Comparative study of phytochemical composition and insecticidal properties of Lemon species from Eastern Cape and KwaZulu-Natal
- Authors: Nodola, Phumelele
- Date: 2019
- Subjects: Phytochemical -- Insecticidal properties Lemon - - Species – Eastern Cape - - South Africa Citrus limon
- Language: English
- Type: Thesis , Masters , M Sc
- Identifier: http://hdl.handle.net/11260/1348 , vital:32261
- Description: Citrus limon commonly known as lemon is the third most cultivated citrus genus in South Africa mainly cultivated for its juice. This study aims at analysing the chemical composition of Citrus limon waste (leaf and peel) and evaluation of their insecticidal properties. Leaves and peels of Eureka lemons were collected from three South African citrus farms (Addo, Riverside and Mystic), while Lisbon lemons were only collected from two citrus farms (Addo and Riverside) in 2017 and 2018. A total of 36 essential oils were extracted using the Clevenger like apparatus. Analyses of these oils were done using GCMS. An infusion extract of C. limon was prepared according to the standard method and subjected for phytochemical screening to detect and quantify secondary metabolites present. D-Limonene was the major compound identified in all the 36 oils from Eureka and Lisbon peels and leaves (27.9-74.8%). Other dominant compounds identified in Eureka leaf and peel oils were -pinene (5.8-13.2%), -terpinene (4.8-6.7%), geranial (4.0-15.4%), z-citral (6.7-11.7%), sabinene (4.5-6.3%), nerol (4.0-5.4%) and neryl acetate (6.3-9.5%). Some compounds were however present only in some oil such caryophyllene (6.5%) in Mystic dry leaf oil, geraniol (8.0%) in Addo dry leaf oil, as significant compounds. Lisbon leaf and peel essential oils -pinene (4.4- 11.8%), p-cymene (4.2-6.3%), -terpinene (4.4-6.9%), geranial (8.6-17.3%), z-citral (7.1-13.6%), neryl acetate (4.7-9.1%) (5.4-5.9%) were the other prominent peaks identified in the oils. These C. limon essential oils were further tested for insecticidal activities against maize weevils by conducting repellent assay, fumigant assay and contact toxicity; also plant viii powder was used against maize weevils. In general, for all the bioassay, mortality of the insect increased as the contact days with the oil increased. Contact toxicity assays of C. limon peel essential oils were most effective against S. zeamais in which all weevils were dead by day 3 (100% mortality) at 300 μL, while at other concentrations (50, 100, 200 μL) over 50% mortality was observed. The fumigant potential was high with the peel oil with over 50% mortality at 32 and 40 μL in day 4. The oil had moderate repellent effect on the maize weevil. Riverside Eureka dried powder peel was the most effect in all. These results unveil the medicinal potential of South African citrus waste in post-harvest crop protection and good sources of some chemical compounds which are of medicinal value. Furthermore, this study also reveals the economic potential of these wastes in job creation.
- Full Text:
- Date Issued: 2019
- Authors: Nodola, Phumelele
- Date: 2019
- Subjects: Phytochemical -- Insecticidal properties Lemon - - Species – Eastern Cape - - South Africa Citrus limon
- Language: English
- Type: Thesis , Masters , M Sc
- Identifier: http://hdl.handle.net/11260/1348 , vital:32261
- Description: Citrus limon commonly known as lemon is the third most cultivated citrus genus in South Africa mainly cultivated for its juice. This study aims at analysing the chemical composition of Citrus limon waste (leaf and peel) and evaluation of their insecticidal properties. Leaves and peels of Eureka lemons were collected from three South African citrus farms (Addo, Riverside and Mystic), while Lisbon lemons were only collected from two citrus farms (Addo and Riverside) in 2017 and 2018. A total of 36 essential oils were extracted using the Clevenger like apparatus. Analyses of these oils were done using GCMS. An infusion extract of C. limon was prepared according to the standard method and subjected for phytochemical screening to detect and quantify secondary metabolites present. D-Limonene was the major compound identified in all the 36 oils from Eureka and Lisbon peels and leaves (27.9-74.8%). Other dominant compounds identified in Eureka leaf and peel oils were -pinene (5.8-13.2%), -terpinene (4.8-6.7%), geranial (4.0-15.4%), z-citral (6.7-11.7%), sabinene (4.5-6.3%), nerol (4.0-5.4%) and neryl acetate (6.3-9.5%). Some compounds were however present only in some oil such caryophyllene (6.5%) in Mystic dry leaf oil, geraniol (8.0%) in Addo dry leaf oil, as significant compounds. Lisbon leaf and peel essential oils -pinene (4.4- 11.8%), p-cymene (4.2-6.3%), -terpinene (4.4-6.9%), geranial (8.6-17.3%), z-citral (7.1-13.6%), neryl acetate (4.7-9.1%) (5.4-5.9%) were the other prominent peaks identified in the oils. These C. limon essential oils were further tested for insecticidal activities against maize weevils by conducting repellent assay, fumigant assay and contact toxicity; also plant viii powder was used against maize weevils. In general, for all the bioassay, mortality of the insect increased as the contact days with the oil increased. Contact toxicity assays of C. limon peel essential oils were most effective against S. zeamais in which all weevils were dead by day 3 (100% mortality) at 300 μL, while at other concentrations (50, 100, 200 μL) over 50% mortality was observed. The fumigant potential was high with the peel oil with over 50% mortality at 32 and 40 μL in day 4. The oil had moderate repellent effect on the maize weevil. Riverside Eureka dried powder peel was the most effect in all. These results unveil the medicinal potential of South African citrus waste in post-harvest crop protection and good sources of some chemical compounds which are of medicinal value. Furthermore, this study also reveals the economic potential of these wastes in job creation.
- Full Text:
- Date Issued: 2019
Phytochemical Analysis and Biological potential of leaves and peels of three Eastern Cape Citrus Species
- Authors: Matuka, Thandiswa
- Date: 2019
- Subjects: Phytochemical -- Analysis Biology -- Leaves and peels Citrus species -- Eastern Cape -- South Africa
- Language: English
- Type: Thesis , Masters , M Sc
- Identifier: http://hdl.handle.net/11260/1362 , vital:32263
- Description: Citrus sinensis, Citrus reticulata and Citrus clementine are citrus species that belong to a large family Rutaceae. Citrus leaves and peels are often regarded as waste products. In view of the environment pollution impact, the search for end use of citrus waste products for economic value and medicinal purposes was sort after. The aim of this study was to investigate chemical composition of citrus waste products (leaves and peels) essential oils and determine biological active volatile compounds from Citrus sinensis, Citrus reticulata and Citrus clementine Leaves and peels of Citrus sinensis, Citrus reticulata and Citrus clementine collected twice from citrus farm were extracted for essential oils using hydrodistillation method and analysed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) for plant samples collected in 2016 and, gas chromatography (GC) and gas chromatography-triple quadrupole-mass spectrometry (GC-MSMS) for plant samples collected in 2017. The oil yield for Citrus sinensis was 0.19 - 0.85 %v/w for fresh and dried leaf and peels for samples collected in 2016 and 0.26 – 0.87% v/w for those collected in 2017. Analysis of the essential oils analyses identified sabinene (20.4% GCMS, 22.6% GCMSMS), terpinen-4-ol (13.2% GCMS, 6.8% GCMSMS) and linalool (7.6% GCMS, 19.3% GCMSMS) as major compounds in fresh leaves essential oils while β-elemene (16.3% GCMS, 21.4% GCMSMS) and sabinene (10.7% GCMS, 20.8% GCMSMS) were major compounds characterized in dried leaves essential oils. Fresh and dried peels essential oils had limonene as the major constituent (49.6% GCMS, 87.1% GCMSMS) and (73.6% GCMS, 88.3% GCMSMS) respectively. Citrus reticulata essential oils percentage yield range between 0.44-0.80% v/w for fresh and dried leaf and peel oils. Analyses of the GCMS and GCMSMS chromatogram of the volatile oils analyses revealed linalool (33.4% GCMS, 19.6% GCMSMS), (+)-spathulenol (15.2% GCMS, 10.1% GCMSMS) and terpinene-4-ol (4.5% GCMS, 11.9% GCMSMS) as major compounds in fresh leaf essential oils while, sabinene (20.8% GCMS, 32.5% GCMSMS) and linalool (17.2% GCMS, 20.4% GCMSMS) were the major compounds found in dried leaf essential oils. Limonene (55.2%, 75.4% GCMS; 39.5%, 80.8% GCMSMS) was the major prominent compound identified in the C. reticulata peel essential oils in fresh and dried peels respectively, followed by linalool (12.8%, 2.9% GCMS; 48.4%, 7.4% GCMSMS). Percentage yields for the essential oils from C. clementine fresh and dried leaves and peels ranges from 0.40-0.62% v/w for. Fresh and dried leaves volatile oils had sabinene (22.7-27.1%) for GCMS and GCMSMS respectively), linalool (18.5- 20.9% GCMS and GCMSMS) and terpinene-4-ol (6.5- 11.8% GCMS and GCMSMS) as main compounds while fresh and dried peels oils were dominated by limonene (38.9- 86.5% GCMS and GCMSMS). Phytochemical screening experiment of the three citrus species leaves and peels aqueous extract reveal the prominent presence of flavonoids and phenolic compounds in high concentrations. C. reticulata and C. clementine peels and leaves volatile oils exhibited higher antioxidant activity as DPPH free radical scavengers and Ferric reducing power (FRAP) ability, when compared to C. sinensis essential oils which exhibited minimum antioxidant activity. Antioxidant activity of leaves and peels essential oils maybe due to the presence of oxygenated monoterpenes and high limonene content. C. sinensis peels oils showed non-toxic to extremely very low toxicity effect at highest dose 2000 mg/kg. C. reticulata and C. clementine peels oils showed toxicity effect in mice at high dose levels. It is suggested that citrus volatile oils could be safe to use at lower dose levels. Citrus species peels and leaves volatile compounds limonene linalool and sabinene exhibited antiinflammatory activity by significantly reducing rats paw oedema size induced by fresh egg albumin. The study suggests the use of Citrus waste products (leaves and peels) as natural antioxidants with antiinflammatory properties for medicinal value and volatile oils compounds for aroma in beverages and cosmetic industries.
- Full Text:
- Date Issued: 2019
- Authors: Matuka, Thandiswa
- Date: 2019
- Subjects: Phytochemical -- Analysis Biology -- Leaves and peels Citrus species -- Eastern Cape -- South Africa
- Language: English
- Type: Thesis , Masters , M Sc
- Identifier: http://hdl.handle.net/11260/1362 , vital:32263
- Description: Citrus sinensis, Citrus reticulata and Citrus clementine are citrus species that belong to a large family Rutaceae. Citrus leaves and peels are often regarded as waste products. In view of the environment pollution impact, the search for end use of citrus waste products for economic value and medicinal purposes was sort after. The aim of this study was to investigate chemical composition of citrus waste products (leaves and peels) essential oils and determine biological active volatile compounds from Citrus sinensis, Citrus reticulata and Citrus clementine Leaves and peels of Citrus sinensis, Citrus reticulata and Citrus clementine collected twice from citrus farm were extracted for essential oils using hydrodistillation method and analysed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) for plant samples collected in 2016 and, gas chromatography (GC) and gas chromatography-triple quadrupole-mass spectrometry (GC-MSMS) for plant samples collected in 2017. The oil yield for Citrus sinensis was 0.19 - 0.85 %v/w for fresh and dried leaf and peels for samples collected in 2016 and 0.26 – 0.87% v/w for those collected in 2017. Analysis of the essential oils analyses identified sabinene (20.4% GCMS, 22.6% GCMSMS), terpinen-4-ol (13.2% GCMS, 6.8% GCMSMS) and linalool (7.6% GCMS, 19.3% GCMSMS) as major compounds in fresh leaves essential oils while β-elemene (16.3% GCMS, 21.4% GCMSMS) and sabinene (10.7% GCMS, 20.8% GCMSMS) were major compounds characterized in dried leaves essential oils. Fresh and dried peels essential oils had limonene as the major constituent (49.6% GCMS, 87.1% GCMSMS) and (73.6% GCMS, 88.3% GCMSMS) respectively. Citrus reticulata essential oils percentage yield range between 0.44-0.80% v/w for fresh and dried leaf and peel oils. Analyses of the GCMS and GCMSMS chromatogram of the volatile oils analyses revealed linalool (33.4% GCMS, 19.6% GCMSMS), (+)-spathulenol (15.2% GCMS, 10.1% GCMSMS) and terpinene-4-ol (4.5% GCMS, 11.9% GCMSMS) as major compounds in fresh leaf essential oils while, sabinene (20.8% GCMS, 32.5% GCMSMS) and linalool (17.2% GCMS, 20.4% GCMSMS) were the major compounds found in dried leaf essential oils. Limonene (55.2%, 75.4% GCMS; 39.5%, 80.8% GCMSMS) was the major prominent compound identified in the C. reticulata peel essential oils in fresh and dried peels respectively, followed by linalool (12.8%, 2.9% GCMS; 48.4%, 7.4% GCMSMS). Percentage yields for the essential oils from C. clementine fresh and dried leaves and peels ranges from 0.40-0.62% v/w for. Fresh and dried leaves volatile oils had sabinene (22.7-27.1%) for GCMS and GCMSMS respectively), linalool (18.5- 20.9% GCMS and GCMSMS) and terpinene-4-ol (6.5- 11.8% GCMS and GCMSMS) as main compounds while fresh and dried peels oils were dominated by limonene (38.9- 86.5% GCMS and GCMSMS). Phytochemical screening experiment of the three citrus species leaves and peels aqueous extract reveal the prominent presence of flavonoids and phenolic compounds in high concentrations. C. reticulata and C. clementine peels and leaves volatile oils exhibited higher antioxidant activity as DPPH free radical scavengers and Ferric reducing power (FRAP) ability, when compared to C. sinensis essential oils which exhibited minimum antioxidant activity. Antioxidant activity of leaves and peels essential oils maybe due to the presence of oxygenated monoterpenes and high limonene content. C. sinensis peels oils showed non-toxic to extremely very low toxicity effect at highest dose 2000 mg/kg. C. reticulata and C. clementine peels oils showed toxicity effect in mice at high dose levels. It is suggested that citrus volatile oils could be safe to use at lower dose levels. Citrus species peels and leaves volatile compounds limonene linalool and sabinene exhibited antiinflammatory activity by significantly reducing rats paw oedema size induced by fresh egg albumin. The study suggests the use of Citrus waste products (leaves and peels) as natural antioxidants with antiinflammatory properties for medicinal value and volatile oils compounds for aroma in beverages and cosmetic industries.
- Full Text:
- Date Issued: 2019
Seasonal variations of essential oil composition and some biological evaluation of Pelargonium inquinans (L.) Ait. South Africa
- Authors: Tembeni, Babalwa
- Date: 2016
- Subjects: Pelargoniums Essences and essential oils Medicinal plants
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/6281 , vital:29537
- Description: Pelargonium inquinans which belongs to the family Geraniaceae, is an essential oil yielding plant. P. inquinans was collected from two different sites in Grahamstown (wild) and Alice, at the University of Fort Hare botanical garden. Authentication of the plant was done by Dr. T. Dold at Schonland herbarium, and the voucher specimen being T01. This study is focused at determining the chemical constituents and biological properties of the essential oils of wild and cultivated P. inquinans across different seasons, as the plant grows throughout the year. Seasonal collection of wild and cultivated P. inquinans was achieved in a duration of 12 months. 32 essential oil samples of P. inquinans were obtained by extraction using hydro-distillation technique for 3-4 hours. The chemical composition of the essential oils was determined using GC/MS and GC/FID. Amongst the 32 essential oils only 4 samples wild ( fresh stem and leaf) and cultivated ( fresh stem and leaf) from summer season were evaluated for analgesic activity using acetic acid induced writhings and hot plate nociception models in mice, anti-inflammatory activity was determined on the egg albumin- induced rat paw oedema in rats. The results obtained from GC-MS revealed a total of 169 components obtained from the leafstem, fresh/dry wild and cultivated P. inquinans. These essential oils showed a great deal of chemotaxonomic variation and similarity in the major and minor components along the season. In spring season the essential oils of wild and cultivated P. inquinans had abundance of hydrogenated sesquiterpenes (20.6percent-66.7percent). The major components were found to be α-caryophyllene (9.1percent-26.8percent), p-xylene (23.3percent-27.5percent), β-caryophyllene (11.4percent-30.9percent), o-xylene (6.3percent-39.4percent), β-thujene (8.7percent), isocaryophyllene (13.9percent), isoborneol (14.2percent), β-myrcene (5.7percent), geranyl acetate (13.8percent), toluene (7.9percent), β-gurjunene (18.5percent), α-cadinene (15.8percent), β-farnesene (14.2percent), 3-carene (12.1percent) and camphene (9.0percent). In summer season the essential oils of wild and cultivated P. inquinans were found to have abundance of hydrogenated sesquiterpenes (50.3percent- 63.0percent), oxygenated monoterpenes (30.4percent) and hydrogenated monoterpenes (20.8percent- 61.0percent). The major components were found to be α-caryophyllene (12.3percent-25.8percent), β-caryophyllene (15.1percent- 31.7percent), trans-caryophyllene (10.3percent- 17.8percent), phytol (14.2percent- 20.2percent), camphor (46.5percent), sabinene (27.8percent), elemol (18.1percent), z3-hexenyl isobutyrate (16.3percent), limonene (12.1percent), menthone (12.1percent)< E.E-β-farnesene (14.7percent), palmitic acid (9.6percent), eugenol (9.4percent), cis- β-ocimene (8.7percent), α-terpineol (8.7percent), geranyl acetone (7.8percent), β- humulene (7.5percent). linoleic acid (7.4percent), trans-linalool oxide (7.4percent), β-bisabolene (7.1percent), cis- linalool oxide (7.1percent), ionone (6.9percent), caryophyllene oxide (6.9percent) and germacrene d (6.3percent). In autumn season the essential oils of wild and cultivated P. inquinans were found to be rich in hydrogenated sesquiterpenes (29.3percent- 65.2percent) and oxygenated sesquiterpenes (22.8percent- 31.4percent). The major components were found to be α-caryophyllene (15.5percent- 23.4percent), β-caryophyllene (15.2percent- 17.2percent), β-myrcene (7.7percent-13.8percent), β-humulene (8.7percent- 15.2percent), caryophyllene oxide (9.8- 16.2percent), trans- caryophyllene (16.7percent- 23.3percent), α-humulene (11.8percent- 18.6percent), linoleic acid (11.2percent), palmitic acid (10.4percent), phytol acetate (8.5percent), -longipinene (8.3percent) and citronellol (7.8percent). In winter season the essential oils of wild and cultivated P. inquinans were found to have abundance of hydrogenated sesquiterpenes (25.1percent- 48.6percent), oxygenated monoterpenes (47.6percent), oxygenated sesquiterpenes (22.2percent- 28.0percent). The major components were found to be β-caryophyllene (14.6percent- 23.0percent), α-caryophyllene (9.4percent- 18.0percent), trans- caryophyllene (12.2percent- 14.6percent), α-cedrene (26.2percent), germacrene –d-4-ol (16.8percent), 2,6-dihydroxyacetophenone (15.6percent), (+) epibicyclosesquiphellandrene (15.3percent), E-β- farnesene (13.0percent), β-phellandrene (11.7percent), 2-nitrophenol (9.5percent), palatinol (8.4percent), geranyl acetate (7.7percent) and linoleic acid (7.4percent). The oils from the wild and cultivated sources showed significant (p<0.05-0.001) decrease in number of writhes induced by the acetic acid compared to vehicle; caused significant (p<0.05-0.001) delay in reaction time on the hot plate at 60 and 90 min post-treatment and significantly (p<0.05-0.001) reduced oedema size caused by the egg albumin injection compared to the vehicle. The oils from the wild plant showed more potency compared to the cultivated. The essential oils of wild and cultivated P. inquinans showed qualitative, quantitative and chemotaxonomic variation with analgesic and anti-inflammatory activity. These essential oils need to be explored for further biological analysis because of the major components they contain.
- Full Text:
- Date Issued: 2016
- Authors: Tembeni, Babalwa
- Date: 2016
- Subjects: Pelargoniums Essences and essential oils Medicinal plants
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/6281 , vital:29537
- Description: Pelargonium inquinans which belongs to the family Geraniaceae, is an essential oil yielding plant. P. inquinans was collected from two different sites in Grahamstown (wild) and Alice, at the University of Fort Hare botanical garden. Authentication of the plant was done by Dr. T. Dold at Schonland herbarium, and the voucher specimen being T01. This study is focused at determining the chemical constituents and biological properties of the essential oils of wild and cultivated P. inquinans across different seasons, as the plant grows throughout the year. Seasonal collection of wild and cultivated P. inquinans was achieved in a duration of 12 months. 32 essential oil samples of P. inquinans were obtained by extraction using hydro-distillation technique for 3-4 hours. The chemical composition of the essential oils was determined using GC/MS and GC/FID. Amongst the 32 essential oils only 4 samples wild ( fresh stem and leaf) and cultivated ( fresh stem and leaf) from summer season were evaluated for analgesic activity using acetic acid induced writhings and hot plate nociception models in mice, anti-inflammatory activity was determined on the egg albumin- induced rat paw oedema in rats. The results obtained from GC-MS revealed a total of 169 components obtained from the leafstem, fresh/dry wild and cultivated P. inquinans. These essential oils showed a great deal of chemotaxonomic variation and similarity in the major and minor components along the season. In spring season the essential oils of wild and cultivated P. inquinans had abundance of hydrogenated sesquiterpenes (20.6percent-66.7percent). The major components were found to be α-caryophyllene (9.1percent-26.8percent), p-xylene (23.3percent-27.5percent), β-caryophyllene (11.4percent-30.9percent), o-xylene (6.3percent-39.4percent), β-thujene (8.7percent), isocaryophyllene (13.9percent), isoborneol (14.2percent), β-myrcene (5.7percent), geranyl acetate (13.8percent), toluene (7.9percent), β-gurjunene (18.5percent), α-cadinene (15.8percent), β-farnesene (14.2percent), 3-carene (12.1percent) and camphene (9.0percent). In summer season the essential oils of wild and cultivated P. inquinans were found to have abundance of hydrogenated sesquiterpenes (50.3percent- 63.0percent), oxygenated monoterpenes (30.4percent) and hydrogenated monoterpenes (20.8percent- 61.0percent). The major components were found to be α-caryophyllene (12.3percent-25.8percent), β-caryophyllene (15.1percent- 31.7percent), trans-caryophyllene (10.3percent- 17.8percent), phytol (14.2percent- 20.2percent), camphor (46.5percent), sabinene (27.8percent), elemol (18.1percent), z3-hexenyl isobutyrate (16.3percent), limonene (12.1percent), menthone (12.1percent)< E.E-β-farnesene (14.7percent), palmitic acid (9.6percent), eugenol (9.4percent), cis- β-ocimene (8.7percent), α-terpineol (8.7percent), geranyl acetone (7.8percent), β- humulene (7.5percent). linoleic acid (7.4percent), trans-linalool oxide (7.4percent), β-bisabolene (7.1percent), cis- linalool oxide (7.1percent), ionone (6.9percent), caryophyllene oxide (6.9percent) and germacrene d (6.3percent). In autumn season the essential oils of wild and cultivated P. inquinans were found to be rich in hydrogenated sesquiterpenes (29.3percent- 65.2percent) and oxygenated sesquiterpenes (22.8percent- 31.4percent). The major components were found to be α-caryophyllene (15.5percent- 23.4percent), β-caryophyllene (15.2percent- 17.2percent), β-myrcene (7.7percent-13.8percent), β-humulene (8.7percent- 15.2percent), caryophyllene oxide (9.8- 16.2percent), trans- caryophyllene (16.7percent- 23.3percent), α-humulene (11.8percent- 18.6percent), linoleic acid (11.2percent), palmitic acid (10.4percent), phytol acetate (8.5percent), -longipinene (8.3percent) and citronellol (7.8percent). In winter season the essential oils of wild and cultivated P. inquinans were found to have abundance of hydrogenated sesquiterpenes (25.1percent- 48.6percent), oxygenated monoterpenes (47.6percent), oxygenated sesquiterpenes (22.2percent- 28.0percent). The major components were found to be β-caryophyllene (14.6percent- 23.0percent), α-caryophyllene (9.4percent- 18.0percent), trans- caryophyllene (12.2percent- 14.6percent), α-cedrene (26.2percent), germacrene –d-4-ol (16.8percent), 2,6-dihydroxyacetophenone (15.6percent), (+) epibicyclosesquiphellandrene (15.3percent), E-β- farnesene (13.0percent), β-phellandrene (11.7percent), 2-nitrophenol (9.5percent), palatinol (8.4percent), geranyl acetate (7.7percent) and linoleic acid (7.4percent). The oils from the wild and cultivated sources showed significant (p<0.05-0.001) decrease in number of writhes induced by the acetic acid compared to vehicle; caused significant (p<0.05-0.001) delay in reaction time on the hot plate at 60 and 90 min post-treatment and significantly (p<0.05-0.001) reduced oedema size caused by the egg albumin injection compared to the vehicle. The oils from the wild plant showed more potency compared to the cultivated. The essential oils of wild and cultivated P. inquinans showed qualitative, quantitative and chemotaxonomic variation with analgesic and anti-inflammatory activity. These essential oils need to be explored for further biological analysis because of the major components they contain.
- Full Text:
- Date Issued: 2016
Isolation, Characterisation, and biological activity evaluation of essential Oils of Cymbopogon validus (Stapf) Stapf ex Burtt Davy and Hyparrhenia hirta (L.) Stapf
- Authors: Rungqu, Pamela
- Date: 2014
- Language: English
- Type: Thesis , Masters , MSc (Chemistry)
- Identifier: vital:11354 , http://hdl.handle.net/10353/d1021283
- Description: Cymbopogon validus and Hyparrhenia hirta belong to the Poaceae botanical family. Both plants are used as thatching material; H. hirta is also used for weaving mats and baskets. In this study, we investigated the anti-inflammatory effects of C. validus and H. hirta essential oils on fresh egg-albumin induced edema on Wistar rats. To fully understand the chemically induced anti-inflammatory properties of these plants, we first analyzed the chemical composition of the essential oils. The essential oils were analyzed using gas chromatography-mass spectrometry (GC-MS). In C. validus, 13 compounds accounted for 74.3% of fresh leaves oil, 14 compounds 71.8% of dried leaves oil and 12 compounds 73.3% of flower oil were identified from the GC-MS Chromatogram. The percentage yields were as follows fresh leaves oil 2.2%, dried leaves oil 2.0% and flower oil 2.4% v/w respectively. Linalool (3.2-29.6%) and northujane (4.4-16.9%) were the dominant compounds found in the 3 oils analyzed. While, α-terpineol 37.5% and verbenone 13.5% was only found in the fresh leaf oil, this was absent in the dried leaves oil and the flowers oil of C. validus. In H. hirta, 25 compounds accounted for 68.1% of fresh leaves oil, 40 compounds 71.9% of dried leaves oil, 23 compounds 77.6% of fresh flowers oil and 18 compounds 80.1% of dried flowers oil were identified from the GC-MS Chromatogram. The percentage yields obtained from the different parts were 3.4% for fresh leaves oil, 2.8% for dried leaves oil, 2.8% for fresh flowers oil and 0.7% for dried flowers oil v/w respectively. Northujane (8.5-30.0%), diisooctyl phthalate (4.4-26.5%), phytone (1.1-10.4%) were the dominant compounds found in the 4 oils analyzed. While, dibutyl phthalate 26.9% was only found in the fresh flowers and was absent in the dried flowers oil. Moreover, caryophyllene oxide (1.7-9.6%) was found in fresh leaves and dried flowers oil of H. hirta. In vivo analysis revealed that the two essential oils displayed significant edema inhibition effect overtime. They displayed strong anti-inflammatory properties when compared to control group. However, the H. hirta essential oil was more effective than that of C. validus. Linalool, α-terpineol, and northujane extracted from C. validus and H. hirta essential oils might have contributed to the anti-inflammatory effects observed in Wistar rats. This study, confirms the anti-inflammatory properties of C. validus and H. hirta suggesting that they may be used in diseases related to anti-inflammation.
- Full Text:
- Date Issued: 2014
- Authors: Rungqu, Pamela
- Date: 2014
- Language: English
- Type: Thesis , Masters , MSc (Chemistry)
- Identifier: vital:11354 , http://hdl.handle.net/10353/d1021283
- Description: Cymbopogon validus and Hyparrhenia hirta belong to the Poaceae botanical family. Both plants are used as thatching material; H. hirta is also used for weaving mats and baskets. In this study, we investigated the anti-inflammatory effects of C. validus and H. hirta essential oils on fresh egg-albumin induced edema on Wistar rats. To fully understand the chemically induced anti-inflammatory properties of these plants, we first analyzed the chemical composition of the essential oils. The essential oils were analyzed using gas chromatography-mass spectrometry (GC-MS). In C. validus, 13 compounds accounted for 74.3% of fresh leaves oil, 14 compounds 71.8% of dried leaves oil and 12 compounds 73.3% of flower oil were identified from the GC-MS Chromatogram. The percentage yields were as follows fresh leaves oil 2.2%, dried leaves oil 2.0% and flower oil 2.4% v/w respectively. Linalool (3.2-29.6%) and northujane (4.4-16.9%) were the dominant compounds found in the 3 oils analyzed. While, α-terpineol 37.5% and verbenone 13.5% was only found in the fresh leaf oil, this was absent in the dried leaves oil and the flowers oil of C. validus. In H. hirta, 25 compounds accounted for 68.1% of fresh leaves oil, 40 compounds 71.9% of dried leaves oil, 23 compounds 77.6% of fresh flowers oil and 18 compounds 80.1% of dried flowers oil were identified from the GC-MS Chromatogram. The percentage yields obtained from the different parts were 3.4% for fresh leaves oil, 2.8% for dried leaves oil, 2.8% for fresh flowers oil and 0.7% for dried flowers oil v/w respectively. Northujane (8.5-30.0%), diisooctyl phthalate (4.4-26.5%), phytone (1.1-10.4%) were the dominant compounds found in the 4 oils analyzed. While, dibutyl phthalate 26.9% was only found in the fresh flowers and was absent in the dried flowers oil. Moreover, caryophyllene oxide (1.7-9.6%) was found in fresh leaves and dried flowers oil of H. hirta. In vivo analysis revealed that the two essential oils displayed significant edema inhibition effect overtime. They displayed strong anti-inflammatory properties when compared to control group. However, the H. hirta essential oil was more effective than that of C. validus. Linalool, α-terpineol, and northujane extracted from C. validus and H. hirta essential oils might have contributed to the anti-inflammatory effects observed in Wistar rats. This study, confirms the anti-inflammatory properties of C. validus and H. hirta suggesting that they may be used in diseases related to anti-inflammation.
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- Date Issued: 2014
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