The effect of sewage effluent from De Beers marine diamond mining operations on the expression of cytochrome P450 (CYP1A) and vitellogenin (vtg)
- Authors: De Almeida, Louise
- Date: 2013-09-20
- Subjects: De Beers Consolidated Mines , Sewage disposal in rivers, lakes, etc. -- Namibia , Endocrine disrupting chemicals in water -- Namibia , Merluccius capensis -- Namibia , Merluccius -- Namibia , Water -- Pollution -- Namibia
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4096 , http://hdl.handle.net/10962/d1009440 , De Beers Consolidated Mines , Sewage disposal in rivers, lakes, etc. -- Namibia , Endocrine disrupting chemicals in water -- Namibia , Merluccius capensis -- Namibia , Merluccius -- Namibia , Water -- Pollution -- Namibia
- Description: Sewage effluents disposed into the marine environment from De Beers Marine Namibia diamond mining vessels have the potential to cause endocrine disruptive effects in marine organisms. Endocrine disruption refers to the alteration of the normal functioning of the endocrine system and various chemicals have the ability to mimic hormones, effecting endogenous hormone synthesis, transport, receptor interaction and intracellular signaling. The potential endocrine disruptive effects, caused by the release of different types of sewage effluents into the ocean, on fish species is a concern due to the commercial importance of fish species found in the mining area e.g. hake, sole, horse mackerel. Increased awareness of marine environmental degradation due to the presence of chemical contaminants has resulted in research being done on early warning systems, in the form of biomarkers. Cytochrome P450 monooxygenase 1A (CYP1A) and vitellogenin (vtg) are important proteins found in fish liver and blood, that have been used as biomarkers for the detection of pollutants in fish. CYP1A is a subfamily of the P450 superfamily of enzymes and catalyzes the oxidation, hydrolysis and reduction of exogenous and endogenous compounds (phase I reactions) and thus has the capacity to regulate the metabolism of several organic contaminants. CYP1A expression is altered by exposure to planar xenobiotic compounds e.g. polyaromatic hydrocarbons. Vtg is an important precursor for egg yolk proteins and plays a role in the growth and development of an oocyte. Expression of this protein is altered upon exposure to estrogenic compounds. The aim of this project was to isolate CYP1A from fish liver by differential centrifugation and optimize conditions for the CYP1A-mediated ethoxyresorufin-Odeethylase (EROD) assay and western blot analysis (to assess CYP1A expression). Another aim of this study was to evaluate the potential effects of biologically disruptive chemicals from sewage effluents, discharged into the marine environment, on the expression of CYP1A in two species of hake, Merluccius capensis and M. paradoxus (Cape hake). CYP1A in Cape hake is approximately a 60 kDa protein and the highest EROD activity was detected in the microsomal fraction after differential centrifugation. Optimal EROD assay conditions were observed at pH 7.5, a temperature of 25 °C, 10 μl of sample and a reaction time of 30 seconds. Enzyme stability assays indicated a drastic decrease in enzyme activity after 30 seconds. The EROD assay was not NADPH dependent but was limited by NADPH supply, with an increase of 300% in EROD activity being observed with the addition of 0.1 M exogenous NADPH. The addition of dicumarol (40 μM), a phase II enzyme inhibitor, showed a 232% increase in EROD activity. This is because dicumarol inhibited enzymes with the capacity to metabolize the product (resorufin) of the EROD reaction. With regard to western blot analysis, the optimal primary (rabbit antifish CYP1A peptide) and secondary (anti-mouse/rabbit antibody-horseradish peroxidase conjugate (POD)) antibody dilutions were determined to be 1:1000 and 1:5000, respectively. The comparison of CYP1A expression in Cape hake samples from De Beers Marine mining area and reference sites showed higher EROD activity (16.29 ± 0.91 pmol/min) in fish samples from the mining area in comparison to the reference site (10.42 ± 2.65 pmol/min). Western blot analysis was in agreement with the EROD assay results and a higher CYP1A expression was observed in fish from the mining sites. The increased CYP1A expression observed in fish from the mining area is not definitively an indication of a pollutant effect in the environment, as several environmental and biological factors (e.g. photoperiod and age) must also be considered before reaching this conclusion. Another aim of this study was to purify vtg from Cape hake blood samples. Cape hake vtg was purified from fish plasma by selective precipitation with MgCl2 and EDTA. Precipitated sample was subjected to anion exchange chromatography using fast protein liquid chromatography (FPLC). Vtg eluted as two broad peaks and had a molecular weight above 200 kDa. SDS-PAGE analysis also resolved smaller molecular weight proteins below 70 kDa, which were thought to be vitellogenin cleavage proteins, lipovitellin and phosphovitins. Western blot analysis was performed; however, it did not produce any conclusive results. The purification of vtg enables further studies in characterizing this protein and developing assay aimed at detecting estrogenic pollutants in the marine environment
- Full Text:
- Authors: De Almeida, Louise
- Date: 2013-09-20
- Subjects: De Beers Consolidated Mines , Sewage disposal in rivers, lakes, etc. -- Namibia , Endocrine disrupting chemicals in water -- Namibia , Merluccius capensis -- Namibia , Merluccius -- Namibia , Water -- Pollution -- Namibia
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4096 , http://hdl.handle.net/10962/d1009440 , De Beers Consolidated Mines , Sewage disposal in rivers, lakes, etc. -- Namibia , Endocrine disrupting chemicals in water -- Namibia , Merluccius capensis -- Namibia , Merluccius -- Namibia , Water -- Pollution -- Namibia
- Description: Sewage effluents disposed into the marine environment from De Beers Marine Namibia diamond mining vessels have the potential to cause endocrine disruptive effects in marine organisms. Endocrine disruption refers to the alteration of the normal functioning of the endocrine system and various chemicals have the ability to mimic hormones, effecting endogenous hormone synthesis, transport, receptor interaction and intracellular signaling. The potential endocrine disruptive effects, caused by the release of different types of sewage effluents into the ocean, on fish species is a concern due to the commercial importance of fish species found in the mining area e.g. hake, sole, horse mackerel. Increased awareness of marine environmental degradation due to the presence of chemical contaminants has resulted in research being done on early warning systems, in the form of biomarkers. Cytochrome P450 monooxygenase 1A (CYP1A) and vitellogenin (vtg) are important proteins found in fish liver and blood, that have been used as biomarkers for the detection of pollutants in fish. CYP1A is a subfamily of the P450 superfamily of enzymes and catalyzes the oxidation, hydrolysis and reduction of exogenous and endogenous compounds (phase I reactions) and thus has the capacity to regulate the metabolism of several organic contaminants. CYP1A expression is altered by exposure to planar xenobiotic compounds e.g. polyaromatic hydrocarbons. Vtg is an important precursor for egg yolk proteins and plays a role in the growth and development of an oocyte. Expression of this protein is altered upon exposure to estrogenic compounds. The aim of this project was to isolate CYP1A from fish liver by differential centrifugation and optimize conditions for the CYP1A-mediated ethoxyresorufin-Odeethylase (EROD) assay and western blot analysis (to assess CYP1A expression). Another aim of this study was to evaluate the potential effects of biologically disruptive chemicals from sewage effluents, discharged into the marine environment, on the expression of CYP1A in two species of hake, Merluccius capensis and M. paradoxus (Cape hake). CYP1A in Cape hake is approximately a 60 kDa protein and the highest EROD activity was detected in the microsomal fraction after differential centrifugation. Optimal EROD assay conditions were observed at pH 7.5, a temperature of 25 °C, 10 μl of sample and a reaction time of 30 seconds. Enzyme stability assays indicated a drastic decrease in enzyme activity after 30 seconds. The EROD assay was not NADPH dependent but was limited by NADPH supply, with an increase of 300% in EROD activity being observed with the addition of 0.1 M exogenous NADPH. The addition of dicumarol (40 μM), a phase II enzyme inhibitor, showed a 232% increase in EROD activity. This is because dicumarol inhibited enzymes with the capacity to metabolize the product (resorufin) of the EROD reaction. With regard to western blot analysis, the optimal primary (rabbit antifish CYP1A peptide) and secondary (anti-mouse/rabbit antibody-horseradish peroxidase conjugate (POD)) antibody dilutions were determined to be 1:1000 and 1:5000, respectively. The comparison of CYP1A expression in Cape hake samples from De Beers Marine mining area and reference sites showed higher EROD activity (16.29 ± 0.91 pmol/min) in fish samples from the mining area in comparison to the reference site (10.42 ± 2.65 pmol/min). Western blot analysis was in agreement with the EROD assay results and a higher CYP1A expression was observed in fish from the mining sites. The increased CYP1A expression observed in fish from the mining area is not definitively an indication of a pollutant effect in the environment, as several environmental and biological factors (e.g. photoperiod and age) must also be considered before reaching this conclusion. Another aim of this study was to purify vtg from Cape hake blood samples. Cape hake vtg was purified from fish plasma by selective precipitation with MgCl2 and EDTA. Precipitated sample was subjected to anion exchange chromatography using fast protein liquid chromatography (FPLC). Vtg eluted as two broad peaks and had a molecular weight above 200 kDa. SDS-PAGE analysis also resolved smaller molecular weight proteins below 70 kDa, which were thought to be vitellogenin cleavage proteins, lipovitellin and phosphovitins. Western blot analysis was performed; however, it did not produce any conclusive results. The purification of vtg enables further studies in characterizing this protein and developing assay aimed at detecting estrogenic pollutants in the marine environment
- Full Text:
A framework for successful SOA adoption in selected South African universities
- Authors: Jacobs, Carmen
- Date: 2013-07-26
- Subjects: System analysis -- Data processing , System design -- Data processing , Systems integration , Service-oriented architecture (Computer science) , Education, Higher -- Data processing
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:1153 , http://hdl.handle.net/10962/d1008366 , System analysis -- Data processing , System design -- Data processing , Systems integration , Service-oriented architecture (Computer science) , Education, Higher -- Data processing
- Description: The demand for systems i·ntegration has become more and more significant in higher education as institutions want systems that provide coherent information with data that is up to date and not redundant and can seamlessly support the end user experience. Institutions have become more reliant on information systems to support both administrative functions and those involved in teaching, learning and research, but because each department supports a diverse array of computing platforms and applications, it becomes very difficult to integrate these systems. SOA is classified as an innovative approach to integrating existing systems involving the use of independent services that can be accessed without knowledge of the underlying platform implementation. Unfortunately, the SOA initiative will not be success if it is not understood and used correctly by various applications and systems throughout the organisation. SOA introduces complexity and challenges in systems integration, acceptance, governance, data, development planning, security and external opportunities. If an organisation does not embrace or enable change in each of these areas, it is not ready for the adoption of SOA. This research investigates the systems integration challenge in selected South African universities and explores factors for SOA adoption. The framework for the adoption of SOA comprises seven factors, of which Systems Integration is the most significant and represents an efficient starting point for institutions considering SOA adoption. Acceptance, Governance, Data, Development Planning, Security and External Opportunities are other factors of SOA adoption that require careful and thorough consideration before an institution can successfully adopt SOA. The results of this research emphasise the importance of being able to embrace change and innovation and modify strategies in order to reflect the constant changes required for the adoption of SOA. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Authors: Jacobs, Carmen
- Date: 2013-07-26
- Subjects: System analysis -- Data processing , System design -- Data processing , Systems integration , Service-oriented architecture (Computer science) , Education, Higher -- Data processing
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:1153 , http://hdl.handle.net/10962/d1008366 , System analysis -- Data processing , System design -- Data processing , Systems integration , Service-oriented architecture (Computer science) , Education, Higher -- Data processing
- Description: The demand for systems i·ntegration has become more and more significant in higher education as institutions want systems that provide coherent information with data that is up to date and not redundant and can seamlessly support the end user experience. Institutions have become more reliant on information systems to support both administrative functions and those involved in teaching, learning and research, but because each department supports a diverse array of computing platforms and applications, it becomes very difficult to integrate these systems. SOA is classified as an innovative approach to integrating existing systems involving the use of independent services that can be accessed without knowledge of the underlying platform implementation. Unfortunately, the SOA initiative will not be success if it is not understood and used correctly by various applications and systems throughout the organisation. SOA introduces complexity and challenges in systems integration, acceptance, governance, data, development planning, security and external opportunities. If an organisation does not embrace or enable change in each of these areas, it is not ready for the adoption of SOA. This research investigates the systems integration challenge in selected South African universities and explores factors for SOA adoption. The framework for the adoption of SOA comprises seven factors, of which Systems Integration is the most significant and represents an efficient starting point for institutions considering SOA adoption. Acceptance, Governance, Data, Development Planning, Security and External Opportunities are other factors of SOA adoption that require careful and thorough consideration before an institution can successfully adopt SOA. The results of this research emphasise the importance of being able to embrace change and innovation and modify strategies in order to reflect the constant changes required for the adoption of SOA. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
Synthesis and evaluation of novel inhibitors of 1-Deoxy-D-xylolose-5-phosphate reductoisomerase as potential antimalarials
- Authors: Conibear, Anne Claire
- Date: 2013-07-19
- Subjects: Antimalarials -- Development , Malaria -- Chemotherapy , Drug development , Enzyme kinetics , Phosphate esters
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4451 , http://hdl.handle.net/10962/d1008282 , Antimalarials -- Development , Malaria -- Chemotherapy , Drug development , Enzyme kinetics , Phosphate esters
- Description: Malaria continues to be an enormous health-threat in the developing world and the emergence of drug resistance has further compounded the problem. The parasite-specific enzyme, 1-deoxY-D-xylulose-S-phosphate reductoisomerase (DXR), has recently been validated as a promising antimalarial drug target. The present study comprises a combination of synthetic, physical organic, computer modelling and bioassay techniques directed towards the development of novel DXR inhibitors. A range of 2-heteroarylamino-2-oxoethyl- and 2- heteroarylamino-2-oxopropyl phosphonate esters and their corresponding phosphonic acid salts have been synthesised as analogues of the highly active DXR inhibitor, fosmidomycin. Treatment of the heteroarylamino precursors with chloroacetyl chloride or chloropropionyl chloride afforded chloroamide intermediates, Arbuzov reactions of which led to the corresponding diethyl phosphonate esters. Hydrolysis of the esters has been effected using bromotrimethylsilane. Twenty-four new compounds have been prepared and fully characterised using elemental (HRMS or combustion) and spectroscopic (1- and 2-D NMR and IR) analysis. A 31p NMR kinetic study has been carried out on the two-step silylation reaction involved in the hydrolysis of the phosphonate esters and has provided activation parameters for the reaction. The kinetic analysis was refined using a computational method to give an improved fit with the experimental data. Saturation transfer difference (STD) NMR analysis, computer-simulated docking and enzyme inhibition assays have been used to evaluate the enzyme-binding and -inhibition potential of the synthesised ligands. Minimal to moderate inhibitory activity has been observed and several structure-activity relationships have been identified. In silica exploration of the DXR active site has revealed an additional binding pocket and information on the topology of the active site has led to the de novo design of a new series of potential ligands. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Authors: Conibear, Anne Claire
- Date: 2013-07-19
- Subjects: Antimalarials -- Development , Malaria -- Chemotherapy , Drug development , Enzyme kinetics , Phosphate esters
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4451 , http://hdl.handle.net/10962/d1008282 , Antimalarials -- Development , Malaria -- Chemotherapy , Drug development , Enzyme kinetics , Phosphate esters
- Description: Malaria continues to be an enormous health-threat in the developing world and the emergence of drug resistance has further compounded the problem. The parasite-specific enzyme, 1-deoxY-D-xylulose-S-phosphate reductoisomerase (DXR), has recently been validated as a promising antimalarial drug target. The present study comprises a combination of synthetic, physical organic, computer modelling and bioassay techniques directed towards the development of novel DXR inhibitors. A range of 2-heteroarylamino-2-oxoethyl- and 2- heteroarylamino-2-oxopropyl phosphonate esters and their corresponding phosphonic acid salts have been synthesised as analogues of the highly active DXR inhibitor, fosmidomycin. Treatment of the heteroarylamino precursors with chloroacetyl chloride or chloropropionyl chloride afforded chloroamide intermediates, Arbuzov reactions of which led to the corresponding diethyl phosphonate esters. Hydrolysis of the esters has been effected using bromotrimethylsilane. Twenty-four new compounds have been prepared and fully characterised using elemental (HRMS or combustion) and spectroscopic (1- and 2-D NMR and IR) analysis. A 31p NMR kinetic study has been carried out on the two-step silylation reaction involved in the hydrolysis of the phosphonate esters and has provided activation parameters for the reaction. The kinetic analysis was refined using a computational method to give an improved fit with the experimental data. Saturation transfer difference (STD) NMR analysis, computer-simulated docking and enzyme inhibition assays have been used to evaluate the enzyme-binding and -inhibition potential of the synthesised ligands. Minimal to moderate inhibitory activity has been observed and several structure-activity relationships have been identified. In silica exploration of the DXR active site has revealed an additional binding pocket and information on the topology of the active site has led to the de novo design of a new series of potential ligands. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
A comprehensive evaluation framework for system modernization : a case study using data services
- Authors: Barnes, Meredith Anne
- Date: 2011
- Subjects: Computer architecture
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10460 , http://hdl.handle.net/10948/1499 , Computer architecture
- Description: Modernization is a solution to migrate cumbersome existing systems to a new architecture for improved longevity of business processes. Three modernization approaches exist. White-box and black-box modernization are distinct from one another. Grey-box modernization is a hybrid of the white-box and black-box approaches. Modernization can be utilised to create data services for a Service Oriented Architecture. Since it is unclear which modernization approach is more suitable for the development of data services, a comprehensive evaluation framework is proposed to evaluate which of the white- or black-box approaches is more suitable. The comprehensive framework consists of three evaluation components. Firstly, developer effort to modernize existing code is measured by acknowledged software metrics. Secondly, the quality of the data services is measured against identified Quality of Service criteria for data services in particular. Thirdly, the effectiveness of the modernized data services is measured through usability evaluations. By inspection of the combination of application of each of the evaluation components, a recommended approach is identified for the modernization of data services. The comprehensive framework was successfully employed to compare the white-box and black-box modernization approaches applied to a case study. Results indicated that had only a single evaluation component been used, inconclusive results of the more suitable approach may have been obtained. The findings of this research contribute a comprehensive evaluation framework which can be applied to compare modernization approaches and measure modernization success.
- Full Text:
- Date Issued: 2011
- Authors: Barnes, Meredith Anne
- Date: 2011
- Subjects: Computer architecture
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10460 , http://hdl.handle.net/10948/1499 , Computer architecture
- Description: Modernization is a solution to migrate cumbersome existing systems to a new architecture for improved longevity of business processes. Three modernization approaches exist. White-box and black-box modernization are distinct from one another. Grey-box modernization is a hybrid of the white-box and black-box approaches. Modernization can be utilised to create data services for a Service Oriented Architecture. Since it is unclear which modernization approach is more suitable for the development of data services, a comprehensive evaluation framework is proposed to evaluate which of the white- or black-box approaches is more suitable. The comprehensive framework consists of three evaluation components. Firstly, developer effort to modernize existing code is measured by acknowledged software metrics. Secondly, the quality of the data services is measured against identified Quality of Service criteria for data services in particular. Thirdly, the effectiveness of the modernized data services is measured through usability evaluations. By inspection of the combination of application of each of the evaluation components, a recommended approach is identified for the modernization of data services. The comprehensive framework was successfully employed to compare the white-box and black-box modernization approaches applied to a case study. Results indicated that had only a single evaluation component been used, inconclusive results of the more suitable approach may have been obtained. The findings of this research contribute a comprehensive evaluation framework which can be applied to compare modernization approaches and measure modernization success.
- Full Text:
- Date Issued: 2011
a pH-metric speciation and anti-diabetic study of oxovanadium (IV) amino acid derivatives
- Authors: Gundhla, Isaac Zvikomborero
- Date: 2011 , 2011-03-15
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4399 , http://hdl.handle.net/10962/d1006691
- Description: Novel oxovanadium(IV) complexes of glycine, ʟ-alanine, 1-methylimidazole-2-carboxylic acid, imidazole-2-caboxylic acid and imidazole-4-carboxylic acid were synthesized and isolated in the solid state. The ligands and complexes were characterized by elemental analysis, melting point, NMR, IR and UV-vis spectroscopy. The IR studies showed that glycine and ʟ-alanine coordinate monodentately through the amine nitrogen whilst the imidazole-carboxylic acid derivatives assume a bidentate chelation. The electronic spectroscopic studies indicate distorted octahedral geometry for the oxovanadium complexes of amino acids and a square pyramidal geometry for oxovanadium(IV) complexes of imidazole carboxylic acid derivatives. The reaction of vanadyl (VO2+) with glycine, ʟ-alanine, imidazole-2-cabrboxylic acid, imidazole-4-carboxylic acid and 1-methylimidazole-2-carboxylic acid was studied in aqueous solution by pH-potentiometry under oxygen and carbon dioxide-free conditions. The data obtained from these titrations were used to calculate the protonation and stability constants. The results showed that all ligands are suitable for bidentate coordination in the formation of monomeric species although the solid state studies of the oxovanadium(IV) complexes of amino acids showed a monodentate coordination. The overall stability constants for the (VIVO)-ʟ-alanine system (log β120 = 18.27(6)), (VIVO)-glycine system (β120 = 17.22(6)), (VIVO)-imidazole-4-carboxyic acid (β120 = 11.38(8)), (VIVO)-imidazole-2-carboxylic acid (β120 = 11.62(6)) and (VIVO)-1-methylimidazole-2-carboxylic acid (β120 = 15.49(9)) were obtained. The calculations for the species distribution in the experimental pH range showed that the neutral bis-coordinated complexes are dominant over the biological pH range. The glucose uptake effect of oxovanadium(IV) complex of ʟ-alanine, imidazole-4-carboxylic acid, imidazole-2-carboxylic acid and 1-methylimidazole-2-carboxylic acid was investigated using 3T3-L1 adipocytes, Chang liver and C2C12 muscle cells at various concentrations. The compounds had significant glucose uptake on Chang liver cells only at a concentration of 0.1-10 μM whilst in the C2C12 muscle and 3T3-L1 cells the compounds showed little to no activity probably due to the lower concentrations employed as a result of the cytotoxicity of these compounds on these two cell lines.
- Full Text:
- Date Issued: 2011
- Authors: Gundhla, Isaac Zvikomborero
- Date: 2011 , 2011-03-15
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4399 , http://hdl.handle.net/10962/d1006691
- Description: Novel oxovanadium(IV) complexes of glycine, ʟ-alanine, 1-methylimidazole-2-carboxylic acid, imidazole-2-caboxylic acid and imidazole-4-carboxylic acid were synthesized and isolated in the solid state. The ligands and complexes were characterized by elemental analysis, melting point, NMR, IR and UV-vis spectroscopy. The IR studies showed that glycine and ʟ-alanine coordinate monodentately through the amine nitrogen whilst the imidazole-carboxylic acid derivatives assume a bidentate chelation. The electronic spectroscopic studies indicate distorted octahedral geometry for the oxovanadium complexes of amino acids and a square pyramidal geometry for oxovanadium(IV) complexes of imidazole carboxylic acid derivatives. The reaction of vanadyl (VO2+) with glycine, ʟ-alanine, imidazole-2-cabrboxylic acid, imidazole-4-carboxylic acid and 1-methylimidazole-2-carboxylic acid was studied in aqueous solution by pH-potentiometry under oxygen and carbon dioxide-free conditions. The data obtained from these titrations were used to calculate the protonation and stability constants. The results showed that all ligands are suitable for bidentate coordination in the formation of monomeric species although the solid state studies of the oxovanadium(IV) complexes of amino acids showed a monodentate coordination. The overall stability constants for the (VIVO)-ʟ-alanine system (log β120 = 18.27(6)), (VIVO)-glycine system (β120 = 17.22(6)), (VIVO)-imidazole-4-carboxyic acid (β120 = 11.38(8)), (VIVO)-imidazole-2-carboxylic acid (β120 = 11.62(6)) and (VIVO)-1-methylimidazole-2-carboxylic acid (β120 = 15.49(9)) were obtained. The calculations for the species distribution in the experimental pH range showed that the neutral bis-coordinated complexes are dominant over the biological pH range. The glucose uptake effect of oxovanadium(IV) complex of ʟ-alanine, imidazole-4-carboxylic acid, imidazole-2-carboxylic acid and 1-methylimidazole-2-carboxylic acid was investigated using 3T3-L1 adipocytes, Chang liver and C2C12 muscle cells at various concentrations. The compounds had significant glucose uptake on Chang liver cells only at a concentration of 0.1-10 μM whilst in the C2C12 muscle and 3T3-L1 cells the compounds showed little to no activity probably due to the lower concentrations employed as a result of the cytotoxicity of these compounds on these two cell lines.
- Full Text:
- Date Issued: 2011
A platform for computer-assisted multilingual literacy development
- Authors: Mudimba, Bwini Chizabubi
- Date: 2011
- Subjects: FundaWethu , Language acquisition -- Computer-assisted instruction , Language arts (Elementary) -- Computer-assisted instruction , Language and education , Education, Bilingual , Computer-assisted instruction , Educational technology , Computers and literacy
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4600 , http://hdl.handle.net/10962/d1004850 , FundaWethu , Language acquisition -- Computer-assisted instruction , Language arts (Elementary) -- Computer-assisted instruction , Language and education , Education, Bilingual , Computer-assisted instruction , Educational technology , Computers and literacy
- Description: FundaWethu is reading software that is designed to deliver reading lessons to Grade R-3 (foundation phase) children who are learning to read in a multilingual context. Starting from a premise that the system should be both educative and entertaining, the system allows literacy researchers or teachers to construct rich multimedia reading lessons, with text, pictures (possibly animated), and audio files. Using the design-based research methodology which is problem driven and iterative, we followed a user-centred design process in creating FundaWethu. To promote sustainability of the software, we chose to bring teachers on board as “co-designers” using the lesson authoring tool. We made the authoring tool simple enough for use by non computer specialists, but expressive enough to enable a wide range of beginners reading exercises to be constructed in a number of different languages (indigenous South African languages in particular). This project therefore centred on the use of designbased research to build FundaWethu, the design and construction of FundaWethu and the usability study carried out to determine the adequacy of FundaWethu.
- Full Text:
- Date Issued: 2011
- Authors: Mudimba, Bwini Chizabubi
- Date: 2011
- Subjects: FundaWethu , Language acquisition -- Computer-assisted instruction , Language arts (Elementary) -- Computer-assisted instruction , Language and education , Education, Bilingual , Computer-assisted instruction , Educational technology , Computers and literacy
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4600 , http://hdl.handle.net/10962/d1004850 , FundaWethu , Language acquisition -- Computer-assisted instruction , Language arts (Elementary) -- Computer-assisted instruction , Language and education , Education, Bilingual , Computer-assisted instruction , Educational technology , Computers and literacy
- Description: FundaWethu is reading software that is designed to deliver reading lessons to Grade R-3 (foundation phase) children who are learning to read in a multilingual context. Starting from a premise that the system should be both educative and entertaining, the system allows literacy researchers or teachers to construct rich multimedia reading lessons, with text, pictures (possibly animated), and audio files. Using the design-based research methodology which is problem driven and iterative, we followed a user-centred design process in creating FundaWethu. To promote sustainability of the software, we chose to bring teachers on board as “co-designers” using the lesson authoring tool. We made the authoring tool simple enough for use by non computer specialists, but expressive enough to enable a wide range of beginners reading exercises to be constructed in a number of different languages (indigenous South African languages in particular). This project therefore centred on the use of designbased research to build FundaWethu, the design and construction of FundaWethu and the usability study carried out to determine the adequacy of FundaWethu.
- Full Text:
- Date Issued: 2011
A review of the performance of Botswana citizen building contractors
- Authors: Muche, Freeman Hansungule
- Date: 2011
- Subjects: Contractors , Building -- Botswana , Construction industry -- Botswana
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:9685 , http://hdl.handle.net/10948/1336 , Contractors , Building -- Botswana , Construction industry -- Botswana
- Description: Botswana was left underdeveloped in 1966 when it gained its independence from Great Britain. However, with the discovery of diamonds in the late 1970s, the economy of the country changed and two decades later the construction industry had experienced phenomenal growth. A growth propelled by the dominance of foreign contractors and the few citizen contractors available at the time resulted in a competitive environment in the industry. However, citizen contractors were unable to compete on the same level with their foreign counterparts, which was attributable to various factors such as a lack of resources and of business and technical experience. Being a major player in the construction industry, and realising the need to equip and encourage its own base of citizen contractors to actively participate in construction activities, the government of Botswana adopted new measures and policies that were meant to address the imbalances in the construction industry much to the advantage of citizen contractors. The rationale behind this study is to investigate the performance of citizen building contractors following the introduction of preferential procurement policies by the government of Botswana. This research study adopted the descriptive and analytical survey method which entailed the use of questionnaires and a review of the related literature for gathering relevant data. The sample strata included building contractors, consultants, and clients in the Botswana construction industry in Gaborone, Francistown, Selebi-phikwe, Maun, Serowe, and Lobatse. The empirical findings revealed that there is indeed a need to review some of the government assistance programs for citizen contractors in order to avoid the abuse of government resources and to further increase the citizen contractor’s knowledge base, skills, resources and capacity through mentorship and workshops. vi It is therefore incumbent upon government to protect those beneficiaries who genuinely require such assistance by critical assessment of the ineffective policies for the benefit of the whole industry
- Full Text:
- Date Issued: 2011
- Authors: Muche, Freeman Hansungule
- Date: 2011
- Subjects: Contractors , Building -- Botswana , Construction industry -- Botswana
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:9685 , http://hdl.handle.net/10948/1336 , Contractors , Building -- Botswana , Construction industry -- Botswana
- Description: Botswana was left underdeveloped in 1966 when it gained its independence from Great Britain. However, with the discovery of diamonds in the late 1970s, the economy of the country changed and two decades later the construction industry had experienced phenomenal growth. A growth propelled by the dominance of foreign contractors and the few citizen contractors available at the time resulted in a competitive environment in the industry. However, citizen contractors were unable to compete on the same level with their foreign counterparts, which was attributable to various factors such as a lack of resources and of business and technical experience. Being a major player in the construction industry, and realising the need to equip and encourage its own base of citizen contractors to actively participate in construction activities, the government of Botswana adopted new measures and policies that were meant to address the imbalances in the construction industry much to the advantage of citizen contractors. The rationale behind this study is to investigate the performance of citizen building contractors following the introduction of preferential procurement policies by the government of Botswana. This research study adopted the descriptive and analytical survey method which entailed the use of questionnaires and a review of the related literature for gathering relevant data. The sample strata included building contractors, consultants, and clients in the Botswana construction industry in Gaborone, Francistown, Selebi-phikwe, Maun, Serowe, and Lobatse. The empirical findings revealed that there is indeed a need to review some of the government assistance programs for citizen contractors in order to avoid the abuse of government resources and to further increase the citizen contractor’s knowledge base, skills, resources and capacity through mentorship and workshops. vi It is therefore incumbent upon government to protect those beneficiaries who genuinely require such assistance by critical assessment of the ineffective policies for the benefit of the whole industry
- Full Text:
- Date Issued: 2011
A study of a class of invariant optimal control problems on the Euclidean group SE(2)
- Authors: Adams, Ross Montague
- Date: 2011
- Subjects: Matrix groups Lie groups Extremal problems (Mathematics) Maximum principles (Mathematics) Hamilton-Jacobi equations Lyapunov stability
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5420 , http://hdl.handle.net/10962/d1006060
- Description: The aim of this thesis is to study a class of left-invariant optimal control problems on the matrix Lie group SE(2). We classify, under detached feedback equivalence, all controllable (left-invariant) control affine systems on SE(2). This result produces six types of control affine systems on SE(2). Hence, we study six associated left-invariant optimal control problems on SE(2). A left-invariant optimal control problem consists of minimizing a cost functional over the trajectory-control pairs of a left-invariant control system subject to appropriate boundary conditions. Each control problem is lifted from SE(2) to T*SE(2) ≅ SE(2) x se (2)*and then reduced to a problem on se (2)*. The maximum principle is used to obtain the optimal control and Hamiltonian corresponding to the normal extremals. Then we derive the (reduced) extremal equations on se (2)*. These equations are explicitly integrated by trigonometric and Jacobi elliptic functions. Finally, we fully classify, under Lyapunov stability, the equilibrium states of the normal extremal equations for each of the six types under consideration.
- Full Text:
- Date Issued: 2011
- Authors: Adams, Ross Montague
- Date: 2011
- Subjects: Matrix groups Lie groups Extremal problems (Mathematics) Maximum principles (Mathematics) Hamilton-Jacobi equations Lyapunov stability
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5420 , http://hdl.handle.net/10962/d1006060
- Description: The aim of this thesis is to study a class of left-invariant optimal control problems on the matrix Lie group SE(2). We classify, under detached feedback equivalence, all controllable (left-invariant) control affine systems on SE(2). This result produces six types of control affine systems on SE(2). Hence, we study six associated left-invariant optimal control problems on SE(2). A left-invariant optimal control problem consists of minimizing a cost functional over the trajectory-control pairs of a left-invariant control system subject to appropriate boundary conditions. Each control problem is lifted from SE(2) to T*SE(2) ≅ SE(2) x se (2)*and then reduced to a problem on se (2)*. The maximum principle is used to obtain the optimal control and Hamiltonian corresponding to the normal extremals. Then we derive the (reduced) extremal equations on se (2)*. These equations are explicitly integrated by trigonometric and Jacobi elliptic functions. Finally, we fully classify, under Lyapunov stability, the equilibrium states of the normal extremal equations for each of the six types under consideration.
- Full Text:
- Date Issued: 2011
An intelligent multimodal interface for in-car communication systems
- Authors: Sielinou, Patrick Tchankue
- Date: 2011
- Subjects: Automotive telematics , Automobiles -- Electronic equipment
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10461 , http://hdl.handle.net/10948/1495 , Automotive telematics , Automobiles -- Electronic equipment
- Description: In-car communication systems (ICCS) are becoming more frequently used by drivers. ICCS are used in order to minimise the driving distraction due to using a mobile phone while driving. Several usability studies of ICCS utilising speech user interfaces (SUIs) have identified usability issues that can affect the workload, performance, satisfaction and user experience of the driver. This is due to current speech technologies which can be a source of errors that may frustrate the driver and negatively affect the user experience. The aim of this research was to design a new multimodal interface that will manage the interaction between an ICCS and the driver. Unlike the current ICCS, it should make more voice input available, so as to support tasks (e.g. sending text messages; browsing the phone book, etc), which still require a cognitive workload from the driver. An adaptive multimodal interface was proposed in order to address current ICCS issues. The multimodal interface used both speech and manual input; however only the speech channel is used as output. This was done in order to minimise the visual distraction that graphical user interfaces or haptics devices can cause with current ICCS. The adaptive interface was designed to minimise the cognitive distraction of the driver. The adaptive interface ensures that whenever the distraction level of the driver is high, any information communication is postponed. After the design and the implementation of the first version of the prototype interface, called MIMI, a usability evaluation was conducted in order to identify any possible usability issues. Although voice dialling was found to be problematic, the results were encouraging in terms of performance, workload and user satisfaction. The suggestions received from the participants to improve the system usability were incorporated in the next implementation of MIMI. The adaptive module was then implemented to reduce driver distraction based on the driver‟s current context. The proposed architecture showed encouraging results in terms of usability and safety. The adaptive behaviour of MIMI significantly contributed to the reduction of cognitive distraction, because drivers received less information during difficult driving situations.
- Full Text:
- Date Issued: 2011
- Authors: Sielinou, Patrick Tchankue
- Date: 2011
- Subjects: Automotive telematics , Automobiles -- Electronic equipment
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10461 , http://hdl.handle.net/10948/1495 , Automotive telematics , Automobiles -- Electronic equipment
- Description: In-car communication systems (ICCS) are becoming more frequently used by drivers. ICCS are used in order to minimise the driving distraction due to using a mobile phone while driving. Several usability studies of ICCS utilising speech user interfaces (SUIs) have identified usability issues that can affect the workload, performance, satisfaction and user experience of the driver. This is due to current speech technologies which can be a source of errors that may frustrate the driver and negatively affect the user experience. The aim of this research was to design a new multimodal interface that will manage the interaction between an ICCS and the driver. Unlike the current ICCS, it should make more voice input available, so as to support tasks (e.g. sending text messages; browsing the phone book, etc), which still require a cognitive workload from the driver. An adaptive multimodal interface was proposed in order to address current ICCS issues. The multimodal interface used both speech and manual input; however only the speech channel is used as output. This was done in order to minimise the visual distraction that graphical user interfaces or haptics devices can cause with current ICCS. The adaptive interface was designed to minimise the cognitive distraction of the driver. The adaptive interface ensures that whenever the distraction level of the driver is high, any information communication is postponed. After the design and the implementation of the first version of the prototype interface, called MIMI, a usability evaluation was conducted in order to identify any possible usability issues. Although voice dialling was found to be problematic, the results were encouraging in terms of performance, workload and user satisfaction. The suggestions received from the participants to improve the system usability were incorporated in the next implementation of MIMI. The adaptive module was then implemented to reduce driver distraction based on the driver‟s current context. The proposed architecture showed encouraging results in terms of usability and safety. The adaptive behaviour of MIMI significantly contributed to the reduction of cognitive distraction, because drivers received less information during difficult driving situations.
- Full Text:
- Date Issued: 2011
An investigation into the effect of carbon type addictives on the negative electrode during the partial state of charge capacity cycling of lead acid batteries
- Authors: Snyders, Charmelle
- Date: 2011
- Subjects: Lead-acid batteries
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10379 , http://hdl.handle.net/10948/1494 , Lead-acid batteries
- Description: It is well known that a conventional lead acid cell that is exposed to a partial state of charge capacity cycling (PSoCCC) would experience a build-up of irreversible PbSO4 on the negative electrode. This results into a damaged negative electrode due to excessive PbSO4 formation by the typical visual “Venetian Blinds” effect of the active material. This displays the loss of adhesion of the active material with the electrode’s grids thereby making large sections of the material ineffective and reducing the cells useful capacity during high current applications. The addition of certain graphites to the negative paste mix had proven to be successful to reduce this effect. In the first part of the study, the physical and chemical properties of the various additives that are added to the negative electrode paste mix were comparatively studied. This was done to investigate any significant differences between various suppliers that could possibly influence the electrochemical characteristics of the Pb-acid battery performance. This comparative study was done by using the following analytical techniques; BET surface area, laser diffraction particle size, PXRD, TGA-MS and SEM. The study showed that there were no significant differences between the additives supplied from different suppliers except for some anomalies in the usefulness of techniques such as N2 adsorption to study the BET surface area of BaSO4. In order to reduce the sulphation effect from occurring within the Pb-acid battery a number of adjustments are made to the electrode active material. For example, Pb-acid battery manufacturers make use of an inert polymer based material, known as Polymat, to cover the electrode surfaces as part of their continuous electrode pasting process. It is made from a non woven polyester fiber that is applied to the pasted electrodes during the continuous pasting process. In this study the Polymat pasted electrodes has demonstrated a better physical adhesion of the active material to the grid support thereby maintaining the active material’s physical integrity. This however did not reduce the sulphation effect due to the high rate partial state of capacity cycling (HRPSoCCC) test but reduced the physical damage due to the irreversible active material blistering effect. The study investigated what effect the Polymat on the electrodes has on the III battery’s Cold Cranking Ability (CCA) at -18 degree C, the HRPSoCCC cycling and its active material utilization. The study showed that there was little or no differences in the CCA and HRPSoCCC capabilities of cells made with the Polymat when compared to cells without the Polymat, with significant improvement in active material’s adhesion and integrity to the grid wire. This was confirmed by PXRD and SEM analysis. Negative electrodes were made with four types of graphites (natural, flake, expanded and nano fibre) added to the negative paste mixture in order to reduce the effect of sulphation. The study looked at using statistical design of experiment (DoE) principles to investigate the variables (additives) such as different graphites, BaSO4 and Vanisperse to the negative electrode paste mixture where upon measuring the responses (electrochemical tests) a set of controlled experiments were done to study the extent of the variables interaction, dependency and independency on the cells electrochemical properties. This was especially in relation to the improvement of the battery’s ability to work under HRPSoCCC. The statistical analysis showed that there was a notable significant influence of the amounts of vanisperse, BaSO4 and their respective interactions on a number of electrochemical responses, such as the Peukert constant (n), CCA discharge time, material utilization at different discharge rates and the ability to capacity cycle under the simulated HRPSoCCC testing. The study did not suggest an optimized concentration of the additives, but did give an indication that there was a statistical significant trend in certain electrochemical responses with an interaction between the amounts of the additives BaSO4 and Vanisperse. The study also showed that the addition of a small amount of Nano carbon can significantly change the observed crystal morphology of the negative active material and that an improvement in the number of capacity cycles can be achieved during the HRPSoCCC test when compared to the other types of graphite additives.
- Full Text:
- Date Issued: 2011
- Authors: Snyders, Charmelle
- Date: 2011
- Subjects: Lead-acid batteries
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10379 , http://hdl.handle.net/10948/1494 , Lead-acid batteries
- Description: It is well known that a conventional lead acid cell that is exposed to a partial state of charge capacity cycling (PSoCCC) would experience a build-up of irreversible PbSO4 on the negative electrode. This results into a damaged negative electrode due to excessive PbSO4 formation by the typical visual “Venetian Blinds” effect of the active material. This displays the loss of adhesion of the active material with the electrode’s grids thereby making large sections of the material ineffective and reducing the cells useful capacity during high current applications. The addition of certain graphites to the negative paste mix had proven to be successful to reduce this effect. In the first part of the study, the physical and chemical properties of the various additives that are added to the negative electrode paste mix were comparatively studied. This was done to investigate any significant differences between various suppliers that could possibly influence the electrochemical characteristics of the Pb-acid battery performance. This comparative study was done by using the following analytical techniques; BET surface area, laser diffraction particle size, PXRD, TGA-MS and SEM. The study showed that there were no significant differences between the additives supplied from different suppliers except for some anomalies in the usefulness of techniques such as N2 adsorption to study the BET surface area of BaSO4. In order to reduce the sulphation effect from occurring within the Pb-acid battery a number of adjustments are made to the electrode active material. For example, Pb-acid battery manufacturers make use of an inert polymer based material, known as Polymat, to cover the electrode surfaces as part of their continuous electrode pasting process. It is made from a non woven polyester fiber that is applied to the pasted electrodes during the continuous pasting process. In this study the Polymat pasted electrodes has demonstrated a better physical adhesion of the active material to the grid support thereby maintaining the active material’s physical integrity. This however did not reduce the sulphation effect due to the high rate partial state of capacity cycling (HRPSoCCC) test but reduced the physical damage due to the irreversible active material blistering effect. The study investigated what effect the Polymat on the electrodes has on the III battery’s Cold Cranking Ability (CCA) at -18 degree C, the HRPSoCCC cycling and its active material utilization. The study showed that there was little or no differences in the CCA and HRPSoCCC capabilities of cells made with the Polymat when compared to cells without the Polymat, with significant improvement in active material’s adhesion and integrity to the grid wire. This was confirmed by PXRD and SEM analysis. Negative electrodes were made with four types of graphites (natural, flake, expanded and nano fibre) added to the negative paste mixture in order to reduce the effect of sulphation. The study looked at using statistical design of experiment (DoE) principles to investigate the variables (additives) such as different graphites, BaSO4 and Vanisperse to the negative electrode paste mixture where upon measuring the responses (electrochemical tests) a set of controlled experiments were done to study the extent of the variables interaction, dependency and independency on the cells electrochemical properties. This was especially in relation to the improvement of the battery’s ability to work under HRPSoCCC. The statistical analysis showed that there was a notable significant influence of the amounts of vanisperse, BaSO4 and their respective interactions on a number of electrochemical responses, such as the Peukert constant (n), CCA discharge time, material utilization at different discharge rates and the ability to capacity cycle under the simulated HRPSoCCC testing. The study did not suggest an optimized concentration of the additives, but did give an indication that there was a statistical significant trend in certain electrochemical responses with an interaction between the amounts of the additives BaSO4 and Vanisperse. The study also showed that the addition of a small amount of Nano carbon can significantly change the observed crystal morphology of the negative active material and that an improvement in the number of capacity cycles can be achieved during the HRPSoCCC test when compared to the other types of graphite additives.
- Full Text:
- Date Issued: 2011
An investigation into the state-of-practice of information security within Zambian copper mines: a case study
- Authors: Lukweza, Chishala
- Date: 2011
- Subjects: Computer security -- Zambia -- Management Case studies , Computer networks -- Security measures -- Zambia Case studies , Copper mines and mining -- Computer networks -- Security measures -- Zambia Case studies , Information technology -- Zambia
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:1146 , http://hdl.handle.net/10962/d1002776 , Computer security -- Zambia -- Management Case studies , Computer networks -- Security measures -- Zambia Case studies , Copper mines and mining -- Computer networks -- Security measures -- Zambia Case studies , Information technology -- Zambia
- Description: Zambian copper mines have embraced the use of information technologies for strategic operations and competitive advantage. This dependence on these technologies has not only been seen in the physical aspects of business operations but also in the use of information systems such as Enterprise Resource Planning Systems (ERPs) for strategic decision making and increased usage of Industrial Control Systems (ICS’) that are meant to enhance operational efficiency in production areas. A survey was conducted to explore leadership perceptions on information security practices in Zambian copper mines and an ISO/IEC 27002 Audit Tool was administered to middle management in a particular mine for an in-depth analysis of their information security practices. Results revealed that although information security controls may have been put in place in these organisations, there are still areas that require attention. Senior management and middle management have different perceptions as to the extent to which information security practices are conducted in these copper mines. This implies that management may not be fully involved in certain aspects of these organisations’ information security practices. The results concluded that management needs to be fully involved and provide support for information security programs. Furthermore, these information security programs should be standardised so as to effectively protect these organisations’ information assets. This should also include the involvement of personnel as key players in the information security process.
- Full Text:
- Date Issued: 2011
- Authors: Lukweza, Chishala
- Date: 2011
- Subjects: Computer security -- Zambia -- Management Case studies , Computer networks -- Security measures -- Zambia Case studies , Copper mines and mining -- Computer networks -- Security measures -- Zambia Case studies , Information technology -- Zambia
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:1146 , http://hdl.handle.net/10962/d1002776 , Computer security -- Zambia -- Management Case studies , Computer networks -- Security measures -- Zambia Case studies , Copper mines and mining -- Computer networks -- Security measures -- Zambia Case studies , Information technology -- Zambia
- Description: Zambian copper mines have embraced the use of information technologies for strategic operations and competitive advantage. This dependence on these technologies has not only been seen in the physical aspects of business operations but also in the use of information systems such as Enterprise Resource Planning Systems (ERPs) for strategic decision making and increased usage of Industrial Control Systems (ICS’) that are meant to enhance operational efficiency in production areas. A survey was conducted to explore leadership perceptions on information security practices in Zambian copper mines and an ISO/IEC 27002 Audit Tool was administered to middle management in a particular mine for an in-depth analysis of their information security practices. Results revealed that although information security controls may have been put in place in these organisations, there are still areas that require attention. Senior management and middle management have different perceptions as to the extent to which information security practices are conducted in these copper mines. This implies that management may not be fully involved in certain aspects of these organisations’ information security practices. The results concluded that management needs to be fully involved and provide support for information security programs. Furthermore, these information security programs should be standardised so as to effectively protect these organisations’ information assets. This should also include the involvement of personnel as key players in the information security process.
- Full Text:
- Date Issued: 2011
An ion imprinted polymer for the determination of Ni (II) ions from mine tailing samples
- Authors: Rammika, Modise
- Date: 2011
- Subjects: Imprinted polymers , Metal ions , Polymerization , Mineral industries -- Waste disposal
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4323 , http://hdl.handle.net/10962/d1004981 , Imprinted polymers , Metal ions , Polymerization , Mineral industries -- Waste disposal
- Description: A Ni(II)-dimethylglyoxime ion imprinted polymer {Ni(II)-DMG IIP} was synthesized by the trapping method using the bulk polymerisation format. The structures of the imprinted and non-imprinted polymer were evaluated by infrared spectroscopy and the morphology was observed by scanning electron microscopy. The Ni(II)-DMG IIP was optimised for pH, mass, time and by the uniform design experimental method for the molar ratios of monomer to crosslinker to porogen and template to ligands as well as keeping these parameters constant and varying the quantities of initiator, 2,2'-azobisisobutyronitrile (AIBN). The optimum pH was 8.5, optimum mass was 50 mg, optimum time was 1 min and the optimum molar ratios of crosslinker to monomer, monomer to template and nickel(II) sulfate hexahydrate (NiSO₄.6H₂O) to 4-vinylpyridine to dimethylglyoxime were found to be 3.3:1.0, 0.6:1.0 and 1.0:0.6:3.6 respectively with 30 mg and 8 mL as the optimum amounts of initiator and porogen respectively. Through this optimisation, recovery of Ni(II) was increased from 98 to 100%. Selectivity of the ion imprinted polymer was evaluated by analysing, using an inductively coupled plasma-optical emission spectrometer, for Ni(II) ions that were spiked with varying concentrations of Co(II), Cu(II), Zn(II), Pd(II), Fe(II), Ca(II), Mg(II), Na(I) and K(I) in aqueous samples. Selectivity studies also confirmed that the ion imprinted polymer had very good selectivity characterised by % RSD of less than 5 %. Co(II) was the only ion found to slightly interfere with the determination of Ni(II). The limits of detection and quantification were found to be 3x10⁻⁴ μg/mL and 9x10⁻⁴ μg/mL respectively. The method was evaluated by a custom solution of ground water certified reference material (SEP-3) and sandy soil reference material (BCR-142R) and the concentrations of Ni(II) obtained were not significantly different to the certified ones. The Ni(II)-DMG IIP was then evaluated in aqueous and soil samples where recoveries of 93 to 100% and 98 to 99% respectively were obtained with enrichment factors ranging from 2 to 18 in aqueous and 27 to 40 in soil samples. Finally, the Ni(II)-DMG IIP was used to analyse mine tailings samples and Ni(II) recovery of 99% was obtained with an enrichment factor of 2.
- Full Text:
- Date Issued: 2011
- Authors: Rammika, Modise
- Date: 2011
- Subjects: Imprinted polymers , Metal ions , Polymerization , Mineral industries -- Waste disposal
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4323 , http://hdl.handle.net/10962/d1004981 , Imprinted polymers , Metal ions , Polymerization , Mineral industries -- Waste disposal
- Description: A Ni(II)-dimethylglyoxime ion imprinted polymer {Ni(II)-DMG IIP} was synthesized by the trapping method using the bulk polymerisation format. The structures of the imprinted and non-imprinted polymer were evaluated by infrared spectroscopy and the morphology was observed by scanning electron microscopy. The Ni(II)-DMG IIP was optimised for pH, mass, time and by the uniform design experimental method for the molar ratios of monomer to crosslinker to porogen and template to ligands as well as keeping these parameters constant and varying the quantities of initiator, 2,2'-azobisisobutyronitrile (AIBN). The optimum pH was 8.5, optimum mass was 50 mg, optimum time was 1 min and the optimum molar ratios of crosslinker to monomer, monomer to template and nickel(II) sulfate hexahydrate (NiSO₄.6H₂O) to 4-vinylpyridine to dimethylglyoxime were found to be 3.3:1.0, 0.6:1.0 and 1.0:0.6:3.6 respectively with 30 mg and 8 mL as the optimum amounts of initiator and porogen respectively. Through this optimisation, recovery of Ni(II) was increased from 98 to 100%. Selectivity of the ion imprinted polymer was evaluated by analysing, using an inductively coupled plasma-optical emission spectrometer, for Ni(II) ions that were spiked with varying concentrations of Co(II), Cu(II), Zn(II), Pd(II), Fe(II), Ca(II), Mg(II), Na(I) and K(I) in aqueous samples. Selectivity studies also confirmed that the ion imprinted polymer had very good selectivity characterised by % RSD of less than 5 %. Co(II) was the only ion found to slightly interfere with the determination of Ni(II). The limits of detection and quantification were found to be 3x10⁻⁴ μg/mL and 9x10⁻⁴ μg/mL respectively. The method was evaluated by a custom solution of ground water certified reference material (SEP-3) and sandy soil reference material (BCR-142R) and the concentrations of Ni(II) obtained were not significantly different to the certified ones. The Ni(II)-DMG IIP was then evaluated in aqueous and soil samples where recoveries of 93 to 100% and 98 to 99% respectively were obtained with enrichment factors ranging from 2 to 18 in aqueous and 27 to 40 in soil samples. Finally, the Ni(II)-DMG IIP was used to analyse mine tailings samples and Ni(II) recovery of 99% was obtained with an enrichment factor of 2.
- Full Text:
- Date Issued: 2011
Antibacterial activity of liposome encapsulated cyclo(TYR-PRO)
- Authors: Tshanga, Siphokazi Sisanda
- Date: 2011
- Subjects: Peptide antibiotics , Antibacterial agents -- Therapeutic use -- Testing
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10132 , http://hdl.handle.net/10948/1450 , Peptide antibiotics , Antibacterial agents -- Therapeutic use -- Testing
- Description: Cyclic dipeptides (CDPs) are amino acid-based compounds, some of which possess antibacterial activity. The encapsulation of certain drugs into liposomes has been found to improve their activity in terms of bioavailability and duration of action. Liposomes are small vesicles that are under investigation as drug carriers for the delivery of therapeutic agents. A number of liposome formulations are currently under clinical trial review, whilst some have already been approved for clinical use. The aim of this study was to optimize a liposomal cyclo(Tyr-Pro) formulation and to assess its antibacterial activity against various Gram-positive and Gram-negative bacteria. Response surface methodology (RSM) using the central composite design (CCD) model was used to optimize liposomal formulations of cyclo(Tyr-Pro) for each of the four bacteria, namely Bacillus cereus, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Percent drug encapsulated and bacterial inhibition were investigated with respect to two independent variables, i.e. lipid composition and cholesterol content. Design Expert 8 was used for the purpose of finding the combination of independent variables that would yield an optimal formulation for each bacterium. The model selected by the software failed to adequately correlate the predicted models to the experimental data. The in vitro experiments showed that the antibacterial activity of liposome-encapsulated cyclo(Tyr-Pro) was superior to that of its free counterpart. Binding maximum or Bmax for the encapsulated compound at concentrations as low as 0.412 mg/ml, was significantly higher than that obtained for free cyclo(Tyr-Pro) which was tested at a concentration of 20 mg/ml. Furthermore, encapsulation of cyclo(Tyr-Pro) into a liposome formulation enhanced its potency. This was evident in the lower IC50 values for the liposomal compound when compared to free cyclo(Tyr-Pro).
- Full Text:
- Date Issued: 2011
- Authors: Tshanga, Siphokazi Sisanda
- Date: 2011
- Subjects: Peptide antibiotics , Antibacterial agents -- Therapeutic use -- Testing
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10132 , http://hdl.handle.net/10948/1450 , Peptide antibiotics , Antibacterial agents -- Therapeutic use -- Testing
- Description: Cyclic dipeptides (CDPs) are amino acid-based compounds, some of which possess antibacterial activity. The encapsulation of certain drugs into liposomes has been found to improve their activity in terms of bioavailability and duration of action. Liposomes are small vesicles that are under investigation as drug carriers for the delivery of therapeutic agents. A number of liposome formulations are currently under clinical trial review, whilst some have already been approved for clinical use. The aim of this study was to optimize a liposomal cyclo(Tyr-Pro) formulation and to assess its antibacterial activity against various Gram-positive and Gram-negative bacteria. Response surface methodology (RSM) using the central composite design (CCD) model was used to optimize liposomal formulations of cyclo(Tyr-Pro) for each of the four bacteria, namely Bacillus cereus, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Percent drug encapsulated and bacterial inhibition were investigated with respect to two independent variables, i.e. lipid composition and cholesterol content. Design Expert 8 was used for the purpose of finding the combination of independent variables that would yield an optimal formulation for each bacterium. The model selected by the software failed to adequately correlate the predicted models to the experimental data. The in vitro experiments showed that the antibacterial activity of liposome-encapsulated cyclo(Tyr-Pro) was superior to that of its free counterpart. Binding maximum or Bmax for the encapsulated compound at concentrations as low as 0.412 mg/ml, was significantly higher than that obtained for free cyclo(Tyr-Pro) which was tested at a concentration of 20 mg/ml. Furthermore, encapsulation of cyclo(Tyr-Pro) into a liposome formulation enhanced its potency. This was evident in the lower IC50 values for the liposomal compound when compared to free cyclo(Tyr-Pro).
- Full Text:
- Date Issued: 2011
Applicability of public-private partnership in provision of water infrastructure to central coastal Namibia mines
- Kavela, Lukas Tangeni Taukondjo
- Authors: Kavela, Lukas Tangeni Taukondjo
- Date: 2011
- Subjects: Water-supply -- Namibia , Public-private sector cooperation -- Namibia -- Water-supply
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:9701 , http://hdl.handle.net/10948/d1015641
- Description: This study investigated the potential application of Public-Private Partnerships (PPPs) in water supply, taking cognisance of the water demand and supply in the Central Coastal Namibian (CCN) mines. NamWater does not meet demand for the existing and planned mines in the CCN. Primary objective was to investigate and analyse water demand and supply as well as the effectiveness of PPP as a solution by exploring its potential, successes and challenges. In order to obtain relevant information, literature review was used to collect evidence on water demand and supply in the north-central Namibia as well as the application of PPP in provision of water supply. Multi-case studies were carried out on three operational mines, namely; Rio Tinto Rössing Uranium Mine, Langer Heinrich Uranium Mine and Areva Trekkopje Uranium Mine to attain empirical data on water supply requirements. A self-administered questionnaire was used to collect the data supplemented by interviews with NamWater, operational mines within the study area, government institutions, and knowledgeable consultants on water and the mining environment. The key findings included: institutional weaknesses and lack of legislation frameworks to support PPP projects; skills shortage; and financing problems were identified as major challenges. Results further show that these problems could be resolved by effectively implementing a PPP approach, whereby the Special Purpose Vehicle (SPV) enters into a contract with the public authority. The study concludes that there is a need to increase water output to meet current and future supply requirements to these mines. This situation should be addressed by all stakeholders, from government to private sectors. The study recommends that a sea water desalination plant should be developed to increase water capacity supply to the existing operational and prospective mines in the area. The PPP approach is proposed as a possible solution to water supply infrastructure service delivery problem to ensure long-term water security in the CCN mines.
- Full Text:
- Date Issued: 2011
- Authors: Kavela, Lukas Tangeni Taukondjo
- Date: 2011
- Subjects: Water-supply -- Namibia , Public-private sector cooperation -- Namibia -- Water-supply
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:9701 , http://hdl.handle.net/10948/d1015641
- Description: This study investigated the potential application of Public-Private Partnerships (PPPs) in water supply, taking cognisance of the water demand and supply in the Central Coastal Namibian (CCN) mines. NamWater does not meet demand for the existing and planned mines in the CCN. Primary objective was to investigate and analyse water demand and supply as well as the effectiveness of PPP as a solution by exploring its potential, successes and challenges. In order to obtain relevant information, literature review was used to collect evidence on water demand and supply in the north-central Namibia as well as the application of PPP in provision of water supply. Multi-case studies were carried out on three operational mines, namely; Rio Tinto Rössing Uranium Mine, Langer Heinrich Uranium Mine and Areva Trekkopje Uranium Mine to attain empirical data on water supply requirements. A self-administered questionnaire was used to collect the data supplemented by interviews with NamWater, operational mines within the study area, government institutions, and knowledgeable consultants on water and the mining environment. The key findings included: institutional weaknesses and lack of legislation frameworks to support PPP projects; skills shortage; and financing problems were identified as major challenges. Results further show that these problems could be resolved by effectively implementing a PPP approach, whereby the Special Purpose Vehicle (SPV) enters into a contract with the public authority. The study concludes that there is a need to increase water output to meet current and future supply requirements to these mines. This situation should be addressed by all stakeholders, from government to private sectors. The study recommends that a sea water desalination plant should be developed to increase water capacity supply to the existing operational and prospective mines in the area. The PPP approach is proposed as a possible solution to water supply infrastructure service delivery problem to ensure long-term water security in the CCN mines.
- Full Text:
- Date Issued: 2011
Application of catalysts and nanomaterials in the design of an electrochemical sensor for ochratoxin A
- Authors: Flanagan, Shane Patrick
- Date: 2011 , 2010-12-06
- Subjects: Ochratoxins , Filamentous fungi , Electrochemical sensors , Nanostructured materials , Catalysts , Food contamination
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4121 , http://hdl.handle.net/10962/d1013328
- Description: Ochratoxin A is the most potent chlorinated derivative of the ochratoxin group, consisting of a 5'-chlorinated dihydroisocoumarin moiety linked by an amide bond to l-phenylalanine. Produced as a secondary fungal metabolite by several species of Aspergillus and Penicillium, ochratoxin A has been shown to readily contaminate a large variety of commodities including cereals, groundnuts, dried fruit, spices and coffee. This has led to widespread contamination of ochratoxin in wine, beer, milk and meat products. As ochratoxin A is a potent nephrotoxin exhibiting teratogenic and carcinogenic properties, the development of a rapid screening platform for the cost effective control of ochratoxin A content in foodstuffs is therefore required. The evaluation of metallophthalocyanine and carbon nanotube electrode modification toward the development of a nanostructured biosensor capable of enhancing the electrochemical detection of ochratoxin A in complex media is presented. Cyclic voltammetry at a glassy carbon electrode allowed for the optimization of detection parameters including pH and type of supporting electrolyte. Britton-Robinson buffer was found to be the most suitable supporting electrolyte in terms of sensitivity and reproducibility obtaining a LOD of 0.28 μM as determined by differential pulse voltammetry. Subsequent analysis determined the dependence of OTA oxidation on pH in acidic media which proceeds with the transfer of two electrons to form a quinone/hydroquinone couple shown to adsorb to the electrode surface. Passivation of the electrode through adsorption of oxidation products was shown to severely limit the detection of OTA upon successive detection cycles. Comparison of various metallophthalocyanine modifiers showed an increase in sensitivity toward the detection of OTA at phthalocyanine complexes with metal based redox processes. However with the exception of NiPc and CoTCPc complexes, phthalocyanine modification was limited by the increase in deviation of current response and extent of fouling. NiPc modification showed an increase in sensitivity by two fold with fouling characteristics comparable to an unmodified electrode while low improvements in fouling was observed at CoTCPc modified electrodes with sensitivity in detection comparable to an unmodified electrode.Modification of the electrode with multi- and single walled carbon nanotubes produced a significant increase in sensitivity toward the detection of ochratoxin A. The electrocatalytic activity of nanotube modifiers was attributed to the increase in surface area and to the addition of oxygenated functional groups upon acid treatment as confirmed by Raman spectroscopy. Acid functionalization of the carbon nanotubes for a period of two hours produced the greatest increase in sensitivity obtaining a respective LOD of 0.09 μM and 0.03 μM for analysis of ochratoxin A at multi- and single walled carbon nanotube modified electrodes. Centrifugal purification of carbon nanotubes was deemed necessary to improve the electrocatalytic activity of the nanotube modifiers through the removal of carbonaceous impurities as visualized by atomic force microscopy. Furthermore, a crude lipase preparation, lipase A, was investigated as a potential biological recognition element for selective detection of ochratoxin A in complex media. Lipase A enabled the hydrolysis of ochratoxin A to the electroactive species ochratoxin α as confirmed by thin layer chromatography and voltammetric analysis. Additional isolation of a pure hydrolase from the lipase A preparation is required prior to utilization within a nanostructured biosensor platform capable of detecting ochratoxin A in complex media.
- Full Text:
- Date Issued: 2011
- Authors: Flanagan, Shane Patrick
- Date: 2011 , 2010-12-06
- Subjects: Ochratoxins , Filamentous fungi , Electrochemical sensors , Nanostructured materials , Catalysts , Food contamination
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4121 , http://hdl.handle.net/10962/d1013328
- Description: Ochratoxin A is the most potent chlorinated derivative of the ochratoxin group, consisting of a 5'-chlorinated dihydroisocoumarin moiety linked by an amide bond to l-phenylalanine. Produced as a secondary fungal metabolite by several species of Aspergillus and Penicillium, ochratoxin A has been shown to readily contaminate a large variety of commodities including cereals, groundnuts, dried fruit, spices and coffee. This has led to widespread contamination of ochratoxin in wine, beer, milk and meat products. As ochratoxin A is a potent nephrotoxin exhibiting teratogenic and carcinogenic properties, the development of a rapid screening platform for the cost effective control of ochratoxin A content in foodstuffs is therefore required. The evaluation of metallophthalocyanine and carbon nanotube electrode modification toward the development of a nanostructured biosensor capable of enhancing the electrochemical detection of ochratoxin A in complex media is presented. Cyclic voltammetry at a glassy carbon electrode allowed for the optimization of detection parameters including pH and type of supporting electrolyte. Britton-Robinson buffer was found to be the most suitable supporting electrolyte in terms of sensitivity and reproducibility obtaining a LOD of 0.28 μM as determined by differential pulse voltammetry. Subsequent analysis determined the dependence of OTA oxidation on pH in acidic media which proceeds with the transfer of two electrons to form a quinone/hydroquinone couple shown to adsorb to the electrode surface. Passivation of the electrode through adsorption of oxidation products was shown to severely limit the detection of OTA upon successive detection cycles. Comparison of various metallophthalocyanine modifiers showed an increase in sensitivity toward the detection of OTA at phthalocyanine complexes with metal based redox processes. However with the exception of NiPc and CoTCPc complexes, phthalocyanine modification was limited by the increase in deviation of current response and extent of fouling. NiPc modification showed an increase in sensitivity by two fold with fouling characteristics comparable to an unmodified electrode while low improvements in fouling was observed at CoTCPc modified electrodes with sensitivity in detection comparable to an unmodified electrode.Modification of the electrode with multi- and single walled carbon nanotubes produced a significant increase in sensitivity toward the detection of ochratoxin A. The electrocatalytic activity of nanotube modifiers was attributed to the increase in surface area and to the addition of oxygenated functional groups upon acid treatment as confirmed by Raman spectroscopy. Acid functionalization of the carbon nanotubes for a period of two hours produced the greatest increase in sensitivity obtaining a respective LOD of 0.09 μM and 0.03 μM for analysis of ochratoxin A at multi- and single walled carbon nanotube modified electrodes. Centrifugal purification of carbon nanotubes was deemed necessary to improve the electrocatalytic activity of the nanotube modifiers through the removal of carbonaceous impurities as visualized by atomic force microscopy. Furthermore, a crude lipase preparation, lipase A, was investigated as a potential biological recognition element for selective detection of ochratoxin A in complex media. Lipase A enabled the hydrolysis of ochratoxin A to the electroactive species ochratoxin α as confirmed by thin layer chromatography and voltammetric analysis. Additional isolation of a pure hydrolase from the lipase A preparation is required prior to utilization within a nanostructured biosensor platform capable of detecting ochratoxin A in complex media.
- Full Text:
- Date Issued: 2011
Application of macroinvertebrate based biomonitoring approaches to assess anthropogenic impacts in the Swartkops River, South Africa
- Authors: Odume, Oghenekaro Nelson
- Date: 2011
- Subjects: Water -- Pollution -- South Africa -- Swartkops River Water quality biological assessment -- South Africa -- Swartkops River Environmental monitoring -- South Africa -- Swartkops River Aquatic invertebrates -- Effect of water pollution on -- South Africa -- Swartkops River
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:6040 , http://hdl.handle.net/10962/d1006199
- Description: A growing human population accompanied by urbanisation and industrialisation have led to over exploitation and pollution of freshwater resources and have consequently impacted on aquatic ecosystem health. The Swartkops River in the Eastern Cape of South Africa is no exception. It drains a heavily industrialised catchment which has led to deterioration of its water quality due to pollution. Integrated water resources management (IWRM) requires the concurrent sustainable use of water resources and the protection of aquatic ecosystem health. Macroinvertebrates are well known for their ability to reflect the health of the environment in which they live, thus they were used to assess anthropogenic impacts in the Swartkops River for this study. Macroinvertebrate based biomonitoring approaches, including the South African Scoring System version 5 (SASS5); a multimetric approach involving 19 metrics; Chironomidae community assessments and screening of morphological deformities in Chironomidae larvae, were applied at four selected sampling sites to assess environmental water quality in the Swartkops River. Macroinvertebrates were sampled us ing the SASS5 protocols. Chironomidae were mounted and identified as far as practically possible using available keys. Mentum, ligula, mandible, paraligula and antenna in Chironomidae larvae were screened for deformities. Physical and chemical water quality variables were measured at each of the selected sampling sites. All data were subjected to relevant statistical analyses. Of the four sites sampled during the study period, results revealed that water quality at site 1 was the least impacted with highest SASS5 scores, average score per taxa (ASPT) values, richness, diversity, equitability and Ephemeroptera –Plecoptera-Trichoptera (EPT) richness, as well as least incidences of chironomid deformities. Water quality at site 2 was considered the next least impacted with higher SASS5 scores, A SPT values, richness, diversity and equitability, and lower incidences of deformities compared to sites 3 and 4. SASS5 scores and ASPT values revealed that both sites 3 and 4 were critically modified but the multimetric analysis, Chironomidae community assessment and incidences of deformities in Chironomidae larvae indicated that site 3 is the most impacted of the four sampling sites, with least species diversity, richness, equitability and highest incidences of deformities. The study revealed the importance of multicriteria approach to environmental biomonitoring as an integrated water resources management tool, and based on the results, site 3, as the most impacted, could be prioritised for restoration intervention.
- Full Text:
- Date Issued: 2011
- Authors: Odume, Oghenekaro Nelson
- Date: 2011
- Subjects: Water -- Pollution -- South Africa -- Swartkops River Water quality biological assessment -- South Africa -- Swartkops River Environmental monitoring -- South Africa -- Swartkops River Aquatic invertebrates -- Effect of water pollution on -- South Africa -- Swartkops River
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:6040 , http://hdl.handle.net/10962/d1006199
- Description: A growing human population accompanied by urbanisation and industrialisation have led to over exploitation and pollution of freshwater resources and have consequently impacted on aquatic ecosystem health. The Swartkops River in the Eastern Cape of South Africa is no exception. It drains a heavily industrialised catchment which has led to deterioration of its water quality due to pollution. Integrated water resources management (IWRM) requires the concurrent sustainable use of water resources and the protection of aquatic ecosystem health. Macroinvertebrates are well known for their ability to reflect the health of the environment in which they live, thus they were used to assess anthropogenic impacts in the Swartkops River for this study. Macroinvertebrate based biomonitoring approaches, including the South African Scoring System version 5 (SASS5); a multimetric approach involving 19 metrics; Chironomidae community assessments and screening of morphological deformities in Chironomidae larvae, were applied at four selected sampling sites to assess environmental water quality in the Swartkops River. Macroinvertebrates were sampled us ing the SASS5 protocols. Chironomidae were mounted and identified as far as practically possible using available keys. Mentum, ligula, mandible, paraligula and antenna in Chironomidae larvae were screened for deformities. Physical and chemical water quality variables were measured at each of the selected sampling sites. All data were subjected to relevant statistical analyses. Of the four sites sampled during the study period, results revealed that water quality at site 1 was the least impacted with highest SASS5 scores, average score per taxa (ASPT) values, richness, diversity, equitability and Ephemeroptera –Plecoptera-Trichoptera (EPT) richness, as well as least incidences of chironomid deformities. Water quality at site 2 was considered the next least impacted with higher SASS5 scores, A SPT values, richness, diversity and equitability, and lower incidences of deformities compared to sites 3 and 4. SASS5 scores and ASPT values revealed that both sites 3 and 4 were critically modified but the multimetric analysis, Chironomidae community assessment and incidences of deformities in Chironomidae larvae indicated that site 3 is the most impacted of the four sampling sites, with least species diversity, richness, equitability and highest incidences of deformities. The study revealed the importance of multicriteria approach to environmental biomonitoring as an integrated water resources management tool, and based on the results, site 3, as the most impacted, could be prioritised for restoration intervention.
- Full Text:
- Date Issued: 2011
Assessing the statistical methodologies of business research in the South African context
- Authors: Ndou, Aifheli Amos
- Date: 2011
- Subjects: Statistics -- South Africa , Commercial statistics -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:8631 , http://hdl.handle.net/10948/1484 , Statistics -- South Africa , Commercial statistics -- South Africa
- Description: The aim of the study is to establish an acceptable classification scheme for the statistical methods used in business research. The approach compares the statistical component of the research and evaluates how it has changed over time and across different journals. If, as expected, the statistical expertise has changed, the change would be identified with the view to recommending curriculum changes for Statistics Departments of South African tertiary institutions.
- Full Text:
- Date Issued: 2011
- Authors: Ndou, Aifheli Amos
- Date: 2011
- Subjects: Statistics -- South Africa , Commercial statistics -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:8631 , http://hdl.handle.net/10948/1484 , Statistics -- South Africa , Commercial statistics -- South Africa
- Description: The aim of the study is to establish an acceptable classification scheme for the statistical methods used in business research. The approach compares the statistical component of the research and evaluates how it has changed over time and across different journals. If, as expected, the statistical expertise has changed, the change would be identified with the view to recommending curriculum changes for Statistics Departments of South African tertiary institutions.
- Full Text:
- Date Issued: 2011
Assessment of organochlorine pesticide residues in fish samples from the Okavango Delta, Botswana
- Authors: Mpofu, Christopher
- Date: 2011 , 2011-02-28
- Subjects: Pesticides , Organochlorine compounds , Freshwater fishes -- Effect of pesticides on -- Botswana -- Okavango River Delta , Water -- Pesticide content -- Botswana -- Okavango River Delta , Catfishes -- Botswana -- Okavango River Delta , Tigerfish -- Botswana -- Okavango River Delta , Redbreast tilapia -- Botswana -- Okavango River Delta
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4404 , http://hdl.handle.net/10962/d1006707 , Pesticides , Organochlorine compounds , Freshwater fishes -- Effect of pesticides on -- Botswana -- Okavango River Delta , Water -- Pesticide content -- Botswana -- Okavango River Delta , Catfishes -- Botswana -- Okavango River Delta , Tigerfish -- Botswana -- Okavango River Delta , Redbreast tilapia -- Botswana -- Okavango River Delta
- Description: This thesis presents an evaluation of the dispersive solid-phase extraction (d-SPE) method referred to as the quick, easy, cheap, effective, rugged and safe (QuEChERS) method for the determination of four organochlorine pesticide residues in fish samples. The pesticides investigated in this study were o, p′-DDT, p, p′-DDE, aldrin and dieldrin. The combined use of Gas Chromatography with an Electron Capture Detector (GC-ECD) and sensitive Time of Flight (TOF) mass detector facilitated the identification of the target analytes. In the absence of certified reference material, the overall analytical procedure was validated by systematic recovery experiments on spiked samples at three levels of 2, 5 and 10 ng/g. The targeted compounds were successfully extracted and their recovery ranged from 76 to 96% with relative standard deviations of less than 13%. The optimum QuEChERS conditions were 2g of fish powder, 10 ml acetonitrile and 1 min shaking time. The optimal conditions were applied to assess the levels of chlorinated pesticides in blunt-tooth catfish (Clarias ngamensis), tigerfish (Hydrocynus vittatus), Oreochromis andersonii and red-breasted tilapia (Tilapia rendalli) from the Okavango Delta, Botswana. Dieldrin, p, p′-DDE and aldrin were detected in all the analysed samples with a concentration range of 0.04 – 0.29, 0.07 – 0.33, 0.04 – 0.28 and 0.03 – 0.24 ng/g per dry weight in O. andersonii, C. ngamensis, T. rendalli and H. vittatus respectively. These concentrations were below the US-EPA 0.1 μg/g allowable limit in edible fish and the Australian Maximum Residue Limit (MRL) of 50 - 1000 ng/g in fresh fish. DDT was not detected in all the fish species investigated. The mean lipid content recorded in the fish samples were 1.24, 2.16, 2.18 and 4.21% for H. vittatus, T. rendalli, O. andersonni and C. ngamensis respectively. No systematic trend was observed between fish age and pesticide levels in fish. Acetylcholinesterase (AChE) activity assays were performed to assess the effects of organochlorine pesticides in Clarias ngamensis. The enzyme activity recorded in Clarias ngamensis from the Okavango Delta and the reference site was 12.31 μmol of acetylcholine iodide hydrolysed/min/g brain tissue. The enzyme activity remained the same indicating no enzyme inhibition. The conclusions drawn from this study are that the QuEChERS method is applicable for the determination of organochlorine pesticide residues in fish matrices. The fish from the Okavango Delta are safe for human consumption.
- Full Text:
- Date Issued: 2011
- Authors: Mpofu, Christopher
- Date: 2011 , 2011-02-28
- Subjects: Pesticides , Organochlorine compounds , Freshwater fishes -- Effect of pesticides on -- Botswana -- Okavango River Delta , Water -- Pesticide content -- Botswana -- Okavango River Delta , Catfishes -- Botswana -- Okavango River Delta , Tigerfish -- Botswana -- Okavango River Delta , Redbreast tilapia -- Botswana -- Okavango River Delta
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4404 , http://hdl.handle.net/10962/d1006707 , Pesticides , Organochlorine compounds , Freshwater fishes -- Effect of pesticides on -- Botswana -- Okavango River Delta , Water -- Pesticide content -- Botswana -- Okavango River Delta , Catfishes -- Botswana -- Okavango River Delta , Tigerfish -- Botswana -- Okavango River Delta , Redbreast tilapia -- Botswana -- Okavango River Delta
- Description: This thesis presents an evaluation of the dispersive solid-phase extraction (d-SPE) method referred to as the quick, easy, cheap, effective, rugged and safe (QuEChERS) method for the determination of four organochlorine pesticide residues in fish samples. The pesticides investigated in this study were o, p′-DDT, p, p′-DDE, aldrin and dieldrin. The combined use of Gas Chromatography with an Electron Capture Detector (GC-ECD) and sensitive Time of Flight (TOF) mass detector facilitated the identification of the target analytes. In the absence of certified reference material, the overall analytical procedure was validated by systematic recovery experiments on spiked samples at three levels of 2, 5 and 10 ng/g. The targeted compounds were successfully extracted and their recovery ranged from 76 to 96% with relative standard deviations of less than 13%. The optimum QuEChERS conditions were 2g of fish powder, 10 ml acetonitrile and 1 min shaking time. The optimal conditions were applied to assess the levels of chlorinated pesticides in blunt-tooth catfish (Clarias ngamensis), tigerfish (Hydrocynus vittatus), Oreochromis andersonii and red-breasted tilapia (Tilapia rendalli) from the Okavango Delta, Botswana. Dieldrin, p, p′-DDE and aldrin were detected in all the analysed samples with a concentration range of 0.04 – 0.29, 0.07 – 0.33, 0.04 – 0.28 and 0.03 – 0.24 ng/g per dry weight in O. andersonii, C. ngamensis, T. rendalli and H. vittatus respectively. These concentrations were below the US-EPA 0.1 μg/g allowable limit in edible fish and the Australian Maximum Residue Limit (MRL) of 50 - 1000 ng/g in fresh fish. DDT was not detected in all the fish species investigated. The mean lipid content recorded in the fish samples were 1.24, 2.16, 2.18 and 4.21% for H. vittatus, T. rendalli, O. andersonni and C. ngamensis respectively. No systematic trend was observed between fish age and pesticide levels in fish. Acetylcholinesterase (AChE) activity assays were performed to assess the effects of organochlorine pesticides in Clarias ngamensis. The enzyme activity recorded in Clarias ngamensis from the Okavango Delta and the reference site was 12.31 μmol of acetylcholine iodide hydrolysed/min/g brain tissue. The enzyme activity remained the same indicating no enzyme inhibition. The conclusions drawn from this study are that the QuEChERS method is applicable for the determination of organochlorine pesticide residues in fish matrices. The fish from the Okavango Delta are safe for human consumption.
- Full Text:
- Date Issued: 2011
Benefication of glycerol from algae and vegetable oil
- Authors: Mafu, Lubabalo Rowan
- Date: 2011
- Subjects: Glycerin -- Biotechnology , Biodiesel fuels , Renewable natural resources
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10409 , http://hdl.handle.net/10948/d1011503 , Glycerin -- Biotechnology , Biodiesel fuels , Renewable natural resources
- Description: This research has been directed at furthering the utilization of crude glycerol oversupply formed as a by-product from the biodiesel manufacturing process. Phosphorylation of hydroxyl groups is a synthetic route that was investigated for the conversion of glycerol into a glycerol-phosphate (GPE) ester mixture. The process investigated for the synthesis of a GPE product was based on phosphorylation reaction procedures that were previously reported in the literature. The reaction to convert glycerol into a GPE mixture has been thoroughly investigated and the hydrogen chloride gas formed as a reaction by-product has been optimized. The chemical properties of GPE have been studied and discussed together with a mass balance of the overall glycerol phosphorylation process. The phosphate groups contained in polyhydric phosphate molecules have a potential chelating effect on cations. There are several cations that may be chelated by the phosphate ester group of polyhydric phosphate molecules. These cations include ammonium (NH4+), Potassium (K+), Calcium (Ca2+) etc, which are essential as nutrients in plant fertilizer formulations. This research has investigated the use of a GPE synthesized from glycerol in the laboratory and the use thereof as a phosphorus containing base in the formulation and evaluation of Nitrogen, Phosphorus and Potassium (NPK) containing fertilizer solution, Ammonium-Potassium-Glycerol-Phosphate (APGP) fertilizer solution. The APGP fertilizer solution has further been evaluated by growing two week old tomato seedlings under controlled conditions. The performance of the APGP fertiliser solution has been evaluated using design of experiments by comparison with traditionally used liquidAmmonium-Potassium-Phosphate inorganic fertilizer. This fertilizer solution has been prepared in similar manner as APGP formulation with the difference between them being the source of phosphorus. The results have been evaluated using statistic analysis where a significant difference between the evaluated fertilizer formulations was found. The comparative study of these formulations was monitored by the observed plant weights. A blank treatment was used as a control to determine if a significant difference among these formulations was observed. Anova single factor and t-Test methods (Two-Samples assumed of equal variances) are statistical models that were applied to interpret the observed experimental data with respect to wet and dry weighed masses of tomato seedlings. These methods indicated a confirmed conclusion that there was a significant difference between APPO4 solution and APGP solution. The observed data have shown that the APPO4 solution provided significantly better fertigation performance than APGP solution. Consequently, further investigation has been conducted to determine the cause of the poorer performance of the APGP solution. The further study of the APGP fertilizer solution included nutrient stability testing, biological analysis and other observed physical changes of the APGP solution over time. Biological results have revealed the presence of a Fusarium fungus species that has grown and is suspended in APGP fertilizer solution. This microbe species has been observed to play a vital role in consuming fertilizer nutrients. In addition, the observed abnormal plant growth and nutrient decomposition of the APGP formulation has been proposed to be mostly a result of the pathogenicity of the fusarium fungi species that was suspended in the APGP solution. Further work has been proposed in which the effect of such biological contamination is eliminated through adequate sterilization procedures and the APGP formulation re-evaluated.
- Full Text:
- Date Issued: 2011
- Authors: Mafu, Lubabalo Rowan
- Date: 2011
- Subjects: Glycerin -- Biotechnology , Biodiesel fuels , Renewable natural resources
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10409 , http://hdl.handle.net/10948/d1011503 , Glycerin -- Biotechnology , Biodiesel fuels , Renewable natural resources
- Description: This research has been directed at furthering the utilization of crude glycerol oversupply formed as a by-product from the biodiesel manufacturing process. Phosphorylation of hydroxyl groups is a synthetic route that was investigated for the conversion of glycerol into a glycerol-phosphate (GPE) ester mixture. The process investigated for the synthesis of a GPE product was based on phosphorylation reaction procedures that were previously reported in the literature. The reaction to convert glycerol into a GPE mixture has been thoroughly investigated and the hydrogen chloride gas formed as a reaction by-product has been optimized. The chemical properties of GPE have been studied and discussed together with a mass balance of the overall glycerol phosphorylation process. The phosphate groups contained in polyhydric phosphate molecules have a potential chelating effect on cations. There are several cations that may be chelated by the phosphate ester group of polyhydric phosphate molecules. These cations include ammonium (NH4+), Potassium (K+), Calcium (Ca2+) etc, which are essential as nutrients in plant fertilizer formulations. This research has investigated the use of a GPE synthesized from glycerol in the laboratory and the use thereof as a phosphorus containing base in the formulation and evaluation of Nitrogen, Phosphorus and Potassium (NPK) containing fertilizer solution, Ammonium-Potassium-Glycerol-Phosphate (APGP) fertilizer solution. The APGP fertilizer solution has further been evaluated by growing two week old tomato seedlings under controlled conditions. The performance of the APGP fertiliser solution has been evaluated using design of experiments by comparison with traditionally used liquidAmmonium-Potassium-Phosphate inorganic fertilizer. This fertilizer solution has been prepared in similar manner as APGP formulation with the difference between them being the source of phosphorus. The results have been evaluated using statistic analysis where a significant difference between the evaluated fertilizer formulations was found. The comparative study of these formulations was monitored by the observed plant weights. A blank treatment was used as a control to determine if a significant difference among these formulations was observed. Anova single factor and t-Test methods (Two-Samples assumed of equal variances) are statistical models that were applied to interpret the observed experimental data with respect to wet and dry weighed masses of tomato seedlings. These methods indicated a confirmed conclusion that there was a significant difference between APPO4 solution and APGP solution. The observed data have shown that the APPO4 solution provided significantly better fertigation performance than APGP solution. Consequently, further investigation has been conducted to determine the cause of the poorer performance of the APGP solution. The further study of the APGP fertilizer solution included nutrient stability testing, biological analysis and other observed physical changes of the APGP solution over time. Biological results have revealed the presence of a Fusarium fungus species that has grown and is suspended in APGP fertilizer solution. This microbe species has been observed to play a vital role in consuming fertilizer nutrients. In addition, the observed abnormal plant growth and nutrient decomposition of the APGP formulation has been proposed to be mostly a result of the pathogenicity of the fusarium fungi species that was suspended in the APGP solution. Further work has been proposed in which the effect of such biological contamination is eliminated through adequate sterilization procedures and the APGP formulation re-evaluated.
- Full Text:
- Date Issued: 2011
Biomechanical, physiological and perceptual responses of three different athlete groups to the cycle-run transition
- Authors: Cripwell, Devin Matthew
- Date: 2011
- Subjects: Biomechanics -- Research , Human mechanics -- Research , Cycling -- Physiological aspects -- Research , Running -- Physiological aspects -- Research , Exercise -- Physiological aspects -- Research
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5106 , http://hdl.handle.net/10962/d1005184 , Biomechanics -- Research , Human mechanics -- Research , Cycling -- Physiological aspects -- Research , Running -- Physiological aspects -- Research , Exercise -- Physiological aspects -- Research
- Description: The transition from cycling to running has been identified as one of the key determinants of success in triathlon, as it has been suggested that the cycle may affect subsequent running efficiency such that running performance is significantly altered or reduced. It is also suggested that athletes more adapted to the transition itself, rather than purely running or cycling, may be more efficient during the post-cycle running bout. The current study sought to investigate the effects of prior cycling on subsequent selected biomechanical, physiological and perceptual responses of three different athlete groups. Subjects were selected on the basis of their sporting background, and were divided into three groups – triathletes, cyclists and runners. Experimentation required subjects to perform a seven minute treadmill running protocol at 15km.h⁻¹, during which biomechanical (EMG, Stride rate, Stride length, Vertical acceleration), physiological (HR, VO₂, EE) and perceptual (RPE) responses were recorded. After resting, subjects were required to perform a twenty minute stationary cycle at 70% of maximal aerobic power (previously determined), immediately followed by a second seven minute treadmill running protocol during which the same data were collected and compared to those collected during the first run. Biomechanical responses indicate that the cycle protocol had no effect on the muscle activity or vertical acceleration responses of any of the three subject groups, while the triathlete group significantly altered their gait responses in order to preserve running economy. The triathlete group was the least affected when considering the physiological responses, as running economy was preserved for this group. The runner and cyclist groups were significantly affected by the transition, as running economy decreased significantly for these groups. Perceptual responses indicate that athletes more experienced with the transition may find the transition from cycling to running to be easier than those inexperienced in this transition. It is apparent that a high intensity cycle protocol has limited statistical impact on selected biomechanical responses, while physiological and perceptual responses were altered, during a subsequent run, regardless of athlete type. That said, the ability of transition-trained athletes to transition comfortably between disciplines was highlighted, which may have important performance implications.
- Full Text:
- Date Issued: 2011
- Authors: Cripwell, Devin Matthew
- Date: 2011
- Subjects: Biomechanics -- Research , Human mechanics -- Research , Cycling -- Physiological aspects -- Research , Running -- Physiological aspects -- Research , Exercise -- Physiological aspects -- Research
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5106 , http://hdl.handle.net/10962/d1005184 , Biomechanics -- Research , Human mechanics -- Research , Cycling -- Physiological aspects -- Research , Running -- Physiological aspects -- Research , Exercise -- Physiological aspects -- Research
- Description: The transition from cycling to running has been identified as one of the key determinants of success in triathlon, as it has been suggested that the cycle may affect subsequent running efficiency such that running performance is significantly altered or reduced. It is also suggested that athletes more adapted to the transition itself, rather than purely running or cycling, may be more efficient during the post-cycle running bout. The current study sought to investigate the effects of prior cycling on subsequent selected biomechanical, physiological and perceptual responses of three different athlete groups. Subjects were selected on the basis of their sporting background, and were divided into three groups – triathletes, cyclists and runners. Experimentation required subjects to perform a seven minute treadmill running protocol at 15km.h⁻¹, during which biomechanical (EMG, Stride rate, Stride length, Vertical acceleration), physiological (HR, VO₂, EE) and perceptual (RPE) responses were recorded. After resting, subjects were required to perform a twenty minute stationary cycle at 70% of maximal aerobic power (previously determined), immediately followed by a second seven minute treadmill running protocol during which the same data were collected and compared to those collected during the first run. Biomechanical responses indicate that the cycle protocol had no effect on the muscle activity or vertical acceleration responses of any of the three subject groups, while the triathlete group significantly altered their gait responses in order to preserve running economy. The triathlete group was the least affected when considering the physiological responses, as running economy was preserved for this group. The runner and cyclist groups were significantly affected by the transition, as running economy decreased significantly for these groups. Perceptual responses indicate that athletes more experienced with the transition may find the transition from cycling to running to be easier than those inexperienced in this transition. It is apparent that a high intensity cycle protocol has limited statistical impact on selected biomechanical responses, while physiological and perceptual responses were altered, during a subsequent run, regardless of athlete type. That said, the ability of transition-trained athletes to transition comfortably between disciplines was highlighted, which may have important performance implications.
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- Date Issued: 2011