines efforts to achieve significant levels of impactful, sustainable and holistic student success. In this context, this special issue was proposed as a contribution towards closing the gap between the academic part of the university and the professional or support-services part; and as a means of encouraging the work required to forge integrated approaches in support of enhanced student success. The siloed approach, which is indicative of the politics and potential power dynamics that are embedded in university operations and structures, needs to be dismantled...
- Full Text:
- Authors: Pule, Neo , Madiba, Matete , Mohasoa, Irene
- Date: 2024
- Language: English
- Type: Article
- Identifier: http://hdl.handle.net/11260/13055 , vital:77162 , ISSN: 2307-6267 , Citation: Pule, N., Madiba, M., & Mohasoa, I. (2024). Advancing the scholarship of integration for impactful, sustainable and holistic student success. Journal of Student Affairs in Africa, 12(2), v–ix. DOI: 10.24085/jsaa.v12i2.5483. , DOI: 10.24085/jsaa.v12i2.5483
- Description: Abstract: University professional and teaching staff are like two sides of the same coin. However, to a great extent, they work in silos – which leads to fragmentation and undermines efforts to achieve significant levels of impactful, sustainable and holistic student success. In this context, this special issue was proposed as a contribution towards closing the gap between the academic part of the university and the professional or support-services part; and as a means of encouraging the work required to forge integrated approaches in support of enhanced student success. The siloed approach, which is indicative of the politics and potential power dynamics that are embedded in university operations and structures, needs to be dismantled...
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Building sustainable economies: Navigating contemporary issues in the finance, economic complexity, and interprenuership development
- Authors: Ncanywa, Thobeka
- Date: 2024
- Subjects: Contemporary issues in the finance--economic complexity--interprenuership development Finace--Economics--Interprenuership
- Language: English
- Type: text , Lectures
- Identifier: http://hdl.handle.net/11260/9587 , vital:74596
- Full Text:
- Authors: Ncanywa, Thobeka
- Date: 2024
- Subjects: Contemporary issues in the finance--economic complexity--interprenuership development Finace--Economics--Interprenuership
- Language: English
- Type: text , Lectures
- Identifier: http://hdl.handle.net/11260/9587 , vital:74596
- Full Text:
Building sustainable economies: Navigating contemporary issues in the finance, economic complexity, and interprenuership development
- Authors: Ncanywa, Thobeka
- Date: 2024
- Subjects: Contemporary issues in the finance--economic complexity--interprenuership development Finace--Economics--Interprenuership
- Language: English
- Type: text , Lectures
- Identifier: http://hdl.handle.net/11260/9607 , vital:74597
- Full Text:
- Authors: Ncanywa, Thobeka
- Date: 2024
- Subjects: Contemporary issues in the finance--economic complexity--interprenuership development Finace--Economics--Interprenuership
- Language: English
- Type: text , Lectures
- Identifier: http://hdl.handle.net/11260/9607 , vital:74597
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Emergency Obstetric Operating Theatre Efficiency at a Tertiary Level Hospital in the Eastern Cape
- Authors: Burger, W H
- Date: 2024
- Subjects: Operating theatres , Emergency obstetrics theatres , Academic hospitals , Eastern Cape
- Language: English
- Type: Masters (Thesis)
- Identifier: http://hdl.handle.net/11260/13522 , vital:78942
- Description: Operating theatres account for large proportions of health care costs, and the misuse of allocated theatre time results in wasteful expenditure of resources that leads to compromised quality of health service delivery. It is crucial for health systems and hospitals to regularly examine theatre efficiency. This is especially relevant to emergency obstetrics theatres as inefficient use translates into prolonged patient waiting times and increases maternal and infant morbidity and mortality. There is a paucity of research on obstetrics operating theatre efficiency, especially in the Eastern Cape. , Degree: M. Science (Anaesthesia)
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- Authors: Burger, W H
- Date: 2024
- Subjects: Operating theatres , Emergency obstetrics theatres , Academic hospitals , Eastern Cape
- Language: English
- Type: Masters (Thesis)
- Identifier: http://hdl.handle.net/11260/13522 , vital:78942
- Description: Operating theatres account for large proportions of health care costs, and the misuse of allocated theatre time results in wasteful expenditure of resources that leads to compromised quality of health service delivery. It is crucial for health systems and hospitals to regularly examine theatre efficiency. This is especially relevant to emergency obstetrics theatres as inefficient use translates into prolonged patient waiting times and increases maternal and infant morbidity and mortality. There is a paucity of research on obstetrics operating theatre efficiency, especially in the Eastern Cape. , Degree: M. Science (Anaesthesia)
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Epidemiology and Outcomes of Cutaneous Drug Reactions In an HIV/TB Endemic Setting
- Konyana, Pumlani Pumelele Stephen
- Authors: Konyana, Pumlani Pumelele Stephen
- Date: 2024
- Subjects: Cutaneous drug reactions , HIV/TB endemic settings , Severe cutaneous adverse drug reactions
- Language: English
- Type: Masters (Thesis)
- Identifier: http://hdl.handle.net/11260/13427 , vital:78867
- Description: Cutaneous drug reactions (CDR) are the most common adverse drug reactions. The occurrence of CDR most often interrupts the treatment regimen. In HIV/TB endemic settings, severe CDR are more prevalent than mild or moderate forms. Treatment interruption as a result of severe cutaneous adverse drug reactions (SCAR) in HIV/TB patients compromises the efficacy of ARV and anti-TB drugs. Considering the current measure of treatment discontinuation in managing SCAR in HIV/TB patients, and the known effects on treatment outcomes, there is a need to carry out research of this kind in a typical HIV/TB endemic setting. , Degree: M. Medicine in Dermatology
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- Authors: Konyana, Pumlani Pumelele Stephen
- Date: 2024
- Subjects: Cutaneous drug reactions , HIV/TB endemic settings , Severe cutaneous adverse drug reactions
- Language: English
- Type: Masters (Thesis)
- Identifier: http://hdl.handle.net/11260/13427 , vital:78867
- Description: Cutaneous drug reactions (CDR) are the most common adverse drug reactions. The occurrence of CDR most often interrupts the treatment regimen. In HIV/TB endemic settings, severe CDR are more prevalent than mild or moderate forms. Treatment interruption as a result of severe cutaneous adverse drug reactions (SCAR) in HIV/TB patients compromises the efficacy of ARV and anti-TB drugs. Considering the current measure of treatment discontinuation in managing SCAR in HIV/TB patients, and the known effects on treatment outcomes, there is a need to carry out research of this kind in a typical HIV/TB endemic setting. , Degree: M. Medicine in Dermatology
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Factors influencing digital marketing adoption for female interpreneurs in small and medium enterprices of OR Thambo Municipality
- Authors: Maduna, Siyasanga
- Date: 2024
- Subjects: Digital Marketing
- Language: English
- Type: Masters theses , text
- Identifier: http://hdl.handle.net/11260/10065 , vital:74935
- Description: The rise of the Internet has transformed the business landscape, leading to the rapid growth of major online retailers and service providers like Amazon, Facebook, and Google, all of which play significant roles in the digital economy (Kannan, 2017). Global internet usage continues to grow, with South Africa not an exception. The research seeks to explore the factors influencing digital marketing adoption for female entrepreneurs on small and medium enterprise in OR Tambo municipality. This dissertation employs a quantitative research design. The study employs structural equation modelling (SEM) techniques to analyse a comprehensive dataset gathered from 313 female entrepreneurs. The findings reveal a nuanced relationship between adoption of digital marketing and business performance. The SEM results indicate that H4, have a positive influence on adoption of digital marketing, and H5, have a positive influence on business performance. The hypotheses H1; H2 and H3 had negative relationship on adoption on adoption of digital marketing hence they were rejection. The implications of these findings are discussed in the context of factors that influence the adoption of digital marketing on enhancing business performance for SME’s. The dissertation contributes to inclusive economic development, policy implications; educational interventions; strategic business imperative and social impact. Beyond the economic aspects, the study contributes to social impact by challenging and potentially reshaping societal norms and expectations regarding women's roles in business. This research not only advances the theoretical understanding of the link between adoption of digital marketing and business performance but also provides valuable insights for designing effective marketing strategies to improve business performance on female entrepreneurs. The dissertation concludes with suggestions for future research directions to further refine an understanding of the intricate dynamics involved in the relationship between adoption of digital marketing and business performance. , Thesis (Masters) -- Faculty of Economics and Financial Sciences, 2024
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- Authors: Maduna, Siyasanga
- Date: 2024
- Subjects: Digital Marketing
- Language: English
- Type: Masters theses , text
- Identifier: http://hdl.handle.net/11260/10065 , vital:74935
- Description: The rise of the Internet has transformed the business landscape, leading to the rapid growth of major online retailers and service providers like Amazon, Facebook, and Google, all of which play significant roles in the digital economy (Kannan, 2017). Global internet usage continues to grow, with South Africa not an exception. The research seeks to explore the factors influencing digital marketing adoption for female entrepreneurs on small and medium enterprise in OR Tambo municipality. This dissertation employs a quantitative research design. The study employs structural equation modelling (SEM) techniques to analyse a comprehensive dataset gathered from 313 female entrepreneurs. The findings reveal a nuanced relationship between adoption of digital marketing and business performance. The SEM results indicate that H4, have a positive influence on adoption of digital marketing, and H5, have a positive influence on business performance. The hypotheses H1; H2 and H3 had negative relationship on adoption on adoption of digital marketing hence they were rejection. The implications of these findings are discussed in the context of factors that influence the adoption of digital marketing on enhancing business performance for SME’s. The dissertation contributes to inclusive economic development, policy implications; educational interventions; strategic business imperative and social impact. Beyond the economic aspects, the study contributes to social impact by challenging and potentially reshaping societal norms and expectations regarding women's roles in business. This research not only advances the theoretical understanding of the link between adoption of digital marketing and business performance but also provides valuable insights for designing effective marketing strategies to improve business performance on female entrepreneurs. The dissertation concludes with suggestions for future research directions to further refine an understanding of the intricate dynamics involved in the relationship between adoption of digital marketing and business performance. , Thesis (Masters) -- Faculty of Economics and Financial Sciences, 2024
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Farmers’ perceptions of storage pests and the efficacy of Citrus clementina waste products on stored maize
- Authors: Gumede, Bongumusa Charles
- Date: 2024
- Subjects: Posthavest , Smallholder farmers , Citrus clementina , Sitophilus zeamais , Botanical pesticides
- Language: English
- Type: Masters (Thesis)
- Identifier: http://hdl.handle.net/11260/13452 , vital:78932
- Description: Maize weevil is a major economic pest of stored maize in most rural communities. Infestations by this insect pest begins in the field but severe damage occur during storage. This study aims to document smallholder farmers’ knowledge of storage insect pests, their current control practices and assess insecticidal efficacy of Citrus clementina Hort ex Tan leaf and peel powder against maize weevil on stored maize. Semi-structured questionnaires were administered to 140 smallholder farmers in King Sabata Dalindyebo Local Municipality and Nyandeni Local Municipality under Oliver Reginald Tambo District municipality in Eastern Cape Province of South Africa. Results indicated that metal tanks tend to be the most preferred (70.28%) storage facility for storing maize and Sitophilus zeamais Motschulsky and Sitotroga cerealella (Olivier) were the major storage insect pests of maize. Farmers reported managing these pests using chemical pesticide (84.28%) namely aluminium phosphide. To minimize overreliance on synthetic pesticides, there is need to find alternative methods that are safe to supplement this current method. Hence, the current study tested the effectiveness of C. clementina peel and leaf powder as a biopesticide in managing maize weevils on stored maize. The maize grain mixed with different amounts of C. clementina peel and leaf powder in the metal containers were assessed monthly for 5 months by observing grains under the microscope to monitor damage. This was followed by opening the grains to inspect for the presence or absence of any developmental stage of the maize weevil. The C. clementina powder was found to be effective in suppressing the population of emerging maize weevils in maize that was infested in the farm. Thus, the findings of this study indicate that C. clementina could serve as a safer alternative to synthetic insecticides in suppressing emerging S. zeamais in stored maize grains. , Degree: M.Science (Zoology)
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- Authors: Gumede, Bongumusa Charles
- Date: 2024
- Subjects: Posthavest , Smallholder farmers , Citrus clementina , Sitophilus zeamais , Botanical pesticides
- Language: English
- Type: Masters (Thesis)
- Identifier: http://hdl.handle.net/11260/13452 , vital:78932
- Description: Maize weevil is a major economic pest of stored maize in most rural communities. Infestations by this insect pest begins in the field but severe damage occur during storage. This study aims to document smallholder farmers’ knowledge of storage insect pests, their current control practices and assess insecticidal efficacy of Citrus clementina Hort ex Tan leaf and peel powder against maize weevil on stored maize. Semi-structured questionnaires were administered to 140 smallholder farmers in King Sabata Dalindyebo Local Municipality and Nyandeni Local Municipality under Oliver Reginald Tambo District municipality in Eastern Cape Province of South Africa. Results indicated that metal tanks tend to be the most preferred (70.28%) storage facility for storing maize and Sitophilus zeamais Motschulsky and Sitotroga cerealella (Olivier) were the major storage insect pests of maize. Farmers reported managing these pests using chemical pesticide (84.28%) namely aluminium phosphide. To minimize overreliance on synthetic pesticides, there is need to find alternative methods that are safe to supplement this current method. Hence, the current study tested the effectiveness of C. clementina peel and leaf powder as a biopesticide in managing maize weevils on stored maize. The maize grain mixed with different amounts of C. clementina peel and leaf powder in the metal containers were assessed monthly for 5 months by observing grains under the microscope to monitor damage. This was followed by opening the grains to inspect for the presence or absence of any developmental stage of the maize weevil. The C. clementina powder was found to be effective in suppressing the population of emerging maize weevils in maize that was infested in the farm. Thus, the findings of this study indicate that C. clementina could serve as a safer alternative to synthetic insecticides in suppressing emerging S. zeamais in stored maize grains. , Degree: M.Science (Zoology)
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