Towards understanding the effect of size variation on the aggressive and feeding behaviours of juvenile dusky kob Argyrosomus japonicus (Pisces: Sciaenidae)
- Authors: Babane, Siviwe Elvis
- Date: 2018
- Subjects: Argyrosomus -- Feeding and feeds , Aquaculture , Fishes -- Cannibalism , Fishes -- Feeding and feeds
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/59592 , vital:27629
- Description: Many studies have been conducted on the effect of size-grading in other fish species. However, there is a paucity of scientific information on the effects of size variation on cannibalism of juvenile dusky kob. Thus, a study focusing on the effect of size variation on juvenile dusky kob aggressive and feeding (browsing) behaviours was conducted. Three separate groups of hatchery-reared juvenile dusky kob of were obtained from Oceanwise (Pty) Ltd for use in a series of three experimental trials. In all trials, juvenile fish of the same age were size-graded and the COV-value was used to determine the size variation. The focal fish (largest fish) was exposed to groups of fish with different size variation for 30-min. behavioural observations before and after feeding in randomised trials. The first experimental trial (Chapter 2) quantified the effect of increasing size variation and observation time on the aggressive and browsing behaviours of juvenile dusky kob. On average, juvenile dusky kob weighed 3.60 ± 0.68 g fish-1 and measured 5.8 ± 0.41 mm. Each focal fish was observed (a) before feeding in the morning, (b) 2 h after feeding, (c) 6 h after feeding and (d) 12 h after feeding. Fish increased browsing behaviours (averaging 6.60 ± 0.56) and decreased intimidating aggressive behaviours (18.60 ± 1.39) 12 h after feeding. Other aggressive behaviours occurred but did not differ between observation times. Aggressive and browsing behaviours positively correlated with size variation variables, predominantly, 12 h after feeding. An average frequency of 19 chases were observed positively correlated with size variation, followed by average frequencies of 17 body bites and browses, and 11 tail bites per 30 min. Some behaviours including average frequencies of 0.2 chases, 4 tail bites, 2.4 intimidating and 0.3 browsing behaviours negatively correlated with the size variation, generally closer to the last meal. These preliminary observations thus showed that fish have become hungry approximately 6-12 h after feeding and substituted certain behaviours for others as time after feeding passed and as size variation increased. The second experimental trial (Chapter 3) consisted of the observations further testing the relationship between aggressive behaviours and size variation of juveniles of dusky kob averaging 0.43 ± 0.27 g fish-1. The focal fish was exposed to groups of fish of four size variation (COV) treatments for observations before and 12 h after feeding. Aggressive behaviours positively correlated with size variation both before and 12 h after feeding. An average frequency of 437 body bites positively correlated more often with COV, followed by average frequencies of 365 intimidating behaviours and 199 tail bites per 30 min., respectively, before and 12 h after feeding. The least often exhibited aggressive behaviours averaged 26 chases while positively correlating with size variation on the times specified. An average frequency of 311 intimidating behaviours before and after feeding including average of 28 tail bites after feeding negatively correlated with size variation. This may relate to shift of behaviours depending on the needs and capacity of the fish. Apparently, aggressive fish can change its behaviour as a function of COV-values rather than the mean size of the other fish. The third trial (Chapter 4) investigated the effect of the aggressor’s (focal fish) size in relation its aggressive and browsing behaviours to other fish (non-focal fish). Juvenile fish used for this trial, on average, weighed 30 ± 7.63 g fish-1. Aggressive and browsing behaviours were observed in four treatments of a) high COV and mean weight below, b) low COV and mean weight less, c) high COV and mean weight equivalent to and d) low COV and mean weight higher than that of the focal fish. Increased frequencies of aggressive and browsing behaviours per 30 min. occurred in treatment A, sharing similar frequencies in treatment C, compared to the other treatments (B and D) which shared certain frequencies. The intimidating behaviours predominated, followed by browsing, body bites, chases and tail bites, respectively. The results of the overall study suggest that the time passed after feeding and increasing size variation and differences facilitated aggressive and browsing behaviours in juvenile dusky kob. Dusky kob showed increasing aggressive behaviours as early as in the first two weeks after hatching, averaging 0.43 ± 0.27 g fish-1 with the frequency correlating with size differences. Consistent size-grading technique in the same-age fish should be used to manage size variation associated with aggressive behaviours. The period of about 4-6 h after feeding may explain the noticeable increased aggressive and browsing behaviours. Thus, fish should be fed immediately before or after evacuation of their guts to maintain less-aggressive behaviours of juvenile dusky kob. Fish generally increased aggressive and browsing acts before and long time after feeding than closer to after feeding. The study has provided the fundamental scientific groundwork for fish farmers and future researchers can further explore size variation, time after feeding and gut evacuation rate as critical components of aggressive behaviours. The scientific knowledge of aggressive and cannibalistic behaviours has essential application in farming management to achieve improved survival and growth rates in juvenile fish.
- Full Text:
- Date Issued: 2018
- Authors: Babane, Siviwe Elvis
- Date: 2018
- Subjects: Argyrosomus -- Feeding and feeds , Aquaculture , Fishes -- Cannibalism , Fishes -- Feeding and feeds
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/59592 , vital:27629
- Description: Many studies have been conducted on the effect of size-grading in other fish species. However, there is a paucity of scientific information on the effects of size variation on cannibalism of juvenile dusky kob. Thus, a study focusing on the effect of size variation on juvenile dusky kob aggressive and feeding (browsing) behaviours was conducted. Three separate groups of hatchery-reared juvenile dusky kob of were obtained from Oceanwise (Pty) Ltd for use in a series of three experimental trials. In all trials, juvenile fish of the same age were size-graded and the COV-value was used to determine the size variation. The focal fish (largest fish) was exposed to groups of fish with different size variation for 30-min. behavioural observations before and after feeding in randomised trials. The first experimental trial (Chapter 2) quantified the effect of increasing size variation and observation time on the aggressive and browsing behaviours of juvenile dusky kob. On average, juvenile dusky kob weighed 3.60 ± 0.68 g fish-1 and measured 5.8 ± 0.41 mm. Each focal fish was observed (a) before feeding in the morning, (b) 2 h after feeding, (c) 6 h after feeding and (d) 12 h after feeding. Fish increased browsing behaviours (averaging 6.60 ± 0.56) and decreased intimidating aggressive behaviours (18.60 ± 1.39) 12 h after feeding. Other aggressive behaviours occurred but did not differ between observation times. Aggressive and browsing behaviours positively correlated with size variation variables, predominantly, 12 h after feeding. An average frequency of 19 chases were observed positively correlated with size variation, followed by average frequencies of 17 body bites and browses, and 11 tail bites per 30 min. Some behaviours including average frequencies of 0.2 chases, 4 tail bites, 2.4 intimidating and 0.3 browsing behaviours negatively correlated with the size variation, generally closer to the last meal. These preliminary observations thus showed that fish have become hungry approximately 6-12 h after feeding and substituted certain behaviours for others as time after feeding passed and as size variation increased. The second experimental trial (Chapter 3) consisted of the observations further testing the relationship between aggressive behaviours and size variation of juveniles of dusky kob averaging 0.43 ± 0.27 g fish-1. The focal fish was exposed to groups of fish of four size variation (COV) treatments for observations before and 12 h after feeding. Aggressive behaviours positively correlated with size variation both before and 12 h after feeding. An average frequency of 437 body bites positively correlated more often with COV, followed by average frequencies of 365 intimidating behaviours and 199 tail bites per 30 min., respectively, before and 12 h after feeding. The least often exhibited aggressive behaviours averaged 26 chases while positively correlating with size variation on the times specified. An average frequency of 311 intimidating behaviours before and after feeding including average of 28 tail bites after feeding negatively correlated with size variation. This may relate to shift of behaviours depending on the needs and capacity of the fish. Apparently, aggressive fish can change its behaviour as a function of COV-values rather than the mean size of the other fish. The third trial (Chapter 4) investigated the effect of the aggressor’s (focal fish) size in relation its aggressive and browsing behaviours to other fish (non-focal fish). Juvenile fish used for this trial, on average, weighed 30 ± 7.63 g fish-1. Aggressive and browsing behaviours were observed in four treatments of a) high COV and mean weight below, b) low COV and mean weight less, c) high COV and mean weight equivalent to and d) low COV and mean weight higher than that of the focal fish. Increased frequencies of aggressive and browsing behaviours per 30 min. occurred in treatment A, sharing similar frequencies in treatment C, compared to the other treatments (B and D) which shared certain frequencies. The intimidating behaviours predominated, followed by browsing, body bites, chases and tail bites, respectively. The results of the overall study suggest that the time passed after feeding and increasing size variation and differences facilitated aggressive and browsing behaviours in juvenile dusky kob. Dusky kob showed increasing aggressive behaviours as early as in the first two weeks after hatching, averaging 0.43 ± 0.27 g fish-1 with the frequency correlating with size differences. Consistent size-grading technique in the same-age fish should be used to manage size variation associated with aggressive behaviours. The period of about 4-6 h after feeding may explain the noticeable increased aggressive and browsing behaviours. Thus, fish should be fed immediately before or after evacuation of their guts to maintain less-aggressive behaviours of juvenile dusky kob. Fish generally increased aggressive and browsing acts before and long time after feeding than closer to after feeding. The study has provided the fundamental scientific groundwork for fish farmers and future researchers can further explore size variation, time after feeding and gut evacuation rate as critical components of aggressive behaviours. The scientific knowledge of aggressive and cannibalistic behaviours has essential application in farming management to achieve improved survival and growth rates in juvenile fish.
- Full Text:
- Date Issued: 2018
Apparent digestibility coefficients of feed ingredients and essential amino acid requirements of dusky kob (Argyrosomus japonicus)
- Authors: Adesola, Abidemi Adejoke
- Date: 2017
- Subjects: Amino acids in animal nutrition , Fishes -- Feeding and feeds , Argyrosomus japonicus , Argyrosomus , Argyrosomus japonicus -- Nutrition , Argyrosomus -- Nutrition , Argyrosomus japonicus -- Feeding and feeds , Argyrosomus -- Feeding and feeds
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/32140 , vital:24014
- Description: Important to the evaluation of potential feed ingredients for inclusion in fish diets should be their digestibility and amino acid requirement so that feeds can be formulated on a digestible basis rather than a gross nutrient basis. This thesis established techniques for faecal collection, the validity of digestibility markers, it determined apparent digestibility coefficients for various novel feed ingredients, and it established the optimal lysine requirement for Argyrosomus japonicus, which was used to estimate each of the essential amino acid requirements by using the ideal protein concept. There were no significant differences in dry matter apparent digestibility coefficients when faeces were collected by stripping (77.0 %), dissection (80.1 %) or settlement (83.5 %). Faecal collection by the settlement method produced the most reliable digestibility data. Apparent digestibility coefficients for dry matter, crude protein and energy calculated using acid-insoluble ash were higher (84.0 %, 93.2 % and 93.0 %, respectively) than those using chromic oxide (55.7 %, 79.1 % and 78.2 %) and titanium dioxide (58.6 %, 79.7 % and 80.5 %). The magnitude of variation in digestibility coefficients obtained using acid-insoluble ash was always lower than that obtained with the other markers. Therefore, acid-insoluble ash was preferred as a dietary marker. Using the above protocol, protein and amino acid digestibility of some animal and plant protein ingredients were evaluated in a series of experiments. The first trial compared the apparent digestibility coefficients of some animal products included as single protein source in the test diets. Apparent protein digestibility values were 84.5 %, 83.8 %, 85.8 % and 83.1 % for fishmeal-prime, fishmeal-standard, poultry meal and pork meal, respectively. Apparent digestibility coefficients for poultry meal were comparable to those of fishmeal, which indicate its potential as a substitute for fishmeal in the diets of A. japonicus. The second trial determined the apparent coefficients of plant and animal protein sources included at 30 % into a practical reference diet (70 %). Apparent protein digestibility ranged from 92.4 % in sunflower meal to 85.5 % in corn gluten meal. Soybean meal is a promising feed ingredient in A. japonicus due to the high apparent digestibility of its protein (92.0 %) and essential amino acid digestibility (mean average 91.4 %). A fundamental assumption in fish feed formulation is that the digestibility of nutrients is additive, i.e., digestibility of a nutrient in one ingredient does not interact with the digestibility of the same nutrient in another ingredient. In the third trial, additivity of feed ingredients was tested using pork meal and poultry meal. The results indicate that the apparent digestibility coefficient of animal protein ingredients could be calculated from compound diets to accurately determine protein and amino acid digestibility in A. japonicus, and possibly other carnivorous fish species. Dietary essential amino acid requirements were determined for juvenile A. japonicus in two trials. A dose-response study was conducted using crystalline lysine to determine the optimal requirement of dietary lysine for A. japonicus. Optimal dietary lysine was estimated at 31.7 g kg-¹ dry diet, corresponding to 73.5 g kg-¹ of dietary protein, based on specific growth rate and broken-line segmented regression analyses. Dietary requirements for other essential amino acids ranged from 22 g kg-¹ (histidine) to 71 g kg-¹ (leucine) crude protein. The results of the present study provided a research tool that could be used to assess and verify the conclusions of earlier dietary work on A. japonicus and in further studies to develop least cost diet formulations for this species. The study also adds to the knowledge of the nutritional requirements of A. japonicus by providing information on the digestibility of plants and animal protein ingredients. It also contributes to future dietary research for this species because this study determines, for the first time, the most suitable methods for investigating the digestibility of raw materials for A. japonicus.
- Full Text:
- Date Issued: 2017
- Authors: Adesola, Abidemi Adejoke
- Date: 2017
- Subjects: Amino acids in animal nutrition , Fishes -- Feeding and feeds , Argyrosomus japonicus , Argyrosomus , Argyrosomus japonicus -- Nutrition , Argyrosomus -- Nutrition , Argyrosomus japonicus -- Feeding and feeds , Argyrosomus -- Feeding and feeds
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/32140 , vital:24014
- Description: Important to the evaluation of potential feed ingredients for inclusion in fish diets should be their digestibility and amino acid requirement so that feeds can be formulated on a digestible basis rather than a gross nutrient basis. This thesis established techniques for faecal collection, the validity of digestibility markers, it determined apparent digestibility coefficients for various novel feed ingredients, and it established the optimal lysine requirement for Argyrosomus japonicus, which was used to estimate each of the essential amino acid requirements by using the ideal protein concept. There were no significant differences in dry matter apparent digestibility coefficients when faeces were collected by stripping (77.0 %), dissection (80.1 %) or settlement (83.5 %). Faecal collection by the settlement method produced the most reliable digestibility data. Apparent digestibility coefficients for dry matter, crude protein and energy calculated using acid-insoluble ash were higher (84.0 %, 93.2 % and 93.0 %, respectively) than those using chromic oxide (55.7 %, 79.1 % and 78.2 %) and titanium dioxide (58.6 %, 79.7 % and 80.5 %). The magnitude of variation in digestibility coefficients obtained using acid-insoluble ash was always lower than that obtained with the other markers. Therefore, acid-insoluble ash was preferred as a dietary marker. Using the above protocol, protein and amino acid digestibility of some animal and plant protein ingredients were evaluated in a series of experiments. The first trial compared the apparent digestibility coefficients of some animal products included as single protein source in the test diets. Apparent protein digestibility values were 84.5 %, 83.8 %, 85.8 % and 83.1 % for fishmeal-prime, fishmeal-standard, poultry meal and pork meal, respectively. Apparent digestibility coefficients for poultry meal were comparable to those of fishmeal, which indicate its potential as a substitute for fishmeal in the diets of A. japonicus. The second trial determined the apparent coefficients of plant and animal protein sources included at 30 % into a practical reference diet (70 %). Apparent protein digestibility ranged from 92.4 % in sunflower meal to 85.5 % in corn gluten meal. Soybean meal is a promising feed ingredient in A. japonicus due to the high apparent digestibility of its protein (92.0 %) and essential amino acid digestibility (mean average 91.4 %). A fundamental assumption in fish feed formulation is that the digestibility of nutrients is additive, i.e., digestibility of a nutrient in one ingredient does not interact with the digestibility of the same nutrient in another ingredient. In the third trial, additivity of feed ingredients was tested using pork meal and poultry meal. The results indicate that the apparent digestibility coefficient of animal protein ingredients could be calculated from compound diets to accurately determine protein and amino acid digestibility in A. japonicus, and possibly other carnivorous fish species. Dietary essential amino acid requirements were determined for juvenile A. japonicus in two trials. A dose-response study was conducted using crystalline lysine to determine the optimal requirement of dietary lysine for A. japonicus. Optimal dietary lysine was estimated at 31.7 g kg-¹ dry diet, corresponding to 73.5 g kg-¹ of dietary protein, based on specific growth rate and broken-line segmented regression analyses. Dietary requirements for other essential amino acids ranged from 22 g kg-¹ (histidine) to 71 g kg-¹ (leucine) crude protein. The results of the present study provided a research tool that could be used to assess and verify the conclusions of earlier dietary work on A. japonicus and in further studies to develop least cost diet formulations for this species. The study also adds to the knowledge of the nutritional requirements of A. japonicus by providing information on the digestibility of plants and animal protein ingredients. It also contributes to future dietary research for this species because this study determines, for the first time, the most suitable methods for investigating the digestibility of raw materials for A. japonicus.
- Full Text:
- Date Issued: 2017
The effect of carbohydrate on dusky kob, Argyrosomus japonicus, fed pelleted diets
- Authors: Mabasa, Nyiko Charity
- Date: 2017
- Subjects: Carbohydrates in animal nutrition , Fishes -- Feeding and feeds , Argyrosomus japonicus , Argyrosomus , Argyrosomus japonicus -- Nutrition , Argyrosomus -- Nutrition , Argyrosomus japonicus -- Feeding and feeds , Argyrosomus -- Feeding and feeds
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/44895 , vital:25450
- Description: The dusky kob, Argyrosomus japonicus, is an emerging commercial marine aquaculture species in South Africa. Attributes such as market acceptance, fast growth rates, tolerance to sibling density, acceptance of pelleted feed and adaptability to intensive aquaculture conditions make it a good candidate. Feed, the largest running cost in most aquaculture operations, is a limiting factor in dusky kob production as its nutritional requirements are not well understood. The present project formed part of a research program to develop a locally produced, least cost and sustainable feed that will support the growth and health of dusky kob. The approximate protein and energy requirements for kob fed formulated feeds have been established in earlier research, but the ability of kob to utilize carbohydrates as an energy source has not previously been investigated. The aim of this study was to determine the efficiency of dusky kob to utilize graded levels of carbohydrate (pregelatinized maize starch - PGMS) in pelleted diets. The research objectives were to determine the effect of dietary carbohydrate levels on:i) growth and feed utilization; ii) post prandial blood glucose levels; and iii) general fish health, gut bacterial composition and liver glycogen content of juvenile dusky kob. Hatchery reared juvenile dusky kob (5 g) were acclimatized in a recirculating experimental aquaculture system at the Department of Ichthyology and Fisheries Science at Rhodes University. They were fed trout crumble starter feed for three weeks before the start of the feeding experiment. Five diets containing 4.1, 8.2, 16.4 and 24.6% carbohydrate, hereafter referred to as 4.1C, 8.2C, 16.4C and 24.6C respectively, were formulated with pregelatinized maize starch as a carbohydrate source and fishmeal as the main protein source. Fish were fed these diets twice daily at 3.85% body weight per day for three months. Specific growth rates and feed conversion rations differed significantly between the four dietary treatments. Growth rate increased with increasing carbohydrate up to 16.4%, after which a significant decline was observed. Diet 16.4C produced the highest specific growth rate (SGR) of 1.84 ± 0.05% body weight/day, and the lowest feed conversion ratio of 1.28 ± 0.08. While the lowest specific growth rate and highest feed conversion ratio of 0.79 ± 0.05 and 2.14 ± 0.13 respectively, were found for diet 24.6C. A third order polynomial regression, using SGR, determined the optimum carbohydrate inclusion for dusky kob to be 16.72%. The general structure of the liver was similar between all fish fed the dietary treatments and all fish liver samples displayed a certain degree of lipid vacuolization of the hepatocytes. Evidence of starvation was observed in the livers of fish fed diet 24.6C. However, no differences in the amount of liver glycogen were observed. Gut bacterial composition did not differ among the different diets or between the different sections of the gut. Differences were however observed in the diversity of the bacterial community structure at the start (when they were fed commercial trout feed) and end of the experimental period (after being fed a fishmeal-maize diet). The rate at which glucose was cleared from the blood differed significantly between diets. The low carbohydrate diets (i.e. diet 4.1C and 8.2C) showed a steady rate of glucose removal from the blood over a 48 h experimental period. Sharp increases in blood glucose concentration were observed in diet 16.4C and diet 24.6C, with highest glucose concentrations of 7.18 ± 1.81 and 8.05 ± 2.35 mmol/l respectively, observed 24 h after feeding. The blood glucose concentration of the fish fed diet 16.4C however returned to resting glucose concentration after 48 h, while that of the fish fed diet 24.6C did not. The results demonstrated that the level of dietary carbohydrate inclusion has a significant effect on the growth and health of the fish. The optimum carbohydrate inclusion in dusky kob diets is indicated to be 16.7%, which gave the best growth rate and maximum protein sparing effect without adverse effects on fish health. These findings are important for diet formulation and producing ‘least-cost’ diets for dusky kob farming.
- Full Text:
- Date Issued: 2017
- Authors: Mabasa, Nyiko Charity
- Date: 2017
- Subjects: Carbohydrates in animal nutrition , Fishes -- Feeding and feeds , Argyrosomus japonicus , Argyrosomus , Argyrosomus japonicus -- Nutrition , Argyrosomus -- Nutrition , Argyrosomus japonicus -- Feeding and feeds , Argyrosomus -- Feeding and feeds
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/44895 , vital:25450
- Description: The dusky kob, Argyrosomus japonicus, is an emerging commercial marine aquaculture species in South Africa. Attributes such as market acceptance, fast growth rates, tolerance to sibling density, acceptance of pelleted feed and adaptability to intensive aquaculture conditions make it a good candidate. Feed, the largest running cost in most aquaculture operations, is a limiting factor in dusky kob production as its nutritional requirements are not well understood. The present project formed part of a research program to develop a locally produced, least cost and sustainable feed that will support the growth and health of dusky kob. The approximate protein and energy requirements for kob fed formulated feeds have been established in earlier research, but the ability of kob to utilize carbohydrates as an energy source has not previously been investigated. The aim of this study was to determine the efficiency of dusky kob to utilize graded levels of carbohydrate (pregelatinized maize starch - PGMS) in pelleted diets. The research objectives were to determine the effect of dietary carbohydrate levels on:i) growth and feed utilization; ii) post prandial blood glucose levels; and iii) general fish health, gut bacterial composition and liver glycogen content of juvenile dusky kob. Hatchery reared juvenile dusky kob (5 g) were acclimatized in a recirculating experimental aquaculture system at the Department of Ichthyology and Fisheries Science at Rhodes University. They were fed trout crumble starter feed for three weeks before the start of the feeding experiment. Five diets containing 4.1, 8.2, 16.4 and 24.6% carbohydrate, hereafter referred to as 4.1C, 8.2C, 16.4C and 24.6C respectively, were formulated with pregelatinized maize starch as a carbohydrate source and fishmeal as the main protein source. Fish were fed these diets twice daily at 3.85% body weight per day for three months. Specific growth rates and feed conversion rations differed significantly between the four dietary treatments. Growth rate increased with increasing carbohydrate up to 16.4%, after which a significant decline was observed. Diet 16.4C produced the highest specific growth rate (SGR) of 1.84 ± 0.05% body weight/day, and the lowest feed conversion ratio of 1.28 ± 0.08. While the lowest specific growth rate and highest feed conversion ratio of 0.79 ± 0.05 and 2.14 ± 0.13 respectively, were found for diet 24.6C. A third order polynomial regression, using SGR, determined the optimum carbohydrate inclusion for dusky kob to be 16.72%. The general structure of the liver was similar between all fish fed the dietary treatments and all fish liver samples displayed a certain degree of lipid vacuolization of the hepatocytes. Evidence of starvation was observed in the livers of fish fed diet 24.6C. However, no differences in the amount of liver glycogen were observed. Gut bacterial composition did not differ among the different diets or between the different sections of the gut. Differences were however observed in the diversity of the bacterial community structure at the start (when they were fed commercial trout feed) and end of the experimental period (after being fed a fishmeal-maize diet). The rate at which glucose was cleared from the blood differed significantly between diets. The low carbohydrate diets (i.e. diet 4.1C and 8.2C) showed a steady rate of glucose removal from the blood over a 48 h experimental period. Sharp increases in blood glucose concentration were observed in diet 16.4C and diet 24.6C, with highest glucose concentrations of 7.18 ± 1.81 and 8.05 ± 2.35 mmol/l respectively, observed 24 h after feeding. The blood glucose concentration of the fish fed diet 16.4C however returned to resting glucose concentration after 48 h, while that of the fish fed diet 24.6C did not. The results demonstrated that the level of dietary carbohydrate inclusion has a significant effect on the growth and health of the fish. The optimum carbohydrate inclusion in dusky kob diets is indicated to be 16.7%, which gave the best growth rate and maximum protein sparing effect without adverse effects on fish health. These findings are important for diet formulation and producing ‘least-cost’ diets for dusky kob farming.
- Full Text:
- Date Issued: 2017
Effect of feeding regimen, temperature and stocking density on growth and survival of juvenile clownfish (Amphiprion percula)
- Authors: Johnston, Gavin
- Date: 2001
- Subjects: Anemonefishes , Fishes -- Feeding and feeds , Fishes -- Growth
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5274 , http://hdl.handle.net/10962/d1005118 , Anemonefishes , Fishes -- Feeding and feeds , Fishes -- Growth
- Description: In aquaculture, a thorough knowledge of the specific environmental requirements of a species is needed in order to maximize growth rate and survival. There is a paucity of data regarding the fundamental environmental requirements for the ongrowing phase of clownfish juveniles. This prompted the design of three experiments to determine the best feeding regimen, temperature and stocking density that maximize growth and survival of Amphiprion percula. Ration size and feeding frequency are important factors for optimizing fish growth during the juvenile grow-out phase. A factorial growth trial was conducted to determine the effect of feeding frequency and ration size on the growth of juvenile clownfish (Amphiprion percula).Three feeding frequencies (1, 2 and 3 times daily) and six rations (2,4,6,8, 10 and 12 % body weight per day (BW.day⁻¹)) were used to test the growth response over a twelve week period. Non-linear regression analysis on the effect of ration, independent of feeding frequency, on growth resulted in a significant (n = 36; r² = 68.7) parabolic model: In y = -0.0302x² + 0.5159x + -4.4377. Maximum growth corresponded to a ration of 8.5% BW.day⁻¹. Survival as a function of ration was significantly lower at 2% BW.day⁻¹. Data were further examined with Analysis of CoVariance (ANCOVA) to determine the effect of ration on growth at each feeding frequency. The combination revealed a maximum growth rate when the fish were fed a ration of 10% BW.day⁻¹ divided into two equal meals. The required ration per meal to maintain maximum growth was also found to decrease as feeding frequency increased. The determination of the best temperature for growth is of great importance due to the direct relationship between fish metabolism and temperature. The thermal preferendum of A. percula has already been estimated at 26 ± 0.7°C but it is not known whether this closely approximates the temperature for maximum growth. Sixteen tanks were set to different temperatures ranging between 21.5 and 30.2 °C. Ten juvenile A. percula were placed in each tank and growth was measured fortnightly over the course of the 10 week experiment. Non-linear regression analysis resulted in significant models for fish length (y = -0.0005x² + 0.00267x - 0.0338; r² = 56.7, n = 11) and weight (y= -0.00016x² + 0.0084x - 0.1073; r² = 61.6, n = 11). These models predict that maximum growth would be at 27.7 and 27.1 °C for length and weight, respectively. Temperature, over the range tested, had no apparent effect on survival. There was no significant difference between the temperatures for maximum growth and the preferred temperature. The effect of stocking densities ranging from 0.2 fish.L¹ to 4.0 fish.L⁻¹ on growth were used in the third experiment. The fish were fed to satiation twice daily and growth was measured fortnightly throughout the 8 week experiment. No effects on growth, survival or coefficient of variation were found within the range of stocking densities tested.
- Full Text:
- Date Issued: 2001
- Authors: Johnston, Gavin
- Date: 2001
- Subjects: Anemonefishes , Fishes -- Feeding and feeds , Fishes -- Growth
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5274 , http://hdl.handle.net/10962/d1005118 , Anemonefishes , Fishes -- Feeding and feeds , Fishes -- Growth
- Description: In aquaculture, a thorough knowledge of the specific environmental requirements of a species is needed in order to maximize growth rate and survival. There is a paucity of data regarding the fundamental environmental requirements for the ongrowing phase of clownfish juveniles. This prompted the design of three experiments to determine the best feeding regimen, temperature and stocking density that maximize growth and survival of Amphiprion percula. Ration size and feeding frequency are important factors for optimizing fish growth during the juvenile grow-out phase. A factorial growth trial was conducted to determine the effect of feeding frequency and ration size on the growth of juvenile clownfish (Amphiprion percula).Three feeding frequencies (1, 2 and 3 times daily) and six rations (2,4,6,8, 10 and 12 % body weight per day (BW.day⁻¹)) were used to test the growth response over a twelve week period. Non-linear regression analysis on the effect of ration, independent of feeding frequency, on growth resulted in a significant (n = 36; r² = 68.7) parabolic model: In y = -0.0302x² + 0.5159x + -4.4377. Maximum growth corresponded to a ration of 8.5% BW.day⁻¹. Survival as a function of ration was significantly lower at 2% BW.day⁻¹. Data were further examined with Analysis of CoVariance (ANCOVA) to determine the effect of ration on growth at each feeding frequency. The combination revealed a maximum growth rate when the fish were fed a ration of 10% BW.day⁻¹ divided into two equal meals. The required ration per meal to maintain maximum growth was also found to decrease as feeding frequency increased. The determination of the best temperature for growth is of great importance due to the direct relationship between fish metabolism and temperature. The thermal preferendum of A. percula has already been estimated at 26 ± 0.7°C but it is not known whether this closely approximates the temperature for maximum growth. Sixteen tanks were set to different temperatures ranging between 21.5 and 30.2 °C. Ten juvenile A. percula were placed in each tank and growth was measured fortnightly over the course of the 10 week experiment. Non-linear regression analysis resulted in significant models for fish length (y = -0.0005x² + 0.00267x - 0.0338; r² = 56.7, n = 11) and weight (y= -0.00016x² + 0.0084x - 0.1073; r² = 61.6, n = 11). These models predict that maximum growth would be at 27.7 and 27.1 °C for length and weight, respectively. Temperature, over the range tested, had no apparent effect on survival. There was no significant difference between the temperatures for maximum growth and the preferred temperature. The effect of stocking densities ranging from 0.2 fish.L¹ to 4.0 fish.L⁻¹ on growth were used in the third experiment. The fish were fed to satiation twice daily and growth was measured fortnightly throughout the 8 week experiment. No effects on growth, survival or coefficient of variation were found within the range of stocking densities tested.
- Full Text:
- Date Issued: 2001
The biotechnology of effluent-grown Spirulina, and application in aquaculture nutrition
- Authors: Maart, Brenton Ashley
- Date: 1993
- Subjects: Aquaculture , Spirulina , Algae -- Biotechnology , Fishes -- Feeding and feeds
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4050 , http://hdl.handle.net/10962/d1004111 , Aquaculture , Spirulina , Algae -- Biotechnology , Fishes -- Feeding and feeds
- Description: The biotechnology of production and utilisation of the cyanobacterium Spirulina has been well documented. Research has centred mainly on application in human and animal nutrition, and has been motivated by the high protein, vitamin, fatty acid and growth factor contents. The main obstacle in realising the full potential of this feed source has been the high production costs associated with its mass culture in defined media. The observation of blooms of Spirulina in tannery effluent evaporation ponds in Wellington, South Africa, prompted this investigation into the harvesting, and nutritional and toxicological evaluation of this potentially low-cost production system, with the ultimate aim of using the product in aquaculture rations. An investigation of the chemical gradient along the evaporation cascade showed a positive correlation between the prevailing chemical conditions and the dominant species populations. A standing crop of 9.5 tonnes/ha of Spirulina was found to be present in the latter alkaline ponds, characterised by relatively lower organic and sulphur contents. Initial harvesting of the biomass was achieved by the design, construction and implementation of a small-scale screen harvest, which yielded a 25 kg (dry weight) crop. A scale-up model was then designed, and implemented in a technical scale harvest, yielding a crop of 250 kg (dry weight). Both these harvests utilised the bloom of surface-autoflocculated biomass. Concentrated cell slurries were sun-dried on muslin beds, and milled to a coarse powder. An evaluation of the harvest revealed a chemical content similar to other published reports of defined media cultures, with the exception of the protein and amino acid contents. The observed lower levels of the latter two are almost certainly due to the sun-drying method employed, known to reduce the protein content due to thermal denaturation. Legislation demands the strict toxicological evaluation of new protein sources, and because of the effluent-nature of the growth medium of this source of Spirulina, its viability lies only in the application as an animal feed or supplement. A range of toxicological tests were chosen that were targeted to elucidate the possible toxicological constraints of this effluentgrown source of protein in animal nutrition. The nucleic acid and pesticide contents of the harvested biomass were within the prescribed safety ranges. Atomic absorption showed minimal accumulation of minerals and heavy metals from the effluent. A bioassay with the brine shrimp Anemia salina showed that the biomass contained no toxicologically active water-soluble components. A short term feeding trial with new-born chicks showed that supplementation with Spirulina had no effect on the growth rates and feed conversion ratios of the different feeding groups. Pathological analyses showed that the liver was the only target organ to elicit a change in response to supplementation of the diets with Spirulina. A general decrease in liver weight was noted, with Cu, Ca, Fe and Zn being significantly accumulated. A histopathological examination however, showed no cellular and functional aberration from the control animals. The toxicological analyses gave the preliminary safe go-ahead for the evaluation of effluent-grown Spirulina in aquaculture nutrition. The South African abalone Haliotis midae, and the rainbow trout Oncorhynchus mykiss were chosen as representative species of edible cultured organisms. The technology for the culture of the perlemoen abalone is being established in South Africa, with the main area of research being the development of an artificial diet for high density culture. A 40 day growth trial demonstrated that lower concentrations of Spirulina supplemented to an agar-based fishmeal diet resulted in growth rates and feed conversion ratios similar to the control fishmeal and purified-casein diets, and thus has application potential in the nutrition of this high-cost marine delicacy. The aquaculture technology of freshwater rainbow trout is already well established. An eight week feeding trial with various concentrations of Spirulina showed that this effluent-grown protein source can partially replace fishmeal in semi-purified diets. Fish fed Spirulina did not exhibit decisive manifestations of toxicity, as determined in a histopathological study. In addition, Spirulina supplementation resulted in enhanced colouration of the skin and flesh, which may have implications in the aesthetic marketing of this sought-after table fish. The primary aim of this preliminary investigation thus concerned the determination of the biotechnological potential of this effluent-source of Spirulina. A technology transfer from the economically unfeasible defined-media culture was implemented. This project is ultimately aimed as a contribution towards the treatment of tannery wastewater, by the removal of contaminants from the effluent in the form of organic biomass.
- Full Text:
- Date Issued: 1993
- Authors: Maart, Brenton Ashley
- Date: 1993
- Subjects: Aquaculture , Spirulina , Algae -- Biotechnology , Fishes -- Feeding and feeds
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4050 , http://hdl.handle.net/10962/d1004111 , Aquaculture , Spirulina , Algae -- Biotechnology , Fishes -- Feeding and feeds
- Description: The biotechnology of production and utilisation of the cyanobacterium Spirulina has been well documented. Research has centred mainly on application in human and animal nutrition, and has been motivated by the high protein, vitamin, fatty acid and growth factor contents. The main obstacle in realising the full potential of this feed source has been the high production costs associated with its mass culture in defined media. The observation of blooms of Spirulina in tannery effluent evaporation ponds in Wellington, South Africa, prompted this investigation into the harvesting, and nutritional and toxicological evaluation of this potentially low-cost production system, with the ultimate aim of using the product in aquaculture rations. An investigation of the chemical gradient along the evaporation cascade showed a positive correlation between the prevailing chemical conditions and the dominant species populations. A standing crop of 9.5 tonnes/ha of Spirulina was found to be present in the latter alkaline ponds, characterised by relatively lower organic and sulphur contents. Initial harvesting of the biomass was achieved by the design, construction and implementation of a small-scale screen harvest, which yielded a 25 kg (dry weight) crop. A scale-up model was then designed, and implemented in a technical scale harvest, yielding a crop of 250 kg (dry weight). Both these harvests utilised the bloom of surface-autoflocculated biomass. Concentrated cell slurries were sun-dried on muslin beds, and milled to a coarse powder. An evaluation of the harvest revealed a chemical content similar to other published reports of defined media cultures, with the exception of the protein and amino acid contents. The observed lower levels of the latter two are almost certainly due to the sun-drying method employed, known to reduce the protein content due to thermal denaturation. Legislation demands the strict toxicological evaluation of new protein sources, and because of the effluent-nature of the growth medium of this source of Spirulina, its viability lies only in the application as an animal feed or supplement. A range of toxicological tests were chosen that were targeted to elucidate the possible toxicological constraints of this effluentgrown source of protein in animal nutrition. The nucleic acid and pesticide contents of the harvested biomass were within the prescribed safety ranges. Atomic absorption showed minimal accumulation of minerals and heavy metals from the effluent. A bioassay with the brine shrimp Anemia salina showed that the biomass contained no toxicologically active water-soluble components. A short term feeding trial with new-born chicks showed that supplementation with Spirulina had no effect on the growth rates and feed conversion ratios of the different feeding groups. Pathological analyses showed that the liver was the only target organ to elicit a change in response to supplementation of the diets with Spirulina. A general decrease in liver weight was noted, with Cu, Ca, Fe and Zn being significantly accumulated. A histopathological examination however, showed no cellular and functional aberration from the control animals. The toxicological analyses gave the preliminary safe go-ahead for the evaluation of effluent-grown Spirulina in aquaculture nutrition. The South African abalone Haliotis midae, and the rainbow trout Oncorhynchus mykiss were chosen as representative species of edible cultured organisms. The technology for the culture of the perlemoen abalone is being established in South Africa, with the main area of research being the development of an artificial diet for high density culture. A 40 day growth trial demonstrated that lower concentrations of Spirulina supplemented to an agar-based fishmeal diet resulted in growth rates and feed conversion ratios similar to the control fishmeal and purified-casein diets, and thus has application potential in the nutrition of this high-cost marine delicacy. The aquaculture technology of freshwater rainbow trout is already well established. An eight week feeding trial with various concentrations of Spirulina showed that this effluent-grown protein source can partially replace fishmeal in semi-purified diets. Fish fed Spirulina did not exhibit decisive manifestations of toxicity, as determined in a histopathological study. In addition, Spirulina supplementation resulted in enhanced colouration of the skin and flesh, which may have implications in the aesthetic marketing of this sought-after table fish. The primary aim of this preliminary investigation thus concerned the determination of the biotechnological potential of this effluent-source of Spirulina. A technology transfer from the economically unfeasible defined-media culture was implemented. This project is ultimately aimed as a contribution towards the treatment of tannery wastewater, by the removal of contaminants from the effluent in the form of organic biomass.
- Full Text:
- Date Issued: 1993
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