Assessment of the flocculating efficiency of bioflocculant produced by bacillus sp. Aemreg4 isolated from Tyhume river, Eastern Cape, South Africa
- Authors: Ntsangani, Nozipho
- Date: 2016
- Subjects: Flocculants
- Language: English
- Type: Thesis , Masters , MSc (Biochemistry)
- Identifier: vital:11357 , http://hdl.handle.net/10353/d1021324
- Description: Bioflocculants are flocculating substances produced by microorganisms during growth and have recently received considerable attention from researchers; due to their biodegradability, non-toxicity and lack of secondary pollution from degradation intermediates. This study evaluated the efficiency of bioflocculant produced by Bacillus sp. AEMREG4 isolated from Tyhume River. The bacterial identification was through 16S rDNA sequencing; nucleotide sequences were deposited in GenBank as Bacillus sp. AEMREG4 with an Accession number KP406729. The optimum culture conditions for bioflocculant production were an inoculum size of 4% (v/v) and starch as well as yeast extract as sole carbon and nitrogen sources respectively. The addition of CaCl2 enhanced the flocculating activity, at a wide range of pH 4-10 and the highest flocculating activity was reached at an initial pH 8 (80%). A bioflocculant yield of 0.78 g was recovered from 1 L of culture broth. The optimum flocculating activity of 78% was reached at the lowest bioflocculant dosage of 0.1 mg/ml and the presence of divalent cations (Ca2+, Mn2+ and Mg2+) as well as a trivalent cation (Al3+) enhanced flocculating activity. The purified bioflocculant retained more than 70% flocculating activity when subjected to heating at 100 °C for 1 h and maximum flocculating activity of 83% was achieved at both acidic and basic pH values of 3 and 10 respectively. Chemical analysis showed that the bioflocculant is predominantly polysaccharide. The Fourier transform infrared (FTIR) spectrum revealed the presence of carboxyl, hydroxyl and methoxyl groups as the functional moieties and the scanning electron microscopy (SEM) imaging of the purified bioflocculant showed its morphological structure as rod-shaped which contributes to its high flocculating efficiency. The high flocculation activity displayed by this bioflocculant indicates its potential suitability for industrial application.Keywords: Bioflocculant, Bacillus sp. AEMREG4, flocculating activity.
- Full Text:
- Date Issued: 2016
- Authors: Ntsangani, Nozipho
- Date: 2016
- Subjects: Flocculants
- Language: English
- Type: Thesis , Masters , MSc (Biochemistry)
- Identifier: vital:11357 , http://hdl.handle.net/10353/d1021324
- Description: Bioflocculants are flocculating substances produced by microorganisms during growth and have recently received considerable attention from researchers; due to their biodegradability, non-toxicity and lack of secondary pollution from degradation intermediates. This study evaluated the efficiency of bioflocculant produced by Bacillus sp. AEMREG4 isolated from Tyhume River. The bacterial identification was through 16S rDNA sequencing; nucleotide sequences were deposited in GenBank as Bacillus sp. AEMREG4 with an Accession number KP406729. The optimum culture conditions for bioflocculant production were an inoculum size of 4% (v/v) and starch as well as yeast extract as sole carbon and nitrogen sources respectively. The addition of CaCl2 enhanced the flocculating activity, at a wide range of pH 4-10 and the highest flocculating activity was reached at an initial pH 8 (80%). A bioflocculant yield of 0.78 g was recovered from 1 L of culture broth. The optimum flocculating activity of 78% was reached at the lowest bioflocculant dosage of 0.1 mg/ml and the presence of divalent cations (Ca2+, Mn2+ and Mg2+) as well as a trivalent cation (Al3+) enhanced flocculating activity. The purified bioflocculant retained more than 70% flocculating activity when subjected to heating at 100 °C for 1 h and maximum flocculating activity of 83% was achieved at both acidic and basic pH values of 3 and 10 respectively. Chemical analysis showed that the bioflocculant is predominantly polysaccharide. The Fourier transform infrared (FTIR) spectrum revealed the presence of carboxyl, hydroxyl and methoxyl groups as the functional moieties and the scanning electron microscopy (SEM) imaging of the purified bioflocculant showed its morphological structure as rod-shaped which contributes to its high flocculating efficiency. The high flocculation activity displayed by this bioflocculant indicates its potential suitability for industrial application.Keywords: Bioflocculant, Bacillus sp. AEMREG4, flocculating activity.
- Full Text:
- Date Issued: 2016
Production and characterization of a bioflocculant from a consortium of bacteria belonging to the halomonas and micrococcus genera.
- Okaiyeto, Kunle (https://orcid.org/0000-0002-7211-714X)
- Authors: Okaiyeto, Kunle (https://orcid.org/0000-0002-7211-714X)
- Date: 2013
- Subjects: Flocculants , Bacteria
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/26119 , vital:64917
- Description: The physicochemical properties of two bioflocculant producing bacteria; Halomonas sp. Okoh and Micrococcus sp. Leo were investigated. The optimum culture conditions for the individual species were determined. All the growth conditions examined for the individual bacteria were similar. Glucose and ammonium sulphate as sole carbon and nitrogen sources respectively resulted in optimum production of bioflocculant. The flocculating activity of the bioflocculants was stimulated when Al3+ was used as the coagulating aid under acidic medium. The information obtained from individual strains was used to produce a bioflocculant from the consortium of the two bacteria. After purification, the bioflocculant yields from 1L fermentation broths were 1.213 g from Halomonas sp. Okoh, 0.738 g from Micrococcus sp. Leo and 3.51 g from the consortium. The chemical analyses of the purified bioflocculants showed that they were glycoproteins. The thermostability property of the bioflocculants was investigated between 50-100oC and the results revealed that they are heat-stable. Fourier transform infrared revealed the presence of hydroxyl, carboxyl and amino groups in the bioflocculant molecules. Scaning electron microscope (SEM) images showed the structure of each bioflocculant(s) and kaolin clay before and after flocculation. From the results obtained, the idea of using the two strains in consortium for bioflocculant production resulted in an improvement in terms of flocculating activity and yield. The bioflocculants appears to have promise as an alternative to chemical flocculants used in various industrial processes such as wastewater treatment and drinking water purification. , Thesis (MA) -- Faculty Science and Agriculture, 2013
- Full Text:
- Date Issued: 2013
- Authors: Okaiyeto, Kunle (https://orcid.org/0000-0002-7211-714X)
- Date: 2013
- Subjects: Flocculants , Bacteria
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/26119 , vital:64917
- Description: The physicochemical properties of two bioflocculant producing bacteria; Halomonas sp. Okoh and Micrococcus sp. Leo were investigated. The optimum culture conditions for the individual species were determined. All the growth conditions examined for the individual bacteria were similar. Glucose and ammonium sulphate as sole carbon and nitrogen sources respectively resulted in optimum production of bioflocculant. The flocculating activity of the bioflocculants was stimulated when Al3+ was used as the coagulating aid under acidic medium. The information obtained from individual strains was used to produce a bioflocculant from the consortium of the two bacteria. After purification, the bioflocculant yields from 1L fermentation broths were 1.213 g from Halomonas sp. Okoh, 0.738 g from Micrococcus sp. Leo and 3.51 g from the consortium. The chemical analyses of the purified bioflocculants showed that they were glycoproteins. The thermostability property of the bioflocculants was investigated between 50-100oC and the results revealed that they are heat-stable. Fourier transform infrared revealed the presence of hydroxyl, carboxyl and amino groups in the bioflocculant molecules. Scaning electron microscope (SEM) images showed the structure of each bioflocculant(s) and kaolin clay before and after flocculation. From the results obtained, the idea of using the two strains in consortium for bioflocculant production resulted in an improvement in terms of flocculating activity and yield. The bioflocculants appears to have promise as an alternative to chemical flocculants used in various industrial processes such as wastewater treatment and drinking water purification. , Thesis (MA) -- Faculty Science and Agriculture, 2013
- Full Text:
- Date Issued: 2013
Assessment of bioflocculant production by some marine bacteria isolated from the bottom sediment of Algoa Bay
- Authors: Cosa, Sekelwa
- Date: 2010
- Subjects: Flocculants , Bacteria -- South Africa -- Algoa Bay
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11244 , http://hdl.handle.net/10353/404 , Flocculants , Bacteria -- South Africa -- Algoa Bay
- Description: Several problems concerning the use of conventional synthetic flocculants has necessitated the need for alternative cost effective, safe and efficient bioflocculants from microorganisms inhabiting many environments, particularly those from unusual environments. Hence, this study assessed bioflocculant production by three marine bacteria isolated from the bottom sediment of Algoa Bay in the Eastern Cape Province of South Africa. Analysis of the 16S rDNA sequences led to their identification as Halobacillus sp. Mvuyo, Virgibacillus sp. Rob and Oceanobacillus sp. Pinky. Several factors affecting the production and activity of the bioflocculant(s) were studied. Halobacillus sp. Mvuyo produced bioflocculant optimally with glucose (76%) and ammonium chloride (93%) as sole carbon and nitrogen sources, respectively and at neutral pH and in the presence of Ca2+. On the other hand, Virgibacillus sp. Rob preferred glucose (70.4 %) and iron sulphate (74%) as carbon and nitrogen source respectively; an alkaline pH (12.0) and Fe2+. Oceanobacillus sp. Pinky produced bioflocculant optimally when sucrose (80%) and peptone (72.4 %) were used as carbon and nitrogen source respectively, at neutral pH, and in the presence of Ca2+ cation. The chemical analyses of the partially purified bioflocculants revealed that the bioflocculants produced by Halobacillus sp. Mvuyo and Oceanobacillus sp. Pinky were glycoproteins, while that produced by Virgibacillus sp. Rob was a polysaccharide. We thus conclude that Halobacillus sp. Mvuyo, Virgibacillus sp. Rob and Oceanobacillus sp. Pinky hold promise as producers of new and efficient bioflocculant(s). We recommended development of process conditions for large scale production of the bioflocculants followed by their detailed characterization, as well as pilot scale assessment of the applicability of the purified bioflocculant in water/wastewater treatment and other industrial uses
- Full Text:
- Date Issued: 2010
- Authors: Cosa, Sekelwa
- Date: 2010
- Subjects: Flocculants , Bacteria -- South Africa -- Algoa Bay
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11244 , http://hdl.handle.net/10353/404 , Flocculants , Bacteria -- South Africa -- Algoa Bay
- Description: Several problems concerning the use of conventional synthetic flocculants has necessitated the need for alternative cost effective, safe and efficient bioflocculants from microorganisms inhabiting many environments, particularly those from unusual environments. Hence, this study assessed bioflocculant production by three marine bacteria isolated from the bottom sediment of Algoa Bay in the Eastern Cape Province of South Africa. Analysis of the 16S rDNA sequences led to their identification as Halobacillus sp. Mvuyo, Virgibacillus sp. Rob and Oceanobacillus sp. Pinky. Several factors affecting the production and activity of the bioflocculant(s) were studied. Halobacillus sp. Mvuyo produced bioflocculant optimally with glucose (76%) and ammonium chloride (93%) as sole carbon and nitrogen sources, respectively and at neutral pH and in the presence of Ca2+. On the other hand, Virgibacillus sp. Rob preferred glucose (70.4 %) and iron sulphate (74%) as carbon and nitrogen source respectively; an alkaline pH (12.0) and Fe2+. Oceanobacillus sp. Pinky produced bioflocculant optimally when sucrose (80%) and peptone (72.4 %) were used as carbon and nitrogen source respectively, at neutral pH, and in the presence of Ca2+ cation. The chemical analyses of the partially purified bioflocculants revealed that the bioflocculants produced by Halobacillus sp. Mvuyo and Oceanobacillus sp. Pinky were glycoproteins, while that produced by Virgibacillus sp. Rob was a polysaccharide. We thus conclude that Halobacillus sp. Mvuyo, Virgibacillus sp. Rob and Oceanobacillus sp. Pinky hold promise as producers of new and efficient bioflocculant(s). We recommended development of process conditions for large scale production of the bioflocculants followed by their detailed characterization, as well as pilot scale assessment of the applicability of the purified bioflocculant in water/wastewater treatment and other industrial uses
- Full Text:
- Date Issued: 2010
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