Characterising termite mound spatial patterns in the Eastern Cape Karoo : applying drone remote sensing,GIS and spatial statistics
- Mngcele, Lizalise Sive Nqaba
- Authors: Mngcele, Lizalise Sive Nqaba
- Date: 2022-12
- Subjects: Drone aircraft in remote sensing – Eastern Cape Karoo , Termites , Geographic information Systems – South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59555 , vital:62169
- Description: The Eastern Cape Karoo in South Africa has been earmarked for potential Shale Gas development, which has necessitated the understanding of existing ecosystems to be quantiĄed pre-development, in order to have a baseline against which the exploration can be monitored. Termite mounds as baseline mechanisms, are known to be sensitive to ecosystem disturbance and because of their abundance in the exploration zone, have been studied as indicator species. They are both a surface and subsurface phenomena which makes them an ideal baseline monitoring mechanism. Termite mound height, basal circumference and geospatial data was collected against natural and anthropogenic factors: elevation, vegetation, water, soil, geology, human settlements and roads. Mound distributions were observed across four study sites, and seven plots, using a DJI Phantom 4 Pro drone, an aerial and ground survey. Observed mound data on the drone and aerial survey was compared to that of the ground survey. Overall, the drone survey outperformed the aerial survey in recording accurate termite mound data. This was largely attributed to the scale of the study which gave the drone a competitive advantage. It allowed for drone data to be collected at 40 m altitude with an image resolution of 2-6 cm/pixel on each plot. In addition, drone detection accuracy was improved through the ability to generate digital surface models (DSMs) through point clouds and overlaying them with orthomosaics. Considering observed mound spatial point patterns, both the drone and aerial survey were more than 50% percent consistent with the ground survey, although the drone survey detected 28.57% more accurate mound spatial point patterns than the aerial survey. , Thesis (MSc) -- Faculty of Science, School of Environmental Sciences, 2022
- Full Text:
- Date Issued: 2022-12
- Authors: Mngcele, Lizalise Sive Nqaba
- Date: 2022-12
- Subjects: Drone aircraft in remote sensing – Eastern Cape Karoo , Termites , Geographic information Systems – South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59555 , vital:62169
- Description: The Eastern Cape Karoo in South Africa has been earmarked for potential Shale Gas development, which has necessitated the understanding of existing ecosystems to be quantiĄed pre-development, in order to have a baseline against which the exploration can be monitored. Termite mounds as baseline mechanisms, are known to be sensitive to ecosystem disturbance and because of their abundance in the exploration zone, have been studied as indicator species. They are both a surface and subsurface phenomena which makes them an ideal baseline monitoring mechanism. Termite mound height, basal circumference and geospatial data was collected against natural and anthropogenic factors: elevation, vegetation, water, soil, geology, human settlements and roads. Mound distributions were observed across four study sites, and seven plots, using a DJI Phantom 4 Pro drone, an aerial and ground survey. Observed mound data on the drone and aerial survey was compared to that of the ground survey. Overall, the drone survey outperformed the aerial survey in recording accurate termite mound data. This was largely attributed to the scale of the study which gave the drone a competitive advantage. It allowed for drone data to be collected at 40 m altitude with an image resolution of 2-6 cm/pixel on each plot. In addition, drone detection accuracy was improved through the ability to generate digital surface models (DSMs) through point clouds and overlaying them with orthomosaics. Considering observed mound spatial point patterns, both the drone and aerial survey were more than 50% percent consistent with the ground survey, although the drone survey detected 28.57% more accurate mound spatial point patterns than the aerial survey. , Thesis (MSc) -- Faculty of Science, School of Environmental Sciences, 2022
- Full Text:
- Date Issued: 2022-12
Characterization of termite Trinervitermes trinervoides metagenome-derived glycoside hydrolases, the formulation of synergistic core enzyme sets for effective sweet sorghum and corncob saccharification, and their potential industrial applications
- Authors: Mafa, Mpho Stephen
- Date: 2019
- Subjects: Termites , Metagenomics , Glucosides , Hydrolases , Enzymes , Feedstock
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/72414 , vital:30044 , DOI https://doi.org/10.21504/10962/72414
- Description: The current study investigated the biochemical properties of endo-glucanase (GH5E), exo-glucanase (GH5D), xylanase (GH5H) and endo-glucanase/xylanase (GH45), derived from the hindgut bacterial symbionts of a termite (Trinervitermes trinervoides) for their potential role in the biotechnology industry. All these enzymes, except GH5D, exhibited activities on cellulosic and xylan-rich polymeric substrates, which only displayed activity on p-nitrophenyl cellobioside. GH5D, GH5E, GH5H and GH45 enzymes retained more than 80% of their activities at pH 5.5 and also retained more than 80% of their activities at 40ºC. Furthermore, these enzymes were thermostable at 37ºC for 72 hours. GH5E, GH5H and GH45 were generally stable over a range of metal-ion. The kinetic parameters for GH5E were 5.68 mg/ml (KM) and 34.36 U/mg protein (Vmax). GH5D activity did not follow classical Michaelis-Menten kinetics, suggesting product inhibition. GH5H displayed KM values of 5.53, 95.03 and 2.10 mg/ml and Vmax values of 112.36, 144.45 and 180.32 U/mg protein on beechwood xylan, CMC, and xyloglucan, respectively. GH45 displayed a KM of 6.94 mg/ml and a Vmax of 12.30 U/mg protein on CMC. GH5D [cellobiohydrolase (CBH)] and a commercial CBHII (GH6) enzyme outperformed a commercial CBHI (GH7) enzyme when these enzymes hydrolysed β-glucan. GH5D and CBHII also displayed a higher degree of synergy on β-glucan but failed to show synergy on Avicel. We therefore concluded that GH5D and CBHII are β-glucan-specific cellobiohydrolases. The corncob (CC) and sweet sorghum bagasse (SSB) substrates were pretreated with lime, NaOH and NaClO2. Subsequent to pretreatment, these substrates were used to investigate if GH5D, GH5E, GH5H and GH45 could operate in synergy. Results revealed that out of 12 possible core enzyme sets constructed, only two (referred to as CES-E and CES-H) displayed higher activities on pretreated CC or SSB. Simultaneous synergy was generally the most effective mode of synergy during hydrolysis of alkaline pretreated SSB and CC samples by both CES-E and CES-H. Both core enzyme sets did not display synergy on oxidative pretreated substrates. These findings suggest that lime and NaOH are more effective pretreatments for CC and SSB substrates. We used PRotein Interactive MOdeling (PRIMO) software to demonstrate that GH5D protein structure is an (α/β)8 barrel with a tunnel-like active site. Enzymes with this type of protein structure are able to perform transglycosylation, a process in which GH5D produced methyl, ethyl and propyl cellobiosides. We concluded that the GH5D, GH5E, GH5H and GH45 enzymes possess novel biochemical properties and that they form synergy during the hydrolysis of complex substrates (SSB and CC). GH5D transglycosylation could be used to produce novel biodegradable chemicals with special properties (e.g. anti-microbial properties). In conclusion, our findings suggest that GH5D, GH5E, GH5H and GH45 can potentially be used to improve biorefinery processes. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2019
- Full Text:
- Date Issued: 2019
- Authors: Mafa, Mpho Stephen
- Date: 2019
- Subjects: Termites , Metagenomics , Glucosides , Hydrolases , Enzymes , Feedstock
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/72414 , vital:30044 , DOI https://doi.org/10.21504/10962/72414
- Description: The current study investigated the biochemical properties of endo-glucanase (GH5E), exo-glucanase (GH5D), xylanase (GH5H) and endo-glucanase/xylanase (GH45), derived from the hindgut bacterial symbionts of a termite (Trinervitermes trinervoides) for their potential role in the biotechnology industry. All these enzymes, except GH5D, exhibited activities on cellulosic and xylan-rich polymeric substrates, which only displayed activity on p-nitrophenyl cellobioside. GH5D, GH5E, GH5H and GH45 enzymes retained more than 80% of their activities at pH 5.5 and also retained more than 80% of their activities at 40ºC. Furthermore, these enzymes were thermostable at 37ºC for 72 hours. GH5E, GH5H and GH45 were generally stable over a range of metal-ion. The kinetic parameters for GH5E were 5.68 mg/ml (KM) and 34.36 U/mg protein (Vmax). GH5D activity did not follow classical Michaelis-Menten kinetics, suggesting product inhibition. GH5H displayed KM values of 5.53, 95.03 and 2.10 mg/ml and Vmax values of 112.36, 144.45 and 180.32 U/mg protein on beechwood xylan, CMC, and xyloglucan, respectively. GH45 displayed a KM of 6.94 mg/ml and a Vmax of 12.30 U/mg protein on CMC. GH5D [cellobiohydrolase (CBH)] and a commercial CBHII (GH6) enzyme outperformed a commercial CBHI (GH7) enzyme when these enzymes hydrolysed β-glucan. GH5D and CBHII also displayed a higher degree of synergy on β-glucan but failed to show synergy on Avicel. We therefore concluded that GH5D and CBHII are β-glucan-specific cellobiohydrolases. The corncob (CC) and sweet sorghum bagasse (SSB) substrates were pretreated with lime, NaOH and NaClO2. Subsequent to pretreatment, these substrates were used to investigate if GH5D, GH5E, GH5H and GH45 could operate in synergy. Results revealed that out of 12 possible core enzyme sets constructed, only two (referred to as CES-E and CES-H) displayed higher activities on pretreated CC or SSB. Simultaneous synergy was generally the most effective mode of synergy during hydrolysis of alkaline pretreated SSB and CC samples by both CES-E and CES-H. Both core enzyme sets did not display synergy on oxidative pretreated substrates. These findings suggest that lime and NaOH are more effective pretreatments for CC and SSB substrates. We used PRotein Interactive MOdeling (PRIMO) software to demonstrate that GH5D protein structure is an (α/β)8 barrel with a tunnel-like active site. Enzymes with this type of protein structure are able to perform transglycosylation, a process in which GH5D produced methyl, ethyl and propyl cellobiosides. We concluded that the GH5D, GH5E, GH5H and GH45 enzymes possess novel biochemical properties and that they form synergy during the hydrolysis of complex substrates (SSB and CC). GH5D transglycosylation could be used to produce novel biodegradable chemicals with special properties (e.g. anti-microbial properties). In conclusion, our findings suggest that GH5D, GH5E, GH5H and GH45 can potentially be used to improve biorefinery processes. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2019
- Full Text:
- Date Issued: 2019
- «
- ‹
- 1
- ›
- »